JIANG Yue,SUN Juan,HAN Sidi,et al.Genetic analysis and gene mapping of a yellow leaf mutant yl in rice[J].Journal of Nanjing Agricultural University,2016,39(6):889-897.[doi:10.7685/jnau.201604004]





Genetic analysis and gene mapping of a yellow leaf mutant yl in rice
蒋悦 孙娟 韩思迪 范磊 许凯文 江玲 王春明
南京农业大学作物遗传与种质创新国家重点实验室/江苏省现代作物生产协同创新中心, 江苏 南京 210095
JIANG Yue SUN Juan HAN Sidi FAN Lei XU Kaiwen JIANG Ling WANG Chunming
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing 210095, China
rice(Oryza sativa L.)yellow leaf mutantgene mappingcandidate gene analysischloroplast development
[目的]水稻叶片作为重要的光合器官,是作物产量的形成基础。叶色突变体不仅可以作为育种标记,还是研究叶绿体发育和培育高光效水稻品种的先决条件之一。[方法]从籼稻品种‘9311’的60Co-γ射线辐射诱变突变体库中筛选获得了1个水稻黄叶突变体yl。利用yl/‘宁粳4号’杂交的F2群体进行基因定位。[结果]与野生型相比,yl突变体的幼叶表现出明显的黄化,叶绿素a、叶绿素b、类胡萝卜素的含量下降。突变体叶绿体中类囊体片层结构排列松散。与野生型相比,yl突变体的每穗粒数、结实率和千粒质量下降。该性状受1对隐性核基因控制。该基因初步定位于第9染色体长臂上,进一步开发分子标记把定位区间缩小在14.5 kb内,该区域包含3个候选基因。经测序比对分析发现,yl突变体的第3个ORF的第5外显子发生碱基突变(2131CTA→G),从而造成蛋白第233位氨基酸发生改变并导致翻译提前终止,形成了1个包含原有232个氨基酸和16个新形成氨基酸残基的蛋白,该基因与已报道的水稻黄叶基因YLC1是等位基因。[结论]本研究明确了YLC1参与叶绿素的合成,为叶绿体色素合成和叶绿体发育的研究提供了基础。
[Objectives] Leaf,a crucial organ for photosyhthesis,provides the basis of crop yield formation. The leaf color mutants can be used not only as a trait marker in breeding,but also as one of the prerequisites in chloroplast development and cultivation of new germplasms with higher photosynthetic efficiency. [Methods] A yellow leaf mutant,named as yl,was isolated from the progeny of 60Co-γ-rays treated indica cultivar‘9311’.The gene was isolated using an F2 mapping population derived from the cross of the yl mutant and‘Ningjing 4’. [Results] The yl mutant exhibited a stable yellow leaf phenotype during the seedling stage. As compared with the wild-type,the content of chlorophyll a,chlorophyll b and carotenoid reduced in yl leaves.The yl mutant showed less grain number per panicle,less seed setting rate and fewer 1 000-grain weight. The yellow leaf phenotype of the yl mutant was caused by a single recessive gene located in a 14.5 kb region on chromosome 9 containing three ORFs. The yl gene had nucleotide changes(2130CTA→G) in the fifth exon of the 3rd ORF,which led to frame shift and early termination in the process of translating protein. The gene was a novel allele of the YLC1 gene. [Conclusions] We demonstrated that the YLC1 gene is associated with the chlorophyll synthesis,providing a basis for further study on chloroplast pigment synthesis and chloroplast development.


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更新日期/Last Update: 1900-01-01