[1]潘俏,张舒玥,沈迪,等.黄瓜耐盐突变体材料的筛选与鉴定[J].南京农业大学学报,2018,41(1):64-70.[doi:10.7685/jnau.201704010]
 PAN Qiao,ZHANG Shuyue,SHEN Di,et al.Screening and identification of salt tolerant mutants of cucumber[J].Journal of Nanjing Agricultural University,2018,41(1):64-70.[doi:10.7685/jnau.201704010]
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黄瓜耐盐突变体材料的筛选与鉴定()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年1期
页码:
64-70
栏目:
出版日期:
2018-01-15

文章信息/Info

Title:
Screening and identification of salt tolerant mutants of cucumber
作者:
潘俏 张舒玥 沈迪 吴家琪 陈春莲 陈洁 陈劲枫 娄群峰
南京农业大学作物遗传与种质创新国家重点实验室/园艺学院, 江苏 南京 210095
Author(s):
PAN Qiao ZHANG Shuyue SHEN Di WU Jiaqi CHEN Chunlian CHEN Jie CHEN Jinfeng LOU Qunfeng
State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
黄瓜突变体耐盐性筛选鉴定
Keywords:
cucumbermutantssalt tolerancescreening and identification
分类号:
S642.2
DOI:
10.7685/jnau.201704010
摘要:
[目的]本文旨在从黄瓜突变体材料中筛选耐盐突变体材料。[方法]对948份’长春密刺’黄瓜突变体材料进行NaCl溶液催芽试验,通过对比发芽率、发芽势和相对发芽率,结合胚根生长状况进行初步筛选,然后对发芽期筛选出的优质材料进行幼苗期盐胁迫处理(120 mmol·L-1NaCl营养液,处理7 d),测定株高、根长、茎粗、地上部鲜质量、地下部鲜质量、茎节数等形态指标,分析超氧化物歧化酶、过氧化物酶、过氧化氢酶活性以及叶绿素a、叶绿素b、类胡萝卜素和可溶性蛋白含量等生理指标。[结果]根据发芽期突变体材料的发芽指标情况,共筛选出25份耐盐突变体材料,从中挑选出12份优质材料。在盐胁迫处理下12份黄瓜突变体各项形态及生理指标均出现下降,但耐盐性好的材料表现显著优于对照,最终经过综合比较后筛选出7份耐盐新材料:Mu-94-6、Mu-34-2、Mu-144-10、Mu-70-11、Mu-42-5、Mu-61-2、Mu-43-4。[结论]通过对突变体库的筛选,得到7份耐盐新材料。这些材料的获得对黄瓜耐盐性的遗传研究和新品种培育具有重要意义。
Abstract:
[Objectives]This article aims to screen the salt tolerant materials of cucumber. [Methods]Total 948 ‘Changchunmici’ cucumber mutants were subjected to NaCl solution germination test,and salt tolerant lines were preliminarily selected by comparing germination rate,germination potential,relative germination rate,and radicle growth status. Further,We treated these lines with nutrient solution containing NaCl with a concentration of 120 mmol·L-1 for 7 days at seedling stage. And then the morphological indicators(plant height,root length,stem diameter,fresh weight on the ground,fresh weight of the ground and stem number) and physiological indexes(SOD,POD and CAT activities,chlorophyll a,chlorophyll b,carotenoid and soluble protein contents) were investigated. [Results]25 candidate salt tolerant lines were preliminarily selected by comparing the germination index. Further,12 materials were selected from these lines and treated with nutrient solution containing NaCl. Salt stress caused morphological and physiological indexes of cucumber to decline,but theses indexes in salt tolerant materials of cucumber were significantly higher than them in the control. Based on these results,7 salt-tolerant new materials(Mu-94-6,Mu-34-2 and Mu-144-10,Mu-70-11,Mu-42-5,Mu-61-2 and Mu-43-4) with high tolerance to salt were finally identified. [Conclusions]Through the screening of mutants,7 salt-tolerant new materials were obtained. These materials are of great significance and value to the genetic research and cultivation of new varieties of cucumber.

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备注/Memo

备注/Memo:
收稿日期:2017-04-07。
基金项目:国家"十三五"重点研发计划子课题(2016YFD0100204-25);江苏省农业科技自主创新项目[CX(17)3016];江苏高校"青蓝工程"人才项目
作者简介:潘俏,硕士研究生。
通信作者:娄群峰,教授,从事葫芦科作物遗传育种与种质创新研究,E-mail:qflou@njau.edu.cn。
更新日期/Last Update: 1900-01-01