[1]贾坤,廖燕红,徐磊,等.酰基-高丝氨酸内酯(AHL)对鼠伤寒沙门氏菌生物菌膜形成的影响[J].南京农业大学学报,2017,(4):744-749.[doi:10.7685/jnau.201611023]
 JIA Kun,LIAO Yanhong,XU Lei,et al.Effect of acylated homoserine lactones(AHL)on the biofilm formation of Salmonella typhimurium[J].Journal of Nanjing Agricultural University,2017,(4):744-749.[doi:10.7685/jnau.201611023]
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酰基-高丝氨酸内酯(AHL)对鼠伤寒沙门氏菌生物菌膜形成的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
期数:
2017年4期
页码:
744-749
栏目:
出版日期:
2017-07-07

文章信息/Info

Title:
Effect of acylated homoserine lactones(AHL)on the biofilm formation of Salmonella typhimurium
作者:
贾坤 廖燕红 徐磊 王虎虎 徐幸莲
南京农业大学国家肉品质量安全控制工程技术研究中心/江苏省肉类生产 与加工质量安全控制协同创新中心, 江苏 南京 210095
Author(s):
JIA Kun LIAO Yanhong XU Lei WANG Huhu XU Xinglian
National Center of Meat Quality and Safety Control/Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing 210095, China
关键词:
酰基-高丝氨酸内酯(AHL)铜绿假单胞菌鼠伤寒沙门氏菌生物菌膜形成
Keywords:
acylated homoserine lactones(AHL)Pseudomonas aeruginosaSalmonella typhimuriumbiofilm formation
分类号:
Q935
DOI:
10.7685/jnau.201611023
摘要:
[目的]研究铜绿假单胞菌所产生的群体感应信号分子酰基-高丝氨酸内酯(AHL)对鼠伤寒沙门氏菌生物菌膜形成的影响,为不同菌体间群体感应的研究提供参考,为沙门氏菌的控制提供帮助。[方法]利用薄层层析法分析铜绿假单胞菌所产生AHL的成分,并通过添加0%、1.0%、1.5%、2.0%(体积分数)的AHL来研究其对鼠伤寒沙门氏菌生物菌膜形成、浮游菌体生长以及菌体泳动能力的影响。[结果]铜绿假单胞菌产生2种相同类型的AHL分子(C12-HSL和3-oxo-C8-HSL),100 ℃加热5 min并不会改变AHL的成分。当AHL添加量低于1.5%时,虽然浮游菌体指数生长时期受到一定抑制,但随着时间的延长,稳定期浮游菌体的生长并无显著差异,反观生物菌膜,其形成明显受到抑制。当AHL添加量达到2%时,浮游菌体生长和生物菌膜形成均受到显著抑制,说明AHL对鼠伤寒沙门氏菌生物菌膜的形成有明显抑制作用。AHL对单一菌体泳动性有显著抑制作用,而对群体泳动性有不同程度的促进作用。[结论]群体感应信号分子AHL对鼠伤寒沙门氏菌浮游菌体生长的影响受添加量调控,当AHL添加量增加到一定值时浮游菌体生长才受到抑制,但对生物菌膜的形成有明显抑制作用,且随着AHL添加量提高,抑制作用增强。
Abstract:
[Objectives]This study investigated the effect of quorum-sensing molecules acylated homoserine lactones(AHL)from Pseudomonas aeruginosa on the biofilm formation of Salmonella typhimurium,which provided reference for further study of quorum-sensing of different bacteria and help for the control of Salmonella. [Methods]The components of AHL from P.aeruginosa was studied by the thin-layer chromatography(TLC). The effect of AHL on the biofilm formation,plankton and motility of S.typhimurium was studied by adding different concentrations of AHL(0%,1.0%,1.5%,2.0%). [Results]P.aeruginosa can produce two same kinds of AHL(C12-HSL and 3-oxo-C8-HSL)and heating at 100 ℃for 5 min had no effect on the components of AHL. When the AHL concentration was below 1.5%,the exponential growth period of planktonic bacteria was inhibited,the stationary phase was not significantly affected,and the biofilm formation of S.typhimurium was significantly inhibited. However,both the growth of planktonic bacteria and the biofilm formation were significantly inhibited by adding 2% AHL,indicating that AHL had significant inhibition on the biofilm formation of S.typhimurium. The swimming of S.typhimurium was significantly inhibited by the presence of AHL. Conversely,the swarming was promoted by the presence of AHL. [Conclusions]The effect of quorum-sensing molecules(AHL)on the growth of planktonic bacterial depended on the concentrations,and the planktonic bacterial was inhibited only when the concentration of AHL reached a certain value. But the presence of AHL had significant inhibition of the biofilm formation of S.typhimurium,and the inhibition was increased with the increased AHL concentration.

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备注/Memo

备注/Memo:
收稿日期:2016-11-22。
基金项目:江苏省科技计划青年基金项目(BK20150678);国家自然基金青年基金项目(31501574)
作者简介:贾坤,硕士研究生。
通信作者:王虎虎,博士,讲师,主要从事肉品质量与安全控制研究,E-mail:huuwang@njau.edu.cn。
更新日期/Last Update: 2017-07-07