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Cloning of a dsp gene from Xanthomonas oryzae pv.oryzicola(PDF)


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Cloning of a dsp gene from Xanthomonas oryzae pv.oryzicola
LAI Zhi bing SHAO Min SONG Cong feng CHEN Gong you WANG Jin sheng
Key Laboratory of Monitoring, Management of Plant Diseases, Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
Xanthomonas oryzae pv oryzicola mutagenesis dsp genes function complementation
A dsp (disease specific pathogenicity) mutant, AM11, of Xanthomonas oryzae pv oryzicola (Xooc)was obtained following mutagenesis of the wild type Xooc strain RS105 with diethyl sulfate. The mutant was similar to the wild type in colonial morphology, color, the production of extracellular polysaccharides, and activities of several extracellular enzymes(amylases, pectate lyases, proteases, and cellulases). Based on complementation by biparental conjugation of AM11 with each of 1 200 cosmid clones from RS105 genomic library, the cosmid pC101 was found to restore the mutant pathogenicity on rice to the wild type strain RS105.Digestion analysis of pC101 indicated that pC101 contain a 44 92 kb dsp gene fragment. Following subcloning and function complementation, two subclones( pE46 and pE47)restored the mutant pathogenicity as the RS105.Therefore, it is suggested that Xooc contains at least two dsp genes.


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Last Update: 2004-02-28