[1]朱碧春,顾丽,李正,等.南极土壤芽孢杆菌的分离鉴定及其防治玉米细菌性褐腐病的研究[J].南京农业大学学报,2017,(4):641-648.[doi:10.7685/jnau.201611008]
 ZHU Bichun,GU Li,LI Zheng,et al.Isolation and identification of the Antarctic soil Bacillus and its biocontrol effect against brown stalk rot on maize[J].Journal of Nanjing Agricultural University,2017,(4):641-648.[doi:10.7685/jnau.201611008]
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南极土壤芽孢杆菌的分离鉴定及其防治玉米细菌性褐腐病的研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
期数:
2017年4期
页码:
641-648
栏目:
出版日期:
2017-07-07

文章信息/Info

Title:
Isolation and identification of the Antarctic soil Bacillus and its biocontrol effect against brown stalk rot on maize
作者:
朱碧春 顾丽 李正 伍辉军 顾沁 吴黎明 高学文
南京农业大学植物保护学院/华东作物有害生物综合治理农业部重点实验室, 江苏 南京 210095
Author(s):
ZHU Bichun GU Li LI Zheng WU Huijun GU Qin WU Liming GAO Xuewen
College of Plant Protection/Key Laboratory of Integrated Pest Management on Crops in East China, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
南极芽孢杆菌gyrB基因生防菌筛选脂肽化合物
Keywords:
AntarcticBacillus spp.gyrB genescreening of biocontrol agentslipopeptide compands
分类号:
S476
DOI:
10.7685/jnau.201611008
摘要:
[目的]本研究旨在从南极土壤中筛选防治玉米细菌性褐腐病的芽孢杆菌菌株,为该病害的防治提供菌株资源。[方法]从南极长城站地震台附近的企鹅聚居地土壤中分离芽孢杆菌。结合16S rDNA及gyrB基因序列对分离到的芽孢杆菌进行鉴定。借助平板抑菌试验筛选对玉米细菌性褐腐病病原菌具有拮抗效果的生防芽孢杆菌。通过田间防治试验,筛选具有较好生防效果的芽孢杆菌。利用MALDI-TOF-MS对芽孢杆菌产生的抗菌物质脂肽化合物进行鉴定。[结果]通过16S rDNA及gyrB基因序列对分离自南极的23株芽孢杆菌进行鉴定,其中解淀粉芽孢杆菌(Bacillus amyloliquefaciens)9株,地衣芽孢杆菌(Bacillus licheniformis)14株。借助平板抑菌试验,筛选获得抑菌效果明显的2株B.amyloliquefaciens菌株EZ15-07、EZ15-09和2株B.licheniformis菌株EZ01-05、EZ15-08。田间试验结果表明,菌株EZ15-07和EZ01-05对玉米细菌性褐腐病的防治效果分别为59.3%和55.3%。对这2个菌株发酵液的MALDI-TOF-MS检测表明,菌株EZ15-07能产生Surfactin和Bacillomycin D 2种脂肽化合物,菌株EZ01-05能产生Bacillomycin D和Fengycin 2种脂肽化合物。[结论]由南极土壤分离获得的菌株EZ15-07和EZ01-05对玉米细菌性褐腐病防治效果显著,2个菌株均具有较好的田间应用潜力。
Abstract:
[Objectives]This study was conducted to select the Bacillus strains from the Antarctic soil,which had good biocontrol effect against brown stalk rot on maize. [Methods]The Bacillus strains were isolated from penguin rookery in the vicinity of the Great Wall Station of the Antarctic soil. Combining with 16S rDNA and gyrB gene sequence,the strains were identified. We selected the Bacillus strains,which had antagonism effect significantly against the pathogenic bacteria of the brown stalk rot on maize,by the plate antagonistic test. It was confirmed by the field trials that the Bacillus strains which were selected from the plate antagonistic test had good potential in application. The identification of antibacterial lipopeptide compounds were conducted with MALDI-TOF-MS. [Results]Combining with 16S rDNA and gyrB gene sequence analysis,the 23 strains of Bacillus which were isolated from the Antarctic soil were identified as Bacillus amyloliquefaciens(9 isolates)and Bacillus licheniformis(14 isolates). Through the plate antagonistic test,we got 4 strains which inhibited the pathogen of the brown stalk rot on maize significantly. They were B.amyloliquefaciens EZ15-07,EZ15-09,and B.licheniformis EZ01-05,EZ15-08. The results of the field trial showed that the biocontrol effect of EZ15-07 and EZ01-05 reached to 59.3% and 55.3%,respectively. The lipopeptide compounds analysis showed that strain EZ15-07 produced Surfactin and Bacillomycin D,whereas strain EZ01-05 produced Bacillomycin D and Fengycin. [Conclusions]The Bacillus strains EZ15-07 and EZ01-05 obtained from the Antarctic soil had significant biocontrol effects against brown stalk rot on maize. Both of them had good potential in application.

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备注/Memo

备注/Memo:
收稿日期:2016-11-07。
基金项目:国家公益性行业(农业)科研专项(20130315);国家高技术研究发展计划项目(2012AA101106);江苏省重点研发计划(现代农业)项目(BE2015354)
作者简介:朱碧春,硕士研究生。
通信作者:高学文,教授,博导,主要从事生防与细菌分子生物学研究,E-mail:gaoxw@njau.edu.cn。
更新日期/Last Update: 2017-07-07