[1]陈海旺,侯喜林*,李英,等.冷胁迫下不结球白菜BcSLAC1 基因克隆和表达分析及ABA 和H2O2 在调控气孔运动中的作用[J].南京农业大学学报,2011,34(4):8.[doi:10.7685/j.issn.1000-2030.2011.04.002]
 CHEN Hai-wang,HOU Xi-lin*,LI Ying,et al.Cloning,expression of BcSLAC1 induced by cold stress in Brassica campestris ssp.chinensis and the role in regulating stomatal movements of ABA and H2O2[J].Journal of Nanjing Agricultural University,2011,34(4):8.[doi:10.7685/j.issn.1000-2030.2011.04.002]
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冷胁迫下不结球白菜BcSLAC1 基因克隆和表达分析及ABA 和H2O2 在调控气孔运动中的作用()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年4期
页码:
8
栏目:
OA栏目
出版日期:
2011-08-31

文章信息/Info

Title:
Cloning,expression of BcSLAC1 induced by cold stress in Brassica campestris ssp.chinensis and the role in regulating stomatal movements of ABA and H2O2
文章编号:
1000-2030(2011)04-0008-05
作者:
陈海旺侯喜林*李英王枫杨学东张硕
南京农业大学作物遗传与种质创新国家重点实验室/ 农业部南方蔬菜遗传改良重点开放实验室,江苏南京210095
Author(s):
CHEN Hai-wangHOU Xi-lin*LI YingWANG FengYANG Xue-dongZHANG Shuo
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetics Improvement, Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China
关键词:
不结球白菜BcSLAC1基因气孔运动荧光观察实时定量PCR
Keywords:
Brassica campestris ssp.chinensisBcSLAC1 genestomatal movementfluorescence observationreal-time quantitative PCR
分类号:
S634.3
DOI:
10.7685/j.issn.1000-2030.2011.04.002
文献标志码:
A
摘要:
采用 RT-PCR 方法首次获得不结球白菜 BcSLAC1 基因 , 该基因核甘酸序列全长 1 668 bp, 其开放阅读框编码 555 个氨基酸。与已公布的拟南芥 SLAC1 基因有较高的同源性。系统进化树分析表明 : SLAHs 基因家族其他基因相比 ,BcSLAC1 基因与拟南芥 SLAC1 基因有更近的遗传距离。在冷胁迫条件下利用气孔开张度测量和荧光显微技术分析表明 : 冷胁迫下 H2O2 可能作为下游信号分子参与了保卫细胞中 ABA 信号转导。实时定量 RT-PCR 检测表明 , 冷胁迫下 BcSLAC1 基因表达量升高 , 同时用 ABA 处理后 ,BcSLAC1 基因表达量比抗坏血酸处理后明显上升 , 说明 ABA BcSLAC1 基因的诱导作用和 ABA 诱导产生的 H2O2 有关。
Abstract:

BcSLAC1 gene was firstly amplified from non-heading Chinese cabbage by RT-PCR method.The nucleotide sequence length of its open reading frame(ORF)was 1 668 bp and encoded 555 amino acid residues,and it showed high homology to Arabidopsis thaliana SLAC1 gene.Phylogenetic tree analysis demonstrated that BcSLAC1 gene had a more recent genetic distance with Arabidopsis thaliana SLAC1 gene compared to the others of SLAHs family.In the cold stress condition,stomatcal aperture bioassay and fluorescence microscopy technology revealed that H2O2 involved as a downstream sigaling molecule of ABA signal transduction to induce stomatal closure in guard cells.Real-time quantitative PCR analysis revealed that the expression of BcSLAC1 gene significantly increased under cold stress and there were significant differences in gene expressions with ABA treatments compared with ascorbic acid treatment.H2O2 was required for the ABA-induced expression of the BcSLAC1 gene in leaves.


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备注/Memo

备注/Memo:
收稿日期:2010-07-23 基金项目:江苏省自然科学基金项目(BK2009311);江苏省农业科技自主创新资金项目(CX(09)604) 作者简介:陈海旺,硕士研究生。*通讯作者:侯喜林,教授,博导,研究方向为蔬菜遗传育种与分子生物学,E-mail:hxl@njau.edu.cn。
更新日期/Last Update: 2011-09-12