[1]朱怀森,钱庄,杨东风,等.注射ACTH对断奶母猪卵泡促黄体素受体表达及卵泡液中类固醇激素合成的影响[J].南京农业大学学报,2017,40(6):1081-1087.[doi:10.7685/jnau.201608010]
　ZHU Huaisen,QIAN Zhuang,YANG Dongfeng,et al.The effect of ACTH injection on follicular fluid concentrations of steroid hormones and expressions of luteinizing hormone receptors in weaned Suhuai sows[J].Journal of Nanjing Agricultural University,2017,40(6):1081-1087.[doi:10.7685/jnau.201608010]
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注射ACTH对断奶母猪卵泡促黄体素受体表达及卵泡液中类固醇激素合成的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
40卷
期数:
2017年6期
页码:
1081-1087
栏目:
出版日期:
2017-11-10

文章信息/Info

Title:
The effect of ACTH injection on follicular fluid concentrations of steroid hormones and expressions of luteinizing hormone receptors in weaned Suhuai sows
作者:
朱怀森1;  钱庄1;  杨东风2;  刘洪林3;  鲍恩东1
1. 南京农业大学动物医学院, 江苏 南京 210095;
2. 宁夏农牧厅农业国际合作项目管理中心, 宁夏 银川 750002;
3. 南京农业大学动物科技学院, 江苏 南京 210095
Author(s):
ZHU Huaisen1;  QIAN Zhuang1;  YANG Dongfeng2;  LIU Honglin3;  BAO Endong1
1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2. International Agricultural Cooperation Project Management Center of Ningxia Department of Agricultural and Animal Husbandry, Yinchuan 750002, China;
3. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
关键词:
促肾上腺皮质激素(ACTH); 类固醇激素; 促黄体素受体(LHR); 应激; 断奶母猪
Keywords:
adrenocorticotropic hormone(ACTH); steroid hormone; luteinizing hormone receptor(LHR); stress; weaned sow
分类号:
S814.1
DOI:
10.7685/jnau.201608010
摘要:
[目的] 为了探讨应激对断奶母猪繁殖障碍的影响机制，本试验以促肾上腺皮质激素（ACTH）注射所致的应激模型来研究应激对断奶母猪卵泡液中类固醇激素合成及卵泡促黄体素受体表达的影响。[方法] 对10头苏淮断奶母猪进行颈静脉插管手术，随机均分为对照组与ACTH处理组（n=5）。手术第3天开始以每次间隔8 h给ACTH处理组母猪注射ACTH （1 IU·kg-1），对照组母猪注射生理盐水，连续注射7 d。利用ELISA方法检测受试母猪卵泡液中类固醇激素及血浆中皮质醇的含量变化；利用荧光定量PCR测定胆固醇侧链裂解酶（CYP11A1）、17α-羟化酶（CYP17A1）、芳香化酶（CYP19A1）、类固醇激素合成急性期调节蛋白（StAR）、3β羟基类固醇脱氢酶（3β-HSD）、促黄体素受体（LHR）等基因的表达；利用免疫组化法检测CYP17A1、CYP19A1、LHR和3β-HSD蛋白的表达。[结果] 人工注射ACTH后40%的受试母猪出现排卵障碍；其血浆中皮质醇的含量极显著升高（P<0.01）；母猪卵泡液中雌二醇、雄烯二酮含量均显著下降（P<0.05）；CYP17A1、CYP19A1、LHR和3β-HSD基因表达量降低，其编码的蛋白CYP17A1、CYP19A1、LHR和3β-HSD表达量也明显下降。[结论] ACTH注射所致应激过程中，受试母猪的皮质醇含量升高可导致类固醇合成酶的基因、蛋白表达量明显下降，从而影响类固醇激素的合成及卵泡的发育。类固醇激素合成降低及LHR的低表达影响断奶母猪的正常排卵。
Abstract:
[Objectives] To investigate the mechanism of ovulation failure caused by stress in weaned sows, the effects of adrenocorticotropic hormone (ACTH)injection on the steroidogenesis capacity in follicular fluid and the expression of luteinizing hormone receptor (LHR)in follicular wall were studied in weaned sows.[Methods] All ten tested sows (n=10)were fitted with jugular vein catheters on the weaning day and randomly divided into control group and ACTH treatment group (n=5). Three days after surgery, the sows were injected with 1 IU·kg-1 ACTH or 9 g·L-1 NaCl every 8 h and continued for 7 days. The follicular fluid concentrations of steroid hormones and plasma concentrations of cortisol were determined using ELISA kits. The mRNA translation levels of cytochrome P450 side-chain cleavage (CYP11A1), cytochrome P450 17α-hydroxylase (CYP17A1), cytochrome P450 aromatase (CYP19A1), steroidogenic acute regulatory protein (StAR), 3β-hydroxysteroid dehydrogenase (3β-HSD)and LHR were quantified by real-time quantitative PCR. The protein expressions of CYP17A1, CYP19A1, LHR and 3β-HSD were detected by immunohistochemistry.[Results] The results showed that 40% of the tested sows displayed ovulation failure after ACTH injection. Plasma concentrations of cortisol significantly increased (P<0.01)and follicular fluid concentrations of estradiol and androstenedione significantly were reduced (P<0.05). The transcriptional levels of CYP17A1, CYP19A1, LHR and 3β-HSD were reduced (P<0.05)and their corresponding protein expressions of CYP17A1, CYP19A1, LHR and 3β-HSD also decreased (P<0.05).[Conclusions] The results suggest that, during the process of stress induced by ACTH injection, the decline of genes and their corresponding proteins expressions of steroid synthesisases reduced by inductions of cortisol in plasma will influence the steroid hormone synthesis and follicle development. The normal ovulation of weaned sows will be influenced by the lower levels of steroid hormones and the lower expression of LHR.

参考文献/References:

[1] Einarsson S,Brandt Y,Lundeheim N,et al. Stress and its influence on reproduction in pigs:a review[J]. Acta Vet Scand,2008,50:48.
[2] Dobson H,Smith R F. What is stress,and how does it affect reproduction?[J]. Animal Reproduction Science,2000,60/61:743-752.
[3] Vargas A J,Bernardi M L,Paranhos T F,et al. Reproductive performance of swine females re-serviced after return to estrus or abortion[J]. Anim Reprod Sci,2009,113(1/2/3/4):305-310.
[4] Hennessy D P,Williamson P. The effects of stress and of ACTH administration in hormone profiles,oestrus and ovulation in pigs[J]. Theriogenology,1983,20(1):13-26.
[5] Szulanczyk-Mencel K,Rzasa A,Bielas W. Relationships between ovarian cysts and morphological and hormonal state of ovarian cortex in sows[J]. Anim Reprod Sci,2010,121(3/4):273-278.
[6] Madej A,Lang A,Brandt Y,et al. Factors regulating ovarian function in pigs[J]. Domestic Animal Endocrinology,2005,29(2):347-361.
[7] 林鹏飞,剧世强,罗碧平,等. 猪有腔卵泡发育和闭锁中颗粒细胞凋亡与卵泡液类固醇激素的变化[J]. 西北农林科技大学学报(自然科学版),2011,39(3):28-33,39. Lin P F,Ju S Q,Luo B P,et al. Analysis of the granulosa cells apoptosis and the changes of hormone during porcine follicular development and atresia[J]. Journal of Northwest A&F University(Natural Science Edition),2011,39(3):28-33,39(in Chinese with English abstract).
[8] Scholten J A,Liptrap R M. A role for the adrenal cortex in the onset of cystic ovarian follicles in the sow[J]. Can J Comp Med,1978,42(4):525-533.
[9] Barb C R,Kraeling R R,Rampacek G B,et al. Inhibition of ovulation and LH secretion in the gilt after treatment with ACTH or hydrocortisone[J]. J Reprod Fertil,1982,64(1):85-92.
[10] 孙嘉瑞,曹轶男,张新晨,等. 三聚氰胺与三聚氰酸混合物对小鼠睾酮生成和调控的影响[J]. 南京农业大学学报,2016,39(4):650-655. DOI:10.7685/jnau.201510037. Sun J R,Cao Y N,Zhang X C,et al. Effect of mixture of melamine and cyanuric acid on production and synthesis regulation of testosterone in mice[J]. Journal of Nanjing Agricultural University,2016,39(4):650-655(in Chinese with English abstract).
[11] Nimz M,Spitschak M,Schneider F,et al. Down-regulation of genes encoding steroidogenic enzymes and hormone receptors in late preovulatory follicles of the cow coincides with an accumulation of intrafollicular steroids[J]. Domestic Animal Endocrinology,2009,37(1):45-54.
[12] 张金璧,林飞,潘增祥,等. 猪不同程度闭锁卵泡判定方法的比较研究[J]. 南京农业大学学报,2013,36(1):115-119. DOI:10.7685/j.issn.1000-2030.2013.01.020 Zhang J B,Lin F,Pan Z X,et al. Comparative study of methods to determine the follicular atresia extent in pigs[J]. Journal of Nanjing Agricultural University,2013,36(1):115-119(in Chinese with English abstract).
[13] Sapolsky R M. How do glucocorticoids influence stress responses?Integrating permissive,suppressive,stimulatory,and preparative actions[J]. Endocrine Reviews,2000,21(1):55-89.
[14] 付灵梅,谭朝阳,王丽君,等. 紫石英对排卵障碍大鼠卵巢局部卵泡刺激素受体、黄体生成素受体表达的影响[J]. 中国实验方剂学杂志,2011,19(5):184-186. Fu L M,Tan C Y,Wang L J,et al. Influence of fluoritum on rats with ovarian failure and level of FSHR and LHR[J]. Chinese Journal of Experimental Traditional Medical Formulate,2011,19(5):184-186(in Chinese with English abstract).

备注/Memo

备注/Memo:
收稿日期:2016-08-08。
基金项目:国家973计划项目（2014CB138502）
作者简介:朱怀森,硕士研究生。
通信作者:鲍恩东,教授,主要从事兽医病理学及免疫病理学研究,E-mail:b_endong@njau.edu.cn。
更新日期/Last Update: 1900-01-01