[1]李雪莉,虞徳夫,王超,等.植物乳杆菌制剂对断奶仔猪肠道黏膜功能和微生物菌群及短链脂肪酸的影响[J].南京农业大学学报,2018,41(3):504-510.[doi:10.7685/jnau.201706034]
 LI Xueli,YU Defu,WANG Chao,et al.Effects of Lactobacillus plantarum on intestinal mucosa function, microbial community and short chain fatty acids in weaning piglets[J].Journal of Nanjing Agricultural University,2018,41(3):504-510.[doi:10.7685/jnau.201706034]
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植物乳杆菌制剂对断奶仔猪肠道黏膜功能和微生物菌群及短链脂肪酸的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年3期
页码:
504-510
栏目:
出版日期:
2018-05-15

文章信息/Info

Title:
Effects of Lactobacillus plantarum on intestinal mucosa function, microbial community and short chain fatty acids in weaning piglets
作者:
李雪莉 虞徳夫 王超 丁立人 朱伟云 杭苏琴
南京农业大学消化道微生物研究室, 江苏 南京 210095
Author(s):
LI Xueli YU Defu WANG Chao DING Liren ZHU Weiyun HANG Suqin
Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China
关键词:
植物乳杆菌制剂断奶仔猪营养素转运体紧密连接蛋白短链脂肪酸微生物菌群
Keywords:
Lactobacillus plantarumweaned pigletsnutrient transportertight junction proteinshort chain fatty acids(SCFA)microbial community
分类号:
S816.79
DOI:
10.7685/jnau.201706034
摘要:
[目的]本试验旨在研究植物乳杆菌制剂对苏淮断奶仔猪肠道黏膜营养素转运体、紧密连接蛋白相关基因表达量、微生物菌群数量、短链脂肪酸及乳酸含量的影响。[方法]选取胎次和体质量(9.34 kg)相近的28日龄健康苏淮断奶仔猪144头,按饲喂日粮随机分为对照组(基础日粮)、抗生素组(基础日粮+抗生素)、植物乳杆菌制剂组(基础日粮+植物乳杆菌制剂),每组6个重复,每个重复8头仔猪。预饲期3 d,仔猪32日龄开始试验,59日龄试验结束时屠宰采样。采集十二指肠、空肠、回肠组织及黏膜样品分别用于黏膜营养素转运体和紧密连接蛋白相关基因表达量分析;分别收集盲肠和结肠食糜,测定短链脂肪酸和乳酸含量,RT-qPCR分析微生物菌群数量。[结果]与对照组相比,植物乳杆菌制剂组可显著上调十二指肠PepT1、空肠SGLT-1PepT1以及回肠SGLT-1基因的相对表达量(P<0.05),与抗生素组相比,植物乳杆菌对各基因的相对表达量均无显著影响(P>0.05);与对照组和抗生素组相比,植物乳杆菌制剂组可显著上调十二指肠Occludin、空肠和回肠OccludinZO-1基因的相对表达量(P<0.05),而对十二指肠、空肠、回肠其他基因无明显影响(P>0.05);与对照组相比,植物乳杆菌制剂组可显著增加盲肠丁酸、结肠丁酸和乳酸的含量(P<0.05),而对乙酸和丙酸无显著影响(P>0.05);显著减少大肠杆菌数量(P<0.05),显著增加结肠乳酸杆菌和梭菌ⅩⅣa群的数量(P<0.05);相较于抗生素组,植物乳杆菌制剂可显著提高盲肠乳酸、结肠乳酸和丁酸含量以及盲肠和结肠中乳酸杆菌的数量(P<0.05)。[结论]仔猪断奶时饲喂植物乳杆菌制剂可调节肠道黏膜营养素转运体及紧密连接蛋白相关基因表达量,提高短链脂肪酸和乳酸含量,促进肠道有益菌如乳酸杆菌、梭菌ⅩⅣa群的增殖,减少病原菌如大肠杆菌数量,从而促进肠道健康,缓解断奶应激。
Abstract:
[Objectives] The experiment aims to investigate the effects of Lactobacillus plantarum on genes expression of nutrient transporters, tight junction protein in small intestine mucosa, microbial populations, short chain fatty acids and lactic acid of Suhuai weaned piglets. [Methods] A total of 144 Suhuai weaned piglets(9.34 kg)at age of 28 days were randomly divided into 3 groups, including control group(basal diet), antibiotics group(basal diet supplemented with antibiotics)and L.plantarum group(basal diet supplemented with L.plantarum). Each group had 6 replicates and 8 piglets per replicate. The experiment started on day 32 and ended on day 59 after birth. On day 59, one piglet from each replicate was slaughtered for sampling. On the day of slaughter, the tissue and mucosa samples of duodenum, jejunum and ileum, and digesta samples of cecum and colon were collected, respectively. [Results] Compared with the control group, L.plantarum treatment increased mRNA expression of PepT1 in duodenum(P<0.05), SGLT-1, PepT1 in jejunum and SGLT-1 in ileum(P<0.05). Further, L.plantarum increased mRNA relative expression of Occludin in duodenum, and Occludin, ZO-1 in jejunum and ileum in comparison with the control and antibiotics group(P<0.05). Results also showed that L.plantarum presented a higher concentration of buyrate in cecum, buyrate and lactic acid in colon than that in the control group(P<0.05), while no significant effect was observed on the concentration of acetate and propionate. In terms of bacteria population, L.plantarum decreased the population of Escherichia coli in cecum and colon, increased the population of Lactobacillus and Clostridium cluster ⅩⅣa in colon(P<0.05). In contrast with the antibiotics group, L.plantarum elevated lactic acid concentration in cecum, butyrate and lactic acid concentration in colon, Lactobacillus population in cecum and colon(P<0.05). [Conclusions] Our findings suggest that the administration of L.plantarum could maintain the intestine healthy and relive weaning stress of piglets by regulating gene expression of nutrient transporters and tight junction protein in small intestine mucosa, increasing the short chain fatty acids and lactic acid concentration, promoting the proliferation of Lactobacillus and C.cluster ⅩⅣa and decreasing E.coli population in intestine.

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备注/Memo

备注/Memo:
收稿日期:2017-06-21。
基金项目:国家公益性行业(农业)科研专项(201403047);江苏省农业科技自主创新项目[CX(15)1006]
作者简介:李雪莉,硕士研究生。
通信作者:杭苏琴,教授,主要从事动物生殖道微生态与繁殖、动物营养、肠道微生态与动物健康等研究,E-mail:suqinhang69@njau.edu.cn。
更新日期/Last Update: 1900-01-01