[1]王玮,滕爽,朱业培,等.动物过敏原牛血清白蛋白表面等离子共振传感器检测方法的建立[J].南京农业大学学报,2017,(4):739-743.[doi:10.7685/jnau.201612009]
 WANG Wei,TENG Shuang,ZHU Yepei,et al.Development of a surface plasmon resonance sensor for detection of animal allergen bovine serum albumin[J].Journal of Nanjing Agricultural University,2017,(4):739-743.[doi:10.7685/jnau.201612009]
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动物过敏原牛血清白蛋白表面等离子共振传感器检测方法的建立()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
期数:
2017年4期
页码:
739-743
栏目:
出版日期:
2017-07-07

文章信息/Info

Title:
Development of a surface plasmon resonance sensor for detection of animal allergen bovine serum albumin
作者:
王玮 滕爽 朱业培 徐幸莲 周光宏
南京农业大学国家肉品质量安全控制工程技术研究中心/农业部肉及肉制品质量监督检验测试中心(南京)/食品安全与营养协同创新中心, 江苏 南京 210095
Author(s):
WANG Wei TENG Shuang ZHU Yepei XU Xinglian ZHOU Guanghong
National Center of Meat Quality and Safety Control/Supervision, Inspection and Testing Center for Quality of Meat-products(Nanjing), Ministry of Agriculture/Synergetic Innovation Center of Food Safety and Nutrition, Nanjing Agricultural University, Nanjin
关键词:
牛血清白蛋白动物过敏原表面等离子共振检测
Keywords:
bovine serum albuminanimal allergensurface plasmon resonance(SPR)detection
分类号:
TS207.3
DOI:
10.7685/jnau.201612009
摘要:
[目的]建立一种表面等离子共振(surface plasmon resonance,SPR)技术,用于快速、准确、灵敏、定量检测动物过敏原牛血清白蛋白(bovine serum albumin,BSA)。[方法]将鼠抗BSA单克隆抗体偶联于SPR生物传感器芯片表面,监测BSA与抗体结合后芯片表面的变化,并基于SPR技术建立快速检测微量BSA的新方法,确定其检出限和最佳线性范围,并进行重复性验证和初步应用。[结果]抗体的偶联水平为18 000,对BSA具有良好的亲和能力;BSA质量浓度在6.25~400 ng·;mL-1范围内,与SPR响应值的变化量呈良好的线性关系(R2=0.992),方法检测限为33.68 ng·;mL-1;用建立的SPR方法检测不同浓度BSA的日内RSD为1.12%~3.42%,日间RSD为0.84%~3.98%,均小于5%;SPR方法与ELISA商业化试剂盒检测不同市售食品中BSA含量的结果比较,差异不显著(P>0.05)。[结论]SPR方法可靠性好、稳定性强,适用于食品中过敏原BSA的高通量实时检测。
Abstract:
[Objectives]A rapid,accurate and sensitive method for the quantitative detection of animal allergen bovine serum albumin(BSA)was developed using surface plasmon resonance(SPR)technology. [Methods]SPR was developed by monitoring the interaction between BSA and a self-prepared mouse anti-BSA monoclonal antibody on the SPR sensor chip,then determined for limit of detection(LOD)and optimal linear range,verified for reproducibility and applied preliminarily. [Results]The specific monoclonal antibody against BSA at a labeling level of 18 000 showed good affinity to BSA. The BSA concentration at a range of 6.25-400 ng·mL-1 showed good linear relationship to the response value of SPR analyzer(R2=0.992),with LOD of 33.68 ng·mL-1. The RSD of intra- and inter-assays on BSA at various concentrations by the developed SPR method was 1.12%-3.42% and 0.84%-3.98%,respectively,both of which were less than 5%. No significant differences were observed between the determination results of BSA of commercial meat and meat products by SPR and ELISA methods(P>0.05). [Conclusions]The SPR method constructed in the paper has high reliability and stability,and can be used for high-throughput and real-time detection of animal allergen BSA in food.

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相似文献/References:

[1]朱业培,王玮,吕青骎,等.动物过敏原牛血清白蛋白间接竞争ELISA检测方法的建立[J].南京农业大学学报,2016,39(2):305.[doi:10.7685/jnau.20150814]
 ZHU Yepei,WANG Wei,L Qingqin,et al.Development of an indirect competitive ELISA for detection of animal allergen bovine serum albumin[J].Journal of Nanjing Agricultural University,2016,39(4):305.[doi:10.7685/jnau.20150814]

备注/Memo

备注/Memo:
收稿日期:2016-12-08。
基金项目:国家自然科学基金项目(31501395);江苏省自然科学基金项目(BK20140722)
作者简介:王玮,副教授,主要从事食品生物安全快速检测与控制技术研究,E-mail:wangwei821220@njau.edu.cn。
更新日期/Last Update: 2017-07-07