SHI Lichao,ZENG Aisong,LI Jiayi,et al.Studies on characteristics of a heart-shaped glossy cabbage mutants 410M[J].Journal of Nanjing Agricultural University,2018,41(1):57-63.[doi:10.7685/jnau.201705001]





Studies on characteristics of a heart-shaped glossy cabbage mutants 410M
石利朝12 曾爱松2 李家仪3 王金香12 宋立晓2 严继勇2
1. 南京农业大学园艺学院, 江苏 南京 210095;
2. 江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室, 江苏 南京 210014;
3. 中国农业大学生物学院, 北京 100083
SHI Lichao12 ZENG Aisong2 LI Jiayi3 WANG Jinxiang12 SONG Lixiao2 YAN Jiyong2
1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China;
3. College of Biological Sciences, China Agricultural University, Beijing 100083, China
cabbageglossy mutantagronomic traitultrastructureinheritancewax genes
[Objectives]To enrich the germplasm resources and promote the improvement of new varieties with desirable traits of cabbage,a heart-shaped glossy mutant 410M of cabbage was studied. [Methods]The major nutritional components and phenotypic characterization of 410M plants were analyzed,and the wax amount and crystal structure were observed by scanning electron micrographs(SEM). Then,to study the inheritance of glossy wax-less characteristic,F1,F2,BC1P1 and BC1P2 populations were obtained through crossing,selfing and back-crossing by 410M(mutant) and 410W(wild) plants. Lastly,the transcriptional profiles of 14 genes that were related to wax biosynthesis and transport in Arabidopsis were analyzed by real-time quantitative RT-PCR. [Results]410M plants had a remarkable glossy surface phenotypic trait throughout their growth and development,and other phenotypes were indistinguishable from those of 410W. There were no significant differences in content of the major nutritional components including protein,crude fiber,total sugar and dry matter between 410M and 410W except vitamin C content. The SEM analysis revealed a significant difference in the total wax amount between 410M and 410W plants,and the surface of mature leaves of 410W was covered by dense linear or columnar wax crystals on both the ventral and abaxial of the leaf blade. Conversely,the mature leave surface of 410M was almost devoid of wax crystals,with only a small portion of granule-like wax crystals present on the smooth wax film. Then,the F1s indicated that non-glossy leaves were dominant over glossy leaves. The genetic ratio of 3 non-glossy to 1 glossy was obtained in F2 generations,and the back-cross generation involving a glossy parent as the recurrent parent showed a ratio of 1 non-glossy to 1 glossy. So,the results ofχ2 test on the separated proportion proved that the trait of glossy wax-less was controlled by one pair of recessive gene. Lastly,our work also showed that some genes that were involved in wax biosynthesis,and export pathways were down-regulated in 410M,which could cause the glossy phenotype of mutant plants. [Conclusions]410M exhibited excellent comprehensive properties with remarkable and stable glossy phenotype,and belongs to a core heart-shaped parent for future cabbage breeding programs and molecular studies.


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更新日期/Last Update: 1900-01-01