[1]乔策策,王甜甜,王若斐,等.高效溶磷菌的筛选及其促生效应研究[J].南京农业大学学报,2017,(4):664-670.[doi:10.7685/jnau.201606030]
 QIAO Cece,WANG Tiantian,WANG Ruofei,et al.Screening phosphate solubilizing bacterial strains from maize rhizosphere and research on their plant growth promotion effect[J].Journal of Nanjing Agricultural University,2017,(4):664-670.[doi:10.7685/jnau.201606030]
点击复制

高效溶磷菌的筛选及其促生效应研究()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
期数:
2017年4期
页码:
664-670
栏目:
出版日期:
2017-07-07

文章信息/Info

Title:
Screening phosphate solubilizing bacterial strains from maize rhizosphere and research on their plant growth promotion effect
作者:
乔策策 王甜甜 王若斐 刘超 高琦 李荣 沈其荣
南京农业大学资源与环境科学学院/江苏省固体有机废弃物资源化高技术研究重点实验室/江苏省有机固体废弃物 资源化协同创新中心/国家有机类肥料工程技术研究中心, 江苏 南京 210095
Author(s):
QIAO Cece WANG Tiantian WANG Ruofei LIU Chao GAO Qi LI Rong SHEN Qirong
College of Resources and Environmental Sciences/Jiangsu Key Laboratory of Solid Organic Waste Utilization/Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization/National Engineering Research Center for Organic-based Fertilize
关键词:
溶磷菌溶磷量玉米促生增产
Keywords:
phosphate solubilizing bacteriaphosphate solubilizing amountmaizeplant growth promotioncrop yield increase
分类号:
S144.9
DOI:
10.7685/jnau.201606030
摘要:
[目的]从大田玉米根际土壤初筛溶磷能力较强的菌株,通过盆栽和大田试验复筛出具最优溶磷能力的玉米根际促生菌。[方法]利用改良的PVK培养基定性筛选溶磷菌株,通过NBRIP液体摇瓶试验定量研究其溶磷能力,再通过盆栽和大田试验来确定溶磷菌的促生和增产效果。[结果]从玉米大田根际土壤中筛选出8株具有解无机磷能力的细菌,其中菌株NJAU-B8在PVK培养基上溶磷圈直径与菌体直径的比值较高,在NBRIP液体培养基中有效磷含量最高达到487.67 mg·L-1。2季玉米盆栽试验结果表明,菌株NJAU-B8对苗期玉米生长促进作用优于其他菌株,其中第1季株高、茎粗、SPAD值、地上部鲜质量和地上部干质量较不接菌处理(CK)分别增加40.37%、18.74%、1.32%、46.67%和4.08%,而第2季则分别增加22.18%、2.02%、1.20%、16.26%和35.53%。另外除菌株B1,其他接菌处理株高均优于CK。大田试验结果表明:第1季玉米产量复合微生物肥料(BIO)与有机-无机复混肥(AOF)处理差异不显著,但BIO处理依然高于AOF和化肥(CF)处理;第2季白菜产量达到显著性差异;第3季BIO处理土豆产量分别比AOF和CF处理增产11.29%和26.41%。结合形态、生理生化特征和16S rDNA基因序列分析,将菌株NJAU-B8归类于伯克霍尔德属(Burkholderia sp.)。[结论]分离获得高效溶磷菌株NJAU-B8,其菌剂能够有效促进苗期玉米生长和增加玉米产量。
Abstract:
[Objectives]This study aimed to isolate phosphate-solubilizing bacterial strains from maize rhizosphere first,then to obtain one strain with best plant growth promotion ability from them though pot experiments and to evaluate its crop yield promotion effect using field experiments. [Methods]Modified PVK medium was used to screen phosphate-solubilizing strains qualitatively,then NBRIP liquid shake flask experiments were performed to evaluate the phosphate solubilizing ability of isolated strains,after that growth promotion and crop yield increasing effects of the strains were determined through pot and field experiments. [Results]From the rhizosphere soil of maize,eight bacterial strains with inorganic phosphorus-solubilizing ability were selected. 487.67 mg·L-1 of soluble phosphorus content was observed in NBRIP liquid culture medium inoculated with a strain named NJAU-B8 which showed higher ratio of transparent circle diameter to cell diameter. Pot experiment results showed a higher growth promoting effect of maize seedling was observed after inoculating strain NJAU-B8 than other strains and control. Compared to the control without inoculation,plant height,stem diameter,chlorophyll,fresh weight and dry weight of shoot were increased by 40.37%,18.74%,1.32%,46.67% and 4.08%,respectively,in the first season pot experiment and by 22.18%,2.02%,1.20%,16.26% and 35.53% in the second season pot experiment. Moreover,inoculation of other strains also showed higher plant height than the control except strain B1. No significant difference in the crop yield of maize was observed among different treatments in the first season field experiment,nevertheless,the yield in compound microbial fertilizer(BIO)treatment was higher compared to chemical fertilizer(CF)and organic-inorganic compound fertilizer(AOF)treatments;in the second field experiment,cabbage yield in BIO treatment was significantly higher than that in AOF and CF treatments;and in the third field experiment,potato yield in BIO treatment increased by 11.29% and 26.41% compared to AOF and CF,respectively. Combined with the morphological,physiological and biochemical characteristics and 16S rDNA gene sequence analysis,strain NJAU-B8 was classified into genus Burkholderia. [Conclusions]The isolated phosphate solubilizing bacterial strain NJAU-B8 can promote the growth of maize in the pots and increase crop yield in the field effectively.

参考文献/References:

[1] Tao G C,Tian S J,Cai M Y,et al. Phosphate-solubilizing and mineralizing abilities of bacteria isolated from soils[J]. Pedosphere,2008,18:515-523.
[2] Adesemoye A O,Torbert H A,Kloepper J W. Plant growth promoting rhizobacteria allow reduced application rates of chemical fertilizers[J]. Microbial Ecology,2009,58:921-929.
[3] 朱颖,姚拓,李玉娥,等. 红三叶根际溶磷菌分离及其溶磷机制初探[J]. 草地学报,2009,17(2):259-263.Zhu Y,Yao T,Li Y E,et al. Screening of phosphate-solubilizing bacteria and their acting mechanisms in the rhizosphere of Red Clover[J]. Acta Agrestia Sinica,2009,17(2):259-263(in Chinese with English abstract).
[4] 林启美,王华,赵小蓉,等. 一些细菌和真菌的解磷能力及其机理初探[J]. 微生物学通报,2001,28(2):26-30.Lin Q M,Wang H,Zhao X R,et al. Capacity of some bacteria and fungi in dissolving phosphate rock[J]. Microbiology China,2001,28(2):26-30(in Chinese with English abstract).
[5] Rudresh D L,Shivaprakash M K,Prasad R D. Effect of combined application of Rhizobium,phosphate solubilizing bacterium and Trichoderma spp. on growth,nutrient uptake and yield[J]. Applied Soil Ecology,2005,28:139-146.
[6] 陶涛,叶明,刘冬,等. 无机解磷细菌的筛选、鉴定及其溶磷能力研究[J]. 合肥工业大学学报(自然科学版),2011,34(2):304-308.Tao T,Ye M,Liu D,et al. On isolation and identification of inorganic Phosphbacteria and its phosphate-solubilizing capacity[J]. Journal of Hefei University of Technology(Natural Science Edition),2011,34(2):304-308(in Chinese with English abstract).
[7] Oliveira C A,Alves V M C,Marriel I E,et al. Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome[J]. Soil Biology and Biochemistry,2008,41(9):1782-1787.
[8] 胡晓峰. 溶磷菌的筛选、溶磷条件优化及对玉米的促生作用研究[D]. 南京:南京农业大学,2010.Hu X F. Screening of phosphate solubilizing bacteria optimization of phosphate dissolving conditions and ITS promotion to maize growth[D]. Nanjing:Nanjing Agricultural University,2010(in Chinese with English abstract).
[9] 戴沈艳,申卫收,贺云举,等. 一株高效解磷细菌的筛选及其在红壤性水稻土中的施用效果[J]. 应用与环境生物学报,2011,17(5):678-683.Dai S Y,Shen W S,He Y J,et al. Screening of efficient phosphate-solubilizing bacterial strain and its application in red paddy soil to rice cultivation[J]. Chinese Journal of Applied and Environmental Biology,2011,17(5):678-683(in Chinese with English abstract).
[10] Akhtar M S,Siddiqui Z A. Effects of phosphate solubilizing microorganisms and Rhizobium sp.on the growth,nodulation,yield and root-rot disease complex of chickpea under field condition[J]. African Journal of Biotechnology,2009,8:3489-3496.
[11] 中国科学院南京土壤研究所微生物室. 土壤微生物研究法[M]. 北京:科学出版社,1985:51.Microbilogy Lab,Institute of Soil Science,Chinese Academy of Sciences.Microorganism Research[M]. Beijing:Science Press,1985:51(in Chinese).
[12] Nautiyal C S. A method for selection and characterization of rhizosphere-competent bacteria of chickpea[J]. Current Microbiology,1997,34:12-17.
[13] 程宝森,房玉林,刘延琳,等. 渭北旱塬葡萄根际解磷细菌的筛选及其对葡萄的促生效应[J]. 西北农业学报,2009,18(4):185-190.Cheng B S,Fang Y L,Liu Y L,et al. Screening and promotion effect of phosphate-solubilizing bacteria from grapevine rhizosphere in Weibei semi-arid plateau[J]. Acta Agriculturae Boreali-Occidentalis Sinica,2009,18(4):185-190(in Chinese with English abstract).
[14] Yang C L,Li R,Song Y,et al. Identification of the biochemical degradation pathway of triazophos and its intermediate in Diaphorobacter sp. TPD-1[J]. Current Microbiology,2011,62:1294-1301.
[15] 肖春玲,宋勇生,王安萍,等. 菜园根际土壤解磷真菌的分离筛选及初步鉴定[J]. 井冈山大学学报(自然科学版),2012,33(4):48-51.Xiao C L,Song Y S,Wang A P,et al. Isolation and screening of phosphate-solubilizing fungi from the rhizospheric soil and its preliminary identification[J]. Journal of Jinggangshan University(Natural Science Edition),2012,33(4):48-51(in Chinese with English abstract).
[16] Glick B R. Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase[J]. FEMS Microbiology Letter,2005,251:1-7.
[17] 郜春花,卢朝东,张强.解磷菌剂对作物生长和土壤磷素的影响[J]. 水土保持学报,2006,20(4):54-56.Gao C H,Lu C D,Zhang Q. Solubilizing agent to the growth of crops and soil phosphorus element[J]. Journal of Soil and Water Conservation,2006,20(4):54-56(in Chinese with English abstract).
[18] 史国英,莫燕梅,岑贞陆. 一株高效解无机磷细菌BS06的鉴定及其解磷能力分析[J]. 微生物学通报,2015,42(7):1271-1278.Shi G Y,Mo Y M,Cen Z L. Identification of an inorganic phosphorus-dissolving bacterial strain BS06 and analysis on its phosphate solubilization ability[J]. Microbiology China,2015,42(7):1271-1278(in Chinese with English abstract).
[19] 申莉莉,王凤龙,钱玉梅,等. 解淀粉芽孢杆菌Ba33对烟草的促生及抗TMV作用[J]. 吉林农业大学学报,2010,32(4):383-386.Shen L L,Wang F L,Qian Y M,et al. Tobacco growth-promotion effect and TMV resistance of Bacillus amyloliquefaciens Ba33[J]. Journal of Jilin Agricultural University,2010,32(4):383-386(in Chinese with English abstract).
[20] 朱培淼,杨兴明,徐阳春,等. 高效解磷细菌的筛选及其对玉米苗期生长的促进作用[J]. 应用生态学报,2007,18(1):107-112.Zhu P M,Yang X M,Xu Y C,et al. High effective phosphate-solubilizing bacteria:their isolation and promoting effect on corn seedling growth[J]. Chinese Journal of Applied Ecology,2007,18(1):107-112(in Chinese with English abstract).
[21] 秦超琦,吴向华,郑琨,等. 解磷菌剂对海滨盐土有效磷含量及耐盐油料植物生长的影响[J]. 生态学杂志,2009,28(9):1835-1841.Qin C Q,Wu X H,Zheng K,et al. Effects of phosphate-solubilizing fungi on costalsaline soil available phosphorous content and salt-tolerant oil plant growth[J]. Chinese Journal of Ecology,2009,28(9):1835-1841(in Chinese with English abstract).
[22] 吴海燕.黑土磷素有效性的微生物调控技术及其机理研究[D]. 长春:吉林农业大学,2012:81-93.Wu H Y. Study on the microbial-control technique on phosphorus nutrient availability and its mechanisms in black soil[D]. Changchun:Jilin Agricultural University,2012:81-93(in Chinese with English abstract).
[23] 李晓举,吴风光,王豹祥,等. 一株烤烟根际解磷细菌的鉴定及其在烤烟生产中的应用[J]. 河南农业科学,2011,40(6):66-70.Li X J,Wu F G,Wang B X,et al. Identification and application of a phosphate solubilizing bacterium from flue-cured tobacco rhizosphere[J]. Journal of Henan Agricultural Sciences,2011,40(6):66-70(in Chinese with English abstract).
[24] 韩超,武贵元,刘爱新,等. 吡咯伯克霍尔德氏菌A12筛选鉴定及其对烟草幼苗的促生作用[J]. 浙江农业学报,2012,24(5):880-885.Han C,Wu G Y,Liu A X,et al. Screening and identification of the strain A12 and its effect on tobacco seedling growth[J]. Zhejiang Journal of Agricultural Science,2012,24(5):880-885(in Chinese with English abstract).
[25] 于晓庆,郗丽君,刘永光,等. 洋葱伯克霍尔德氏菌株Lyc2的鉴定及对棉苗的防病促生作用[J]. 植物病理学报,2007,37(4):426-432.Yu X Q,Xi L J,Liu Y G,et al. Identification of the onion holder strain Lyc2 and its effect on cotton seedling disease prevention and growth promoting[J]. Plant Pathology,2007,37(4):426-432(in Chinese with English abstract).

相似文献/References:

[1]张小兰,韦中,梅新兰,等.一种基于根际定殖能力筛选溶磷菌的方法[J].南京农业大学学报,2014,37(2):79.[doi:10.7685/j.issn.1000-2030.2014.02.013]
 ZHANG Xiaolan,WEI Zhong,MEI Xinlan,et al.A method for screening phosphate solubilizing bacteria based on the rhizosphere colonization ability of strains[J].Journal of Nanjing Agricultural University,2014,37(4):79.[doi:10.7685/j.issn.1000-2030.2014.02.013]
[2]冯哲叶,陈莎莎,王文超,等.几株溶磷细菌的筛选和鉴定及其溶磷效果[J].南京农业大学学报,2017,(5):842.[doi:10.7685/jnau.201612008]
 FENG Zheye,CHEN Shasha,WANG Wenchao,et al.Screening and identification of several phosphate-solubilizing bacteria and effect of their P-solubility[J].Journal of Nanjing Agricultural University,2017,(4):842.[doi:10.7685/jnau.201612008]

备注/Memo

备注/Memo:
收稿日期:2016-06-22。
基金项目:国家863计划项目(2013AA102802);中央高校基本科研业务费专项资金项目(KYZ201519);国家重点研发专项(2016YFD0800605,2016YFD0200106);江苏省农业科技自主创新资金项目[CX(15)100606];苏北专项(BN2015082);青蓝工程资助项目;南京农业大学SRT项目(1513A09);江苏省高校品牌专业建设工程资助项目(PPZY2015A061)
作者简介:乔策策,博士研究生。
通信作者:李荣
更新日期/Last Update: 2017-07-07