[1]孙捷,杨寿运,崔春亮,等.基于分子证据探讨松材线虫在中国的传播途径[J].南京农业大学学报,2008,31(2):55-60.[doi:10.7685/j.issn.1000-2030.2008.02.012]
 SUN Jie,YANG Shou-yun,CUI Chun-liang,et al.Possible transmission routes of Bursaphelenchus xylophilus in China based on molecular data[J].Journal of Nanjing Agricultural University,2008,31(2):55-60.[doi:10.7685/j.issn.1000-2030.2008.02.012]
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基于分子证据探讨松材线虫在中国的传播途径()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
31卷
期数:
2008年2期
页码:
55-60
栏目:
出版日期:
2008-05-30

文章信息/Info

Title:
Possible transmission routes of Bursaphelenchus xylophilus in China based on molecular data
作者:
孙捷杨寿运崔春亮张崇星林茂松张克云
南京农业大学生命科学学院,江苏,南京,210095;南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室,江苏,南京,210095
Author(s):
SUN Jie YANG Shou-yun CUI Chun-liang ZHANG Chong-xing LIN Mao-song ZHANG Ke-yun
College of Life Sciences/College of Plant Protection/Key Laboratory of Monitoring and Management of Plant Diseasesand Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
松材线虫病 松材线虫 RAPD 传播途径
Keywords:
pine wood nematode disease Bursaphelenchus xylophilus RAPD transmission route
分类号:
S763.1
DOI:
10.7685/j.issn.1000-2030.2008.02.012
文献标志码:
A
摘要:
采用11个随机引物对来自中国最早发病的5个省和日本疫区的松材线虫共10个居群进行遗传多样性分析和系统发育分析,拟从分子水平探讨松材线虫病在中国蔓延的可能途径。结果表明:中国松材线虫居群间存在明显的遗传差异,遗传距离为0.066~0.262;除了安徽明光的居群自成一支外,其他居群均与南京居群聚成一支,其中安徽皖南和浙江岱山居群与南京中山陵居群遗传距离最近,为0.066;安徽明光居群与南京居群的遗传距离最大,为0.262。表明发生在安徽皖南和浙江岱山等地的松材线虫病可能是由最早发病的南京地区扩散的;而发生在毗邻南京的安徽明光的松材线虫病不是由南京地区扩散的。该结果揭示,松木制品的调运等人为远距离传播是松材线虫在我国蔓延的主要途径之一。
Abstract:
In order to reveal possible transmission routes of Bursaphelenchus xylophilus in China, genetic diversity and phylogenetic analysis of 9 B. xylophilus populations from 5 provinces in China and one population in Japan were conducted based on RAPD of 11 random primers. Genetic diversity analysis showed that there was significant genetic variation among different populations of B. xylophilus in China, genetic distances between these populations were from 0.066 to 0.262;the genetic distance between population of Nanjing and Mingguang, Anhui Province, was the largest(0.262), whereas the genetic distance between population of Nanjing and South Anhui and Daishan, Zhejiang Province was the smallest(0.066). Phylogenetic analysis revealed that populations of B. xylophilus in China were divided into two clades:B. xylophilus populations from Nanjing, Zhejiang, Guangdong, Shandong, and South Anhui formed one clade;another one was made up only by the population of B. xylophilus in Mingguang, which adjacent to Nanjing. It suggested that the origin of pine wood nematode disease in Mingguang was different from that of Nanjing, whereas occurrences of pine wood nematode disease in the other places in China were probably dispersed from Nanjing, where pine wood nematode disease happened firstly in China, which suggested that transmission by imported pine wood packing boards was one of the most important transmission way of B. xylophilus in China.

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备注/Memo

备注/Memo:
国家高技术研究发展计划(2001AA249021);江苏省科技攻关项目(BE99359);中国博士后科学基金(20060390292);江苏省博士后科学基金(0601030B)
更新日期/Last Update: 2008-05-30