[1]郭阳鑫,卫志明,佘跃辉,等.大豆叶柄离体再生体系研究[J].南京农业大学学报,2018,41(1):190-194.[doi:10.7685/jnau.201703035]
 GUO Yangxin,WEI Zhiming,SHE Yuehui,et al.Research on plantlet regeneration from petiole explants in soybean[J].Journal of Nanjing Agricultural University,2018,41(1):190-194.[doi:10.7685/jnau.201703035]
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大豆叶柄离体再生体系研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年1期
页码:
190-194
栏目:
出版日期:
2018-01-15

文章信息/Info

Title:
Research on plantlet regeneration from petiole explants in soybean
作者:
郭阳鑫123 卫志明2 佘跃辉1 朱木兰23
1. 四川农业大学农学院, 四川 成都 611130;
2. 中国科学院上海生命科学研究院植物生理生态研究所, 上海 200032;
3. 中国科学院上海辰山植物科学研究中心, 上海 201602
Author(s):
GUO Yangxin123 WEI Zhiming2 SHE Yuehui1 ZHU Mulan23
1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;
3. Chenshan Botanical Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China
关键词:
大豆叶柄再生体系
Keywords:
soybeanpetioleregeneration system
分类号:
S565.1
DOI:
10.7685/jnau.201703035
摘要:
[目的]建立大豆叶柄外植体的离体再生体系,为大豆遗传转化体系的改进和提高提供技术基础。[方法]以大豆品种‘K06-82’无菌苗叶柄为外植体,比较不同种类的植物生长调节剂及其组合对叶柄愈伤组织诱导、不定芽分化、不定芽伸长及生根等过程的影响,建立大豆叶柄离体再生体系。[结果]大豆品种‘K06-82’的叶柄愈伤组织形成的适宜培养基为改良MS+2.0 mg·L-1α-萘乙酸(NAA),愈伤组织形成率为74.21%,愈伤组织密度为0.72 g·cm-3;不定芽分化的适宜培养基为改良MS+2.0 mg·L-16-苄氨基嘌呤(6-BA)+0.2 mg·L-1 NAA,分化率为49.82%,平均不定芽数2.98个;不定芽伸长的适宜培养基为改良MS+1.0 mg·L-1赤霉素(GA3),伸长比率为35.62%,平均芽长为2.01 cm;不定根诱导的最适培养基为1/2改良MS+0.8 mg·L-1吲哚丁酸(IBA),生根率和生根苗的移栽存活率分别达到88.32%和97.80%。[结论]初步建立了大豆叶柄外植体的离体再生体系。
Abstract:
[Objectives]The purpose of this research is to establish the plantlet regeneration system in soybean. [Methods]By using the aseptic seedling petioles of soybean variety ‘K06-82’ as explants,the effects of different plant growth regulators and their combinations on callus induction and adventitious bud differentiation,adventitious bud elongation and rooting process were compared to establish the regeneration system for soybean stalk in vitro. [Results]The results showed that the suitable medium for callus formation from soybean ‘K06-82’ petiole explants was modified MS+2.0 mg·L-1α-naphthylacetic acid(NAA),on which the callus formation rate was 74.21%,and the density of callus was 0.72 g·cm-3. The optimal medium for the adventitious buds differentiation was modified MS+2.0 mg·L-1 6-benzylamino purine(6-BA) +0.2 mg·L-1 NAA,giving a differentiation frequency of 49.82% and an average number of adventitious buds 2.98. The suitable medium for adventitious bud elongation was modified MS+1.0 mg·L-1gibberellin(GA3). The elongation efficiency was about 35.62%,and the average shoot length was 1.01 cm. Half strength modified MS medium supplemented with 0.8 mg·L-1indole butyric acid(IBA) was the suitable medium for inducing adventitious roots,producing a rooting percentage of 88.32% and a seedling transplanting survival rate of 97.80%. [Conclusions]In this study,the in vitro culture and plantlet regeneration system for soybean petiole explants are established,which will provide the theoretical and technical basis for the establishment of a more perfect soybean genetic transformation system.

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备注/Memo

备注/Memo:
收稿日期:2017-03-24。
基金项目:四川省农业科技成果转化资金项目(16NZ0068);国家转基因生物新品种培育重大专项(2016ZX08004003)
作者简介:郭阳鑫,硕士研究生。
通信作者:朱木兰,研究员,主要从事植物分子遗传研究,E-mail:mlzhu@sippe.ac.cn;佘跃辉,教授,主要从事豆类作物的育种工作,E-mail:228288216@qq.com。
更新日期/Last Update: 1900-01-01