[1]普少瑕,崔宇,李春梅.竹醋液对肉鸡生产性能、生理指标及粪便中氨气的影响[J].南京农业大学学报,2015,38(2):330-338.[doi:10.7685/j.issn.1000-2030.2015.02.023]
 PU Shaoxia,CUI Yu,LI Chunmei.Effects of bamboo vinegar on growth performance,physiological indicators and fecal ammonia in broiler chickens[J].Journal of Nanjing Agricultural University,2015,38(2):330-338.[doi:10.7685/j.issn.1000-2030.2015.02.023]
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竹醋液对肉鸡生产性能、生理指标及粪便中氨气的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
38卷
期数:
2015年2期
页码:
330-338
栏目:
OA栏目
出版日期:
2015-03-23

文章信息/Info

Title:
Effects of bamboo vinegar on growth performance,physiological indicators and fecal ammonia in broiler chickens
作者:
普少瑕1 崔宇2 李春梅1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 江阴中炬生物技术有限公司, 江苏 江阴 214400
Author(s):
PU Shaoxia1 CUI Yu2 LI Chunmei1
1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Jiangyin China Grand Biotechnology Co.Ltd., Jiangyin 214400, China
关键词:
竹醋液肉鸡生产性能血清指标消化酶氨气
Keywords:
bamboo vinegarbroilergrowth performanceserum biochemical indicatorsdigestive enzymesammonia
分类号:
F831
DOI:
10.7685/j.issn.1000-2030.2015.02.023
摘要:
[目的]研究日粮中添加不同水平竹醋液(BV)对肉鸡生产性能、生理变化和粪便中氨气的影响。[方法]选用1日龄AA肉仔鸡192只(公母各半),随机分为4个处理组:对照、BV5、BV10和BV20组,分别在4组的基础日粮中添加0、5、10和20 mL·kg-1的竹醋液。每组6个重复,每个重复8只鸡,试验期为42 d。[结果]各竹醋液组与对照组间肉鸡0~3周体增质量(BWG)和料质量比(F/G)以及0~6周体增质量(BWG)有显著升高(P<0.05);各组间免疫器官指数无显著差异(P>0.05);添加竹醋液可以增加血清总蛋白(TP)和球蛋白(GLB)含量,降低血清白蛋白(ALB)含量和淀粉酶活性(P<0.05或P<0.01);肌胃、腺胃和前肠食糜pH值随着竹醋液添加量的增加而减小(P<0.05或P<0.01);日粮中添加竹醋液引起肉鸡小肠食糜中淀粉酶、胰蛋白酶和糜蛋白酶活性升高,脂肪酶活性随着竹醋液添加量的增加而降低,其中BV5和BV10组糜蛋白酶活性极显著高于对照组(P<0.001),BV10组肉鸡小肠食糜中淀粉酶活性显著高于对照组(P<0.05);添加竹醋液后,十二指肠和空肠的形态发生变化,其中BV10组肉鸡十二指肠绒毛长度、隐窝深度以及空肠绒毛长度均高于其他组,而空肠隐窝深度低于其他组。BV5组肉鸡胸肌pH45 min显著的高于其他3个组(P<0.05);添加竹醋液后肉鸡胸肌亮度(L*)值有所降低,红色度(a*)值有所升高;另外,肉鸡日粮中添加竹醋液后,发酵鸡粪中氨气产生量均有所降低,其中添加BV5组氨气产生量显著低于对照组(P<0.05)。[结论]日粮中添加竹醋液对肉鸡生产性能、血清生化指标、消化道pH值、小肠食糜中消化酶的活性以及肉色均有一定影响;另外竹醋液可减少畜舍中由于鸡粪发酵而产生的氨气;在所有试验组中10 mL·kg-1的竹醋液添加量效果最好。
Abstract:
[Objectives]This study aimed to investigate the effects of basal diets supplemented with different levels of bamboo vinegar(BV)on the growth performance, physiological changes and ammonia contents in the feces of broiler chickens. [Methods]A total of one hundred and ninety-two 1-day-old AA broiler chickens(half male and half female)were randomly divided into 4 treatment groups:control group, BV5, BV10 and BV20 group. Each treatment had 6 replicates with 8 chicks each. Birds were fed with the basal diet supplemented with 0(control group), 5, 10 and 20 mL·kg-1 bamboo vinegar for 42 days. [Results]The results showed a significant difference(P<0.05)between the control group and bamboo vinegar groups in body weight gain(BWG)and feed to gain ratio. No significant differences were observed in immune organ index(P>0.05). Bamboo vinegar increased serum total protein(TP)and globulin(GLB), decreased serum albumin(ALB)and amylase(AMS)activity(P<0.05 or P<0.01). Digestive tracts and chyme pH values were decreased accompanied with the supplementation of bamboo vinegar. Dietary bamboo vinegar increased intestinal chyme amylase(AMS), trypsin and chymotrypsin activity, but the lipase activity was decreased, and the chymotrypsin activity in BV5 and BV10 groups was significantly higher than that in control group(P<0.001), as well as AMS activity in BV10 group(P<0.05). Dietary bamboo vinegar had an effect on villi structure of duodenum and jejunum in broiler chickens(P<0.05). Pectoral pH45min in BV5 group was significantly higher than that in the other 3 groups(P<0.05). After adding bamboo vinegar, pectoral brightness(L*)was decreased, and redness(a*)was increased. Dietary bamboo vinegar significantly decreased fecal ammonia generation. [Conclusions]These results suggested that dietary bamboo vinegar can improve broiler performance, serum biochemical parameters, gastrointestinal pH, intestinal chyme digestive enzyme activity and flesh color. Furthermore, dietary bamboo vinegar can decrease fecal ammonia generation, and improve the farming environment. 10 mL·kg-1 dietary bamboo vinegar has the best effect among 3 experimental groups.

参考文献/References:

[1] Mu J, Uehara T, Furuno T. Effect of bamboo vinegar on regulation of germination and radicle growth of seed plants Ⅱ:composition of moso bamboo vinegar at different collection temperature and its effects[J]. Journal of Wood Science, 2004, 50(5):470-476
[2] 柏明娥, 陈顺伟, 庄晓伟. 不同精制工艺对竹醋液理化性质的影响[J]. 林产化工通讯, 2005, 39(2):25-27 [Bai M E, Chen S W, Zhuang X W. Different refining processes on the physical and chemical properties of the bamboo vinegar[J]. Journal of Chemical Industry of Forest Products, 2005, 39(2):25-27(in Chinese)]
[3] Zahn J A, Hatfield J L, Do Y S, et al. Characterization of volatile organic emissions and wastes from a swine production facility[J]. Environment Quality, 1997, 26, 1687-1696
[4] 吴信, 孔祥峰, 印遇龙, 等. 竹醋液研究进展[J]. 天然产物研究与开发, 2008, 20(5):170-173 [Wu X, Kong X F, Yin Y L, et al. Research progress on bamboo vinegar[J]. Natural Product Research and Development, 2008, 20(5):170-173(in Chinese with English abstract)]
[5] Akakabe Y, Tamura Y, Iwamoto S, et al. Volatile organic compounds with characteristic odor in bamboo vinegar[J]. Bioscience, Biotechnology and Biochemistry, 2006, 70(11):2797
[6] Baimark Y, Niamsa N. Study on wood vinegars for use as coagulating and antifungal agents on the production of natural rubber sheets[J]. Biomass and Bioenergy, 2009, 33(6):994-998
[7] 李凯年. 添加竹醋液对肥育猪生长性能、消化率及其他参数的影响[J]. 中国动物保健, 2013, 15(2):81 [Li K N. The influence of bamboo vinegar on growth performance, digestibility and other parameters of finishing pigs[J]. China Animal Health, 2013, 15(2):81(in Chinese)]
[8] Kook K, Kim J, Jung K, et al. Effect of supplementation bamboo vinegar on production and meat quality of meat-type ducks[J]. Kor J Poultry Science, 2002, 29:293-300
[9] Kook K, Jeong J, Kim K. The effects of supplemental levels of bamboo vinegar liquids on growth performance, serum profile, carcass grade, and meat quality characteristics in finishing pigs[J]. Journal of Animal Science and Technology, 2002, 47:721-730
[10] Ruttanavut J, Yamauchi K, Goto H, et al. Effects of dietary bamboo charcoal powder including vinegar liquid on growth performance and histological intestinal change in Aigamo ducks[J]. International Journal of Poultry Science, 2009, 8(3):229-236
[11] Kishi M, Fukaya M, Tsukamoto Y, et al. Enhancing effect of dietary vinegar on the intestinal absorption of calcium in ovariectomized rats[J]. Bioscience, Biotechnology and Biochemistry, 1999, 63(5):905-910
[12] Benkeblia N, Dahmouni S, Onodera S, et al. Antimicrobial activity of phenolic compound extracts of various onions(Allium cepa L.)cultivars and garlic(Allium sativum L.)[J]. Journal of Food Engineering, 2005, 3:35-40
[13] Choi J, Shinde P, Kwon I, et al. Effect of wood vinegar on the performance, nutrient digestibility and intestinal microflora in weanling pigs[J]. Asian-Australasian Journal of Animal Science, 2009, 22:267-274
[14] 蒋竹英, 李丽立, 唐利华, 等. DON污染饲粮添加竹炭和竹醋液对断奶仔猪抗氧化性能及小肠黏膜形态的影响[J]. 西北农林科技大学学报:自然科学版, 2013, 41(12):37-42 [Jiang Z Y, Li L L, Tang L H, et al. Effect of adding bamboo charcoal and bamboo vinegar to DON contaminated diets on oxidation resistance and small intestinal morphology of weaned piglets[J]. Journal of Northwest A&F University of Science and Technology:Natural Science Edition, 2013, 41(12):37-42(in Chinese with English abstract)]
[15] Qureshi M, Edens F, Havenstein G. Immune system dysfunction during exposure to poult enteritis and mortality syndrome agents[J]. Poultry Science, 1997, 76(4):564-569
[16] 王曼, 陈娟, 孔一力, 等. 丙酮酸肌酸对肉鸡脂肪代谢和蛋白质代谢的影响[J]. 南京农业大学学报, 2011, 34(1):89-94. doi:10.7685/j.issn.1000-2030.2011.01.017 [Wang M, Chen J, Kong Y L, et al. Effects of creatine pyruvate on lipid and protein metabolism in broiler chickens[J]. Journal of Nanjing Agricultural University, 2011, 34(1):89-94(in Chinese with English abstract)]
[17] Cole D, Beal R, Luscombe J. The effect on performance and bacterial flora of lactic acid, propionic acid, calcium propionate and calcium acrylate in the drinking water of weaned pigs[J]. Veterinary Record, 1968, 83(18):459-464
[18] Mroz Z, Jongbloed A, Partanen K, et al. The effects of calcium benzoate in diets with or without organic acids on dietary buffering capacity, apparent digestibility, retention of nutrients, and manure characteristics in swine[J]. Journal of Animal Science, 2000, 78(10):2622-2632
[19] Rafacz-Livingston K, Parsons C, Jungk R. The effects of various organic acids on phytate phosphorus utilization in chicks[J]. Poultry Science, 2005, 84(9):1356-1362
[20] Knekt P, Kumpulainen J, Järvinen R, et al. Flavonoid intake and risk of chronic diseases[J]. The American Journal of Clinical Nutrition, 2002, 76(3):560-568
[21] 钱俊青, 吴宏君, 金再宿, 等. 竹醋液的抗炎活性研究[J]. 浙江工业大学学报, 2012, 40(6):623-625 [Qian J Q, Wu H J, Jin Z S, et al. Study on antiinflammatory activities of bamboo vinegar[J]. Journal of Zhejiang University of Technology, 2012, 40(6):623-625(in Chinese with English abstract)].
[22] Dibner J, Buttin P. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism[J]. The Journal of Applied Poultry Research, 2002, 11(4):453-463
[23] Burnell T, Cromwell G, Stahly T. Effects of dried whey and copper sulfate on the growth responses to organic acid in diets for weanling pigs[J]. Journal of Animal Science, 1988, 66(5):1100-1108
[24] Risley C, Kornegay E, Lindemann M, et al. Effect of feeding organic acids on selected intestinal content measurements at varying times postweaning in pigs[J]. Journal of Animal Science, 1992, 70(1):196-206
[25] 侯永清, 梁敦素, 于斌鹰, 等. 早期断奶仔猪日粮中添加不同种类酸化剂的效果[J]. 中国畜牧杂志, 1996, 32(6):8-11 [Hou Y Q, Liang D S, Yu B Y, et al. Effect of adding different kinds of acidifier in early weaning piglets diet[J]. Chinese Journal of Animal Science, 1996, 32(6):8-11(in Chinese)]
[26] 成令忠. 组织学[M]. 2版. 北京:人民卫生出版社, 1994 [Cheng L Z. Histology[M]. 2nd ed. Beijing:People’s Medical Publishing House, 1994(in Chinese)]
[27] 韩正康. 家畜营养生理学[M]. 北京:农业出版社, 1991 [Han Z K. Livestock Nutrition Physiology[M]. Beijing:Agriculture Press, 1991(in Chinese)]
[28] Yan L, Kim I, Huh K. Influence of bamboo vinegar supplementation on growth performance, apparent total tract digestibility, blood characteristics, meat quality, fecal noxious gas content, and fecal microbial concentration in finishing pigs[J]. Livestock Science, 2012, 144(3):240-246
[29] Yanishlieva-Maslarova N V. Inhibiting oxidation[C]//Yanishlieva N, Pokorny J, Coordon M, et al. Antioxidants in Food:Practical Applications, Sofia, Bulgaria, 2001:22-70
[30] Simitzis P, Deligeorgis S, Bizelis J, et al. Effect of ietary oregano oil supplementation on lamb meat characteristics[J]. Meat Science, 2008, 79(2):217-223
[31] Gladine C, Morand C, Rock E, et al. Plant extracts rich in polyphenols(PERP)are efficient antioxidants to prevent lipoperoxidation in plasma lipids from animals fed n-3 PUFA supplemented diets[J]. Animal Feed Science and Technology, 2007, 136(3):281-296
[32] Stivarius M, Pohlman F, McElyea K, et al. Effects of hot water and lactic acid treatment of beef trimmings prior to grinding on microbial, instrumental color and sensory properties of ground beef during display[J]. Meat Science, 2002, 60(4):327-334
[33] Schefferle H E. The decomposition of uric acid in built up poultry litter[J]. Journal of Applied Microbiology, 1965, 28(3):412-420
[34] Ruttanavut J, Takenoyama S, Yamauch K. Effects of dietary bamboo charcoal powder including vinegar liquid on chicken performance and histological alterations of intestine[J]. Journal of Animal and Feed Sciences, 2010, 19(2):257-268

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备注/Memo

备注/Memo:
收稿日期:2014-6-23。
基金项目:江苏省科技支撑计划项目(BE2012412)
作者简介:普少瑕,硕士研究生。
通讯作者:李春梅,教授,主要从事动物环境生理营养研究,E-mail:chunmeili@njau.edu.cn。
更新日期/Last Update: 1900-01-01