[1]刘鑫,黄兴如,张晓霞,等.高浓度多环芳烃污染土壤的微生物-植物联合修复技术研究[J].南京农业大学学报,2017,(4):632-640.[doi:10.7685/jnau.201606036]
 LIU Xin,HUANG Xingru,ZHANG Xiaoxia,et al.Experimental study on plant-microbial remediation of high concentration PAHs-contaminated soil[J].Journal of Nanjing Agricultural University,2017,(4):632-640.[doi:10.7685/jnau.201606036]
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高浓度多环芳烃污染土壤的微生物-植物联合修复技术研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
期数:
2017年4期
页码:
632-640
栏目:
出版日期:
2017-07-07

文章信息/Info

Title:
Experimental study on plant-microbial remediation of high concentration PAHs-contaminated soil
作者:
刘鑫1 黄兴如2 张晓霞2 邱吉国1 徐冬青1 何健1
1. 南京农业大学生命科学学院/农业部农业环境微生物重点实验室, 江苏 南京 210095;
2. 中国农业科学院资源环境与区划研究所, 北京 100081
Author(s):
LIU Xin1 HUANG Xingru2 ZHANG Xiaoxia2 QIU Jiguo1 XU Dongqing1 HE Jian1
1. College of Life Sciences/Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agr
关键词:
多环芳烃土壤微生物-植物联合修复紫花苜蓿盆栽试验大田试验
Keywords:
PAHssoilplant-microbial remediationalfalfapot experimentfield experiment
分类号:
Q939.9
DOI:
10.7685/jnau.201606036
摘要:
[目的]本文旨在研究多环芳烃(PAHs)污染场地微生物-植物联合修复PAHs污染土壤的效果。[方法]利用紫花苜蓿与PAHs降解菌株Rhizobium petrolearium SL-1联合修复土壤中PAHs,设置4个处理:不种苜蓿,不接根瘤菌(CK);不种苜蓿,接根瘤菌(菌);种苜蓿,不接根瘤菌(苜蓿);种苜蓿,接根瘤菌(苜蓿+菌),每个处理设3个重复。盆栽试验土壤取自山东新泰某焦化厂PAHs实地污染土壤,分别于处理20和60 d定期取样;大田试验农田位于河北省唐山市某发电厂附近,分别于处理60和90 d定期取样。测定苜蓿的株高和干质量等生理指标,并利用GC/MS分析土样中的16种PAHs组分降解规律。[结果]盆栽试验中,“苜蓿+菌”处理20和60 d时的苜蓿株高和干质量指标均优于仅种植苜蓿处理;“苜蓿+菌”联合降解PAHs效果明显优于只种植苜蓿或只接菌处理;PAHs不同组分间的降解效果从大到小依次为3环、2环、4环、6环、5环。在大田试验中,60和90 d修复效果同样呈现“苜蓿+菌”联合降解PAHs效果大于只种植苜蓿或只接菌的处理。修复60 d后土壤中低环PAHs的降解率明显高于高环PAHs,PAHs降解效果从大到小依次为2环、3环、4环、5环、6环,但修复90 d后土壤中低环PAHs和高环PAHs的降解率无明显差异。[结论]在PAHs污染土壤或大田试验条件下接种菌株SL-1对紫花苜蓿具有明显的促生作用,并且微生物-植物联合修复比单独微生物或植物修复能更有效地降解PAHs。
Abstract:
[Objectives]The main purpose of this research is to study the bioremediation of polycyclic aromatic hydrocarbons(PAHs)-contaminated soil using plant-microbial remediation systerm. [Methods]The alfalfa and the PAHs-degrading bacterium Rhizobium petrolearium SL-1 were used. One control(CK)was set with noninoculation of alfalfa and strain SL-1. Three treatments were set as follows:inoculation with only alfalfa(alfalfa),inoculation with only strain SL-1(strain),and inoculation of both alfalfa and strain SL-1(alfalfa+strain),respectively. The PAHs-contaminated soil was collected in a coking plant in Xintai,Shandong for the pot experiments. The field experiments were performed on a farm near a power plant in Tangshan,Hebei. The samples were observed at the 20 and 60 d for the pot experiments,and at 60 and 90 d for the field experiments. Physiological indexes,the plant height and dry weight were determined. The content of 16 kinds of PAHs component in soil samples was monitored by GC/MS. [Results]The physiological indexes such as plant height and dry weight of alfalfa in the treatment(alfalfa+strain)were better than those of treatment(alfalfa)at the 20 and 60 d in pot experiment. The PAHs degradation efficiency of the treatment(alfalfa+strain)was significantly higher than those of the treatment only planting alfalfa or inoculating strain. The degradation efficiency for different PAHs ranged from high to low were:3 ring,2 ring,4 ring,6 ring,5 ring. In the field experiment,the treatment(alfalfa+strain)also showed a better remediation results than treatment(alfalfa)or(strain)at the 60 and 90 d. The degradation rate of low-molecular-weight PAHs at 60 d was significantly higher than that of high-molecular-weight PAHs in soil. After 90 d the degradation rate of PAHs was no significantly different among the 16 kinds of PAHs. [Conclusions]Inoculation of strain SL-1 promotes the growth of alfalfa in the in-situ conditions. Combined remediation of strain SL-1 and alfalfa has better remediation efficiency towards PAH-contaminated soil than single strain SL-1 or alfalfa bioremediation.

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备注/Memo

备注/Memo:
收稿日期:2016-06-28。
基金项目:国家863计划项目(2012AA101403);引进国际先进农业科学技术重点项目(2011-G25)
作者简介:刘鑫,硕士研究生。
通信作者:何健,教授,博导,主要从事有机污染土壤生物修复技术研究,E-mail:hejian@njau.edu.cn。
更新日期/Last Update: 2017-07-07