FU Yuanyuan,CHEN Kunlin,WANG Zhenyun,et al.Lipolysis mechanism of Chemerin in bovine intramuscular mature adipocytes[J].Journal of Nanjing Agricultural University,2018,41(1):120-125.[doi:10.7685/jnau.201703100]





Lipolysis mechanism of Chemerin in bovine intramuscular mature adipocytes
付园园1 陈坤琳1 王振云1 周光宏2 李惠侠1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 南京农业大学肉品加工与质量控制教育部重点实验室, 江苏 南京 210095
FU Yuanyuan1 CHEN Kunlin1 WANG Zhenyun1 ZHOU Guanghong2 LI Huixia1
1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. Key Laboratory of Meat Products Processing and Quality Control, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China
Chemerinintramuscular mature adipocyteslipolysis mechanismbovine
[目的]本试验旨在分析脂肪自分泌因子Chemerin在牛肌内成熟脂肪细胞脂解中的作用及调控机制。[方法]体外培养牛肌内成熟脂肪细胞,采用油红O染色与抽提法检测成熟脂肪细胞脂解过程中脂肪含量变化,通过比色法测定细胞中甘油及游离脂肪酸的释放量;采用RT-qPCR及Western blot技术分析脂解关键基因PPARα、LPLHSL mRNA及蛋白的表达情况。[结果]在牛肌内成熟脂肪细胞脂解过程中,细胞中Chemerin及其受体CMKLR1 mRNA表达量显著降低(P<0.05);Chemerin处理牛肌内成熟脂肪细胞后,细胞内脂肪含量减少,成熟脂肪细胞快速脂解为去分化的前体脂肪细胞,细胞中甘油和游离脂肪酸的释放量增加。脂解关键基因PPARα、LPLHSL mRNA及蛋白表达水平与对照组相比极显著升高(P<0.01)。[结论]Chemerin通过上调脂肪细胞脂解关键基因表达,促进了牛肌内成熟脂肪细胞中脂肪的分解,进而可降低肌内脂肪含量。
[Objectives]The aim of this study is to investigate effects and molecular mechamisms of Chemerin in the lipolysis of bovine intramuscular mature adipocytes. [Methods]Bovine intramuscular mature adipocytes were cultured and treated by Chemerin in vitro,and fat content and cell morphological change of the mature adipocyte in the process of lipolysis were measured by oil red O staining. The release of glycerol and free fatty acids was measured by colorimetry,and mRNA and protein expression of key lipolytic genes such as peroxisome proliferator-activated receptor α(PPARα),lipoprotein lipase(LPL) and hormone-sensitive lipase(HSL) were measured by real-time quantitative PCR(RT-qPCR) and Western blot. [Results]mRNA expression of Chemerin and CMKLR1 were significantly decreased in the process of lipolysis(P<0.05). Moreover,in the Chemerin-treatment group,there are less lipid droplets in cells,and the fat in mature adipocytes was accelerated to decompose into glycerol and fatty acid. Mature adipocytes de-differentiate into fibroblast-like pre-adipocytes faster than in control cells. In addition,mRNA and protein expression of PPARα,LPL and HSL were significantly up-regulated by Chemerin during mature adipocytes steatolysis compared to control group(P<0.01). [Conclusions]Through up-regulation the expression of key lipolysis genes,Chemerin promoted the decomposition of fat in bovine intramuscular mature adipocytes,which can reduce the content of intramuscular fat.


[1] Troy D J,Tiwari B K,Joo S T. Health implications of beef intramuscular fat consumption[J]. Korean Journal for Food Science of Animal Resources,2016,36(5):577-582.
[2] 孙文星,南文婷,谷淑华,等. miR-27b及其靶基因PPARγ在肌内和皮下脂肪细胞中的差异表达分析[J]. 南京农业大学学报,2016,39(2):249-254. DOI:10.7685/jnau.201505022 Sun W X,Nan W T,Gu S H,et al. Differential expression of miR-27b and its target gene PPARγ in intramuscular and subcutaneous adipocytes[J]. Journal of Nanjing Agricultural University,2016,39(2):249-254(in Chinese with English abstract).
[3] Pannier L,Sweeney T,Hamill R M,et al. Lack of an association between single nucleotide polymorphisms in the bovine leptin gene and intramuscular fat in Bos taurus cattle[J]. Meat Science,2009,81(4):731-737.
[4] Bozaoglu K,Bolton K,McMillan J,et al. Chemerin is a novel adipokine associated with obesity and metabolic syndrome[J]. Endocrinology,2007,148(10):4687-4694.
[5] Cash J L,Hart R,Russ A,et al. Synthetic Chemerin-derived peptides suppress inflammation through ChemR23[J]. The Journal of Experimental Medicine,2008,205(4):767-775.
[6] Roh S G,Song S H,Choi K C,et al. Chemerin:a new adipokine that modulates adipogenesis via its own receptor[J]. Biochemical and Biophysical Research Communications,2007,362(4):1013-1018.
[7] Roman A A,Parlee S D,Sinal C J. Chemerin:a potential endocrine link between obesity and type 2 diabetes[J]. Endocrine,2012,42(2):243-251.
[8] Ernst M C,Sinal C J. Chemerin:at the crossroads of inflammation and obesity[J]. Trends in Endocrinology and Metabolism,2010,21(11):660-667.
[9] Wolfgang M J,Cha S H,Sidhaye A,et al. Regulation of hypothalamic malonyl-CoA by central glucose and leptin[J]. Proc Natl Acad Sci USA,2007,104(49):19285-19290.
[10] Zhao S,Wang J,Song X,et al. Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue[J]. Nutrition and Metabolism,2010,7(6):3-13.
[11] Cwiklinska A,Gliwinska A,Senderowska Z,et al. Impact of phosphatidylcholine liposomes on the compositional changes of VLDL during lipoprotein lipase(LPL) -mediated lipolysis[J]. Chemistry and Physics of Lipids,2016,195:63-70.
[12] Zimmermann R,Strauss J G,Haemmerle G,et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase[J]. Science,2004,306(5700):1383-1386.
[13] 李惠侠,罗肖,刘荣鑫,等. 用成熟脂肪建立一种新的猪前体脂肪细胞培养模型[J]. 中国生物化学与分子生物学报,2009,25(3):270-275. Li H X,Luo X,Liu R X,et al. Novel preadipocyte cell model estabolished via pig mature adipocytes[J]. Chinese Journal of Biochemistry and Molecular Biology,2009,25(3):270-275(in Chinese with English abstract).
[14] Yoshimura T,Oppenheim J J. Chemokine-like receptor 1(CMKLR1) and chemokine(C-C motif) receptor-like 2(CCRL2),two multifunctional receptors with unusual properties[J]. Experimental Cell Research,2011,317(5):674-684.
[15] Goralski K B,McCarthy T C,Hanniman E A,et al. Chemerin,a novel adipokine that regulates adipogenesis and adipocyte metabolism[J]. The Journal of Biological Chemistry,2007,282(38):28175-28188.
[16] Sell H,Laurencikiene J,Taube A,et al. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells[J]. Diabetes,2009,58(12):2731-2740.
[17] 胡越,许军,刘燕华,等. PPARα激动剂降血脂作用的研究进展[J]. 中国药科大学学报,2016,47(1):118-124. Hu Y,Xu J,Liu Y H,et al. Advances in lipid-lowering effect of PPARα agonists[J]. Journal of China Pharmaceutical University,2016,47(1):118-124(in Chinese with English abstract).
[18] Fruchart J C. Peroxisome proliferator-activated receptor-alpha(PPARalpha):at the crossroads of obesity,diabetes and cardiovascular disease[J]. Atherosclerosis,2009,205(1):1-8.
[19] Duez H,Lefebvre B,Poulain P,et al. Regulation of human apoA-I by gemfibrozil and fenofibrate through selective peroxisome proliferator-activated receptor alpha modulation[J]. Arteriosclerosis,Thrombosis,and Vascular Biology,2005,25(3):585-591.
[20] Berman D M,Nicklas B J,Ryan A S,et al. Regulation of lipolysis and lipoprotein lipase after weight loss in obese,postmenopausal women[J]. Obesity Research,2004,12(1):32-39.
[21] 姜延志,邢淑华,岑王敏,等. 脂蛋白脂酶调控因子研究进展[J]. 遗传,2013,35(7):830-838. Jiang Y Z,Xing S H,Cen W M,et al. New insights in regulation factors of lipoprotein lipase[J]. Hereditas,2013,35(7):830-838(in Chinese with English abstract).
[22] Nielsen T S,Jessen N,Jorgensen J O,et al. Dissecting adipose tissue lipolysis:molecular regulation and implications for metabolic disease[J]. Journal of Molecular Endocrinology,2014,52(3):199-222.


 DUAN Yinping,XUE Ying,ZHOU Shuang,et al.Effect of Chemerin on apoptosis and autophagy in bovine granulosa cells[J].Journal of Nanjing Agricultural University,2019,42(1):145.[doi:10.7685/jnau.201801017]


更新日期/Last Update: 1900-01-01