[1]林茂松.室内人工接种测定甘薯品种对马铃薯腐烂线虫的抗性[J].南京农业大学学报,1989,12(03):44-47.[doi:10.7685/j.issn.1000-2030.1989.03.009]
 Lin Maosong.TESTING THE RESISTANCE OF SWEET-POTATO VARIETIES TO POTATO-ROT NEMATODE BY ARTIFICIAL INOCULATION[J].Journal of Nanjing Agricultural University,1989,12(03):44-47.[doi:10.7685/j.issn.1000-2030.1989.03.009]
点击复制

室内人工接种测定甘薯品种对马铃薯腐烂线虫的抗性()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
12卷
期数:
1989年03期
页码:
44-47
栏目:
OA栏目
出版日期:
1989-08-30

文章信息/Info

Title:
TESTING THE RESISTANCE OF SWEET-POTATO VARIETIES TO POTATO-ROT NEMATODE BY ARTIFICIAL INOCULATION
作者:
林茂松
南京农业大学植物病理教研组
Author(s):
Lin Maosong
Section of Plant Pathology, Nanjing Agricultural University
关键词:
甘薯 马玲薯腐烂线虫 抗性
Keywords:
Sweet-potato Potato rot nematode Resistance
DOI:
10.7685/j.issn.1000-2030.1989.03.009
文献标志码:
A
摘要:
将毛壳菌(Chaetomium sp.)上培养繁殖的马铃薯腐烂线虫(Ditylenchus destructor Thorne,1945),经表面消毒处理后配成一定浓度的悬浮液,用管法人工接种甘薯薯块。薯块的最适接种虫量为500条,温度25℃。接种后30天观察。以薯块横切面发病程度和线虫繁殖量作为分级标准。试验证明这种方法可以简便、快速、准确地测定甘薯品种对该线虫的抗性。
Abstract:
Potato-rot nematode(Ditylenchus destructor Thorne, 1945)was propagated by culturing on the fungus (Chaetomium sp.) and definite number of the nematode was inoculated into the center of the tuber of the tested sweetpotato varieties. It was found that the optimum inoculated quantity is 500 nematodes for each tuber and the suitable observation time is 30 days (25℃) after inoculation. The degree of resistance is judged by percentage of the spongy area around the inoculated point. The degree of resistance of sweet-potato varieties judged by the artificial inoculation in the laboratory agree fairly well with the ordinary field testing method, but this method is more accurate and fast and large number of sweet-potato varieties can be tested in short time.

相似文献/References:

[1]王钰,王荣富,何国浩.甘薯抗病基因同源序列的克隆与分析[J].南京农业大学学报,2008,31(3):81.[doi:10.7685/j.issn.1000-2030.2008.03.016]
 WANG Yu,WANG Rong-fu,HE Guo-hao.Cloning of resistance gene analog(RGA)from sweetpotato(Ipomoea batatas(L.) Lam)[J].Journal of Nanjing Agricultural University,2008,31(03):81.[doi:10.7685/j.issn.1000-2030.2008.03.016]
[2]张明生,杜建厂,谢波,等.水分胁迫下甘薯叶片渗透调节物质含量与品种抗旱性的关系[J].南京农业大学学报,2004,27(4):123.[doi:10.7685/j.issn.1000-2030.2004.04.028]
 ZHANG Ming-sheng,DU Jian-chang,XIE Bo,et al.Relationship between osmoregulation substance in sweet potato under water stress and variety drought resistance[J].Journal of Nanjing Agricultural University,2004,27(03):123.[doi:10.7685/j.issn.1000-2030.2004.04.028]
[3]闫明明,徐碧莲,吴琼,等.高效低耗的甘薯脱毒苗快繁技术研究[J].南京农业大学学报,2015,38(4):689.[doi:10.7685/j.issn.1000-2030.2015.04.025]
 YAN Mingming,XU Bilian,WU Qiong,et al.Study on the high efficiency and low-expensive rapid propagation technology in virus-free sweet potato seedling[J].Journal of Nanjing Agricultural University,2015,38(03):689.[doi:10.7685/j.issn.1000-2030.2015.04.025]

更新日期/Last Update: 1989-08-30