[1]刘轶儒,徐菲菲,钱国良,等.产酶溶杆菌rpfG基因的克隆与功能分析[J].南京农业大学学报,2013,36(2):45-50.[doi:10.7685/j.issn.1000-2030.2013.02.008]
 LIU Yiru,XU Feifei,QIAN Guoliang,et al.Clone and functional analysis of gene rpfG in Lysobacter enzymogenes strain OH11[J].Journal of Nanjing Agricultural University,2013,36(2):45-50.[doi:10.7685/j.issn.1000-2030.2013.02.008]
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产酶溶杆菌rpfG基因的克隆与功能分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
36卷
期数:
2013年2期
页码:
45-50
栏目:
出版日期:
2013-03-22

文章信息/Info

Title:
Clone and functional analysis of gene rpfG in Lysobacter enzymogenes strain OH11
作者:
刘轶儒 徐菲菲 钱国良 范加勤 胡白石 刘凤权
南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095
Author(s):
LIU Yiru XU Feifei QIAN Guoliang FAN Jiaqin HU Baishi LIU Fengquan
College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
关键词:
产酶溶杆菌rpfG基因热稳定抗真菌因子实时定量PCR
Keywords:
Lysobacter enzymogenesrpfG geneheat-stable antifungal factorqRT-PCR
分类号:
S432
DOI:
10.7685/j.issn.1000-2030.2013.02.008
摘要:
为探索基因rpfG在产酶溶杆菌中的功能,以产酶溶杆菌OH11为研究对象,在基因组测序的基础上,通过同源比对,在OH11基因组中鉴定出rpfG的同源基因,并命名为rpfGLys。利用同源重组技术,对rpfG基因进行了定向敲除,获得了rpfG的缺失突变株。与野生型相比,rpfG基因的突变降低了产酶溶杆菌重要抗菌物质——热稳定抗菌因子HSAF的产量和对腐霉的拮抗活性。荧光定量PCR显示,在rpfG突变株中负责生物合成HSAF的关键基因Lysegl002651的表达显著下调,与HSAF活性检测结果相吻合。然而,rpfG的突变不改变产酶溶杆菌4种胞外抗菌水解酶的活性。另外,rpfG突变导致产酶溶杆菌菌落表面干燥,但滑行能力提高。
Abstract:
To analyse the function of rpfG in Lysobacter enzymogenes OH11,a paralogous gene of rpfG was identified through sequence homology analysis and named as rpfGLys.With homologous recombination technology,a deletion mutant of rpfG was generated.Compared with the wild type,the mutant had a low secretion of heat stable antibacterial factor(HSAF)which is an important antibacterial substance of Lysobacter enzymogenes and a lower antipathogenic activity toward Pythium aphanidermatum.Fluorogenic quantitative PCR showed that expression of the key gene(Lysegl002651) which is in charge of biosynthesis of HSAF was significantly lower,which coincided with activity detection of HSAF.Further researches found that the mutant did not change the activities of four-type lytic enzymenes of Lysobacter enzymogenes.Moreover,it was of interest that although mutation of rpfG led to acrid surface of colonies,its sliding ability was strengthened.

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备注/Memo

备注/Memo:
收稿日期:2012-5-16;改回日期:。
基金项目:国家公益性行业(农业)科研专项(200903052,201003004,201203034);国家863计划项目(2011AA10A205);国家自然科学基金项目(31101478);教育部高校博士点基金(20100097120013);现代农业产业技术体系(nycytx-29-09);农业部948项目(2010-C18)
作者简介:刘轶儒,硕士研究生。刘凤权,教授,主要从事植物病害生物防治与植物细菌病害,E-mail:fqliu20011@sina.com。
通讯作者:刘凤权,教授,主要从事植物病害生物防治与植物细菌病害,E-mail:fqliu20011@sina.com。
通讯作者:刘凤权,教授,主要从事植物病害生物防治与植物细菌病害,E-mail:fqliu20011@sina.com。
更新日期/Last Update: 1900-01-01