[1]李纯,王超龙,陈赛华,等.水稻RZ54/南京11 F2群体抽穗期QTL分析[J].南京农业大学学报,2018,41(5):801-807.[doi:10.7685/jnau.201803003]
 LI Chun,WANG Chaolong,CHEN Saihua,et al.QTL analysis of heading date in rice RZ54/Nanjing11 F2 population[J].Journal of Nanjing Agricultural University,2018,41(5):801-807.[doi:10.7685/jnau.201803003]
点击复制

水稻RZ54/南京11 F2群体抽穗期QTL分析()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年5期
页码:
801-807
栏目:
出版日期:
2018-09-20

文章信息/Info

Title:
QTL analysis of heading date in rice RZ54/Nanjing11 F2 population
作者:
李纯1 王超龙1 陈赛华1 郑天慧1 鲁健1 崔松1 刘宗凯1 陈亮明1 江玲1 周时荣1 万建民12
1. 南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心, 江苏 南京 210095;
2. 中国农业科学院作物科学研究所, 北京 100081
Author(s):
LI Chun1 WANG Chaolong1 CHEN Saihua1 ZHENG Tianhui1 LU Jian1 CUI Song1 LIU Zongkai1 CHEN Liangming1 JIANG Ling1 ZHOU Shirong1 WAN Jianmin12
1. State Key Laboratory of Crop Genetics and Germplasm Enhancement/Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
水稻抽穗期QTL分析
Keywords:
rice(Oryza sativa L.)heading dateQTL analysis
分类号:
S511.032
DOI:
10.7685/jnau.201803003
摘要:
[目的]挖掘新的控制早熟性的QTL,为早熟性的遗传机制研究和分子育种提供新的基因资源。[方法]选择熟期差异明显的2个品种(早熟品种‘RZ54’和相对迟熟品种‘南京11’)进行杂交,构建F2群体,利用分子标记构建遗传连锁图谱,进行抽穗期QTL定位。[结果]利用141个具有多态性的SSR和Indel标记对由184个株系组成的RZ54/南京11的F2群体进行基因型检测,构建了全长为1 741.4 cM、平均图距为12.35 cM、覆盖水稻12条染色体的遗传图谱。在南京自然光温(高温长日照)环境下检测该群体的抽穗期QTL,结果在第6、7、8及11染色体上发现5个与抽穗期有关的QTL位点,分别命名为qHd-6、qHd-7、qHd-8-1、qHd-8-2qHd-11。其中在qHd-8-1效应区段内存在已报道的基因DTH8,在其他效应区段内未发现已报道的抽穗期基因。但已报道的Hd1Ghd7基因分别位于qHd-6qHd-7位点附近,在第6、7染色体检测到的QTL可能来自这2个基因的效应。因此,qHd-8-2qHd-11可能是新的抽穗期QTL位点。[结论]通过遗传图谱构建和QTL位点分析,检测到了新的QTL位点,为下一步精细定位和图位克隆奠定了基础,也为抽穗期的分子标记辅助育种提供了新的基因资源。
Abstract:
[Objectives]This study aims to find new QTL controlling heading date which was detected in RZ54/Nanjing11 F2 population under natural-field condition in Nanjing.[Methods]In this study,we utilized two materials with different heading date, ‘RZ54’ and ‘Nanjing 11’,to generate a segregation F2 population and construct molecular linkage map. On the basis of that QTL controlling heading date was detected and analyzed.[Results]A molecular linkage map,consisting of 141 markers spanning a total of 1 741.4 cM and covering twelve chromosomes,with an average marker interval of 12.35 cM,was constructed in 184 lines of RZ54/Nanjing11 F2 population. Marker order on the chromosomes matched well with that of the published map. QTL analysis was performed under natural high temperature long day condition in Nanjing. We found that there were five significant sites exist at the sixth,seventh,eighth and eleventh chromosome,named qHd-6,qHd-7,qHd-8-1,qHd-8-2 and qHd-11 respectively. Further analysis revealed that a known gene,DTH8,is located at the identified qHd-8-1 site. There were no reported heading date genes located at other identified sites. However,a known gene Hd1 is located downstream of the identified qHd-6 site,and another known gene,Ghd7,is located upstream of the identified qHd-7 site. The QTL detected on the sixth and seventh chromosome may be derived from the effects of these two genes. These results suggest that the qHd-8-2 and qHd-11 sites are the newly discovered heading date QTL.[Conclusions]New QTL loci were identified in this study,which laid the foundation for further fine mapping and map-based cloning. It also provided new gene resources for molecular marker-assisted breeding of heading date.

参考文献/References:

[1] Yano M,Katayose Y,Ashikari M,et al. Hd1,a major photoperiod sensitivity quantitative trait locus in rice,is closely related to the Arabidopsis flowering time gene CONSTANS[J]. Plant Cell,2000,12(12):2473-2483.
[2] Yano M,Harushima Y,Nagamura Y,et al. Identification of quantitative trait loci controlling heading date in rice using a high-density linkage map[J]. Theoretical and Applied Genetics,1997,95(7):1025-1032.
[3] Yamamoto T,Kuboki Y,Lin S Y,et al. Fine mapping of quantitative trait loci Hd-1,Hd-2 and Hd-3,controlling heading date of rice,as single Mendelian factors[J]. Theoretical and Applied Genetics,1998,97(1/2):37-44.
[4] Monna L,Lin H X,Kojima S,et al. Genetic dissection of a genomic region for a quantitative trait locus,Hd3,into two loci,Hd3a and Hd3b,controlling heading date in rice[J]. Theoretical and Applied Genetics,2002,104(5):772-778.
[5] Lin H X,Liang Z W,Sasaki T,et al. Fine mapping and characterization of quantitative trait loci Hd4 and Hd5 controlling heading date in rice[J]. Breeding Science,2003,53(1):51-59.
[6] 郭龙彪,罗利军,邢永忠,等. 汕优63重组自交系群体重要农艺性状遗传分析和利用[J]. 作物学报,2002,28(5):644-649. Guo L B,Luo L J,Xing Y Z,et al. Genetic analysis and utilization of the important agronomic traits on Zhenshan 97×Minghui 63 recombinant inbred lines(RIL)in rice(Oryza sativa L.)[J]. Acta Agronomica Sinica,2002,28(5):644-649(in Chinese with English abstract).
[7] 陈俊宇. 水稻抽穗期和产量性状微效QTL qHd1的精细定位[D]. 北京:中国农业科学院,2016. Chen J Y.Fine mapping of qHd1,a minor QTL having pleiotropic effects for heading date and yield traits in indica rice[D]. Beijing:Chinese Academy of Agricultural Sciences,2016(in Chinese with English abstract).
[8] Matsubara K,Hori K,Ogiso-Tanaka E,et al. Cloning of quantitative trait genes from rice reveals conservation and divergence of photoperiod flowering pathways in Arabidopsis and rice[J]. Frontiers in Plant Science,2014,5:193.
[9] Tsuji H,Taoka K,Shimamoto K. Florigen in rice:complex gene network for florigen transcription,florigen activation complex,and multiple functions[J]. Current Opinion in Plant Biology,2013,16(2):228-235.
[10] Ishikawa R,Aoki M,Kurotani K,et al. Phytochrome B regulates heading date 1(Hd1)-mediated expression of rice florigen Hd3a and critical day length in rice[J]. Molecular Genetics and Genomics,2011,285(6):461-470.
[11] Tamaki S,Tsuji H,Matsumoto A,et al. FT-like proteins induce transposon silencing in the shoot apex during floral induction in rice[J]. Proc Natl Acad Sci USA,2015,112(8):E901-E910.
[12] Naranjo L,Talon M,Domingo C. Diversity of floral regulatory genes of japonica rice cultivated at northern latitudes[J]. BMC Genomics,2014,15:101.
[13] Doi K,Izawa T,Fuse T,et al. Ehd1,a B-type response regulator in rice,confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1l[J]. Genes and Development,2004,18(8):926-936.
[14] Hori K,Ogiso-Tanaka E,Matsubara K,et al. Hd16,a gene for casein kinase I,is involved in the control of rice flowering time by modulating the day-length response[J]. Plant Journal,2013,76(1):36-46.
[15] Weng X,Wang L,Wang J,et al. Grain number,plant height,and heading date7 is a central regulator of growth,development,and stress response[J]. Plant Physiol,2014,164(2):735-747.
[16] Xue W Y,Xing Y Z,Weng X Y,et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]. Nature Genetics,2008,40(6):761-767.
[17] Yan W H,Wang P,Chen H X,et al. A major QTL,Ghd8,plays pleiotropic roles in regulating grain productivity,plant height,and heading date in rice[J]. Molecular Plant,2011,4(2):319-330.
[18] Wei X,Xu J,Guo H,et al. DTH8 suppresses flowering in rice,influencing plant height and yield potential simultaneously[J]. Plant Physiology,2010,153(4):1747-1758.
[19] Wu W X,Zheng X M,Lu G W,et al. Association of functional nucleotide polymorphisms at DTH2 with the northward expansion of rice cultivation in Asia[J]. Proc Natl Acad Sci USA,2013,110(8):2775-2780.
[20] 沈子杰,范德佳,何俊,等. 籼稻品种‘IR13427-45-2-1-2-2-2’抗白背飞虱QTL定位[J]. 南京农业大学学报,2017,40(6):957-962. DOI:10.7685/jnau.201704027. Shen Z J,Fan D J,He J,et al. Mapping of the quantitative trait locus conferring white-backed planthopper resistance in indica cultivar ‘IR13427-45-2-1-2-2-2’[J]. Journal of Nanjing Agricultural University,2017,40(6):957-962(in Chinese with English abstract).
[21] Li H,Ye G,Wang J. A modified algorithm for the improvement of composite interval mapping[J]. Genetics,2007,175(1):361-374.
[22] Darvasi A,Soller M. A simple method to calculate resolving power and confidence interval of QTL map location[J]. Behavior Genetics,1997,27(2):125-132.
[23] 苏成付,赵团结,盖钧镒. 不同统计遗传模型QTL定位方法应用效果的模拟比较[J]. 作物学报,2010,36(7):1100-1107. Sun C F,Zhao T J,Gei J Y.Simulation comparisons of effectiveness among QTL mapping procedures of different statistical genetic models[J]. Acta Agronomica Sinica,2010,36(7):1100-1107(in Chinese with English abstract).
[24] Lee H S,Fischer R L,Goldberg R B,et al. Arabidopsis LEAFY COTYLEDON1 represents a functionally specialized subunit of the CCAAT binding transcription factor[J]. Proc Natl Acad Sci USA,2003,100(4):2152-2156.
[25] 魏鑫. 基于核苷酸多态性的亚洲栽培稻起源进化研究[D]. 武汉:华中农业大学,2013. Wei X.Origin and evolution of Oryza sativa revealed by nucleotide polymorphisms[D]. Wuhan:Huazhong Agricultural University,2013(in Chinese with English abstract).

相似文献/References:

[1]张辰明,徐烨红,赵海娟,等.不同氮形态对水稻苗期氮素吸收和根系生长的影响[J].南京农业大学学报,2011,34(3):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
 ZHANG Chen-ming,XU Ye-hong,ZHAO Hai-juan,et al.Effects of different nitrogen forms on nitrogen uptake and root growth of rice at the seedling stage[J].Journal of Nanjing Agricultural University,2011,34(5):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
[2]郝文雅,沈其荣,冉炜,等.西瓜和水稻根系分泌物中糖和氨基酸对西瓜枯萎病病原菌生长的影响[J].南京农业大学学报,2011,34(3):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
 HAO Wen-ya,SHEN Qi-rong,RAN Wei,et al.The effects of sugars and amino acids in watermelon and rice root exudates on the growth of Fusarium oxysporum f.sp. niveum[J].Journal of Nanjing Agricultural University,2011,34(5):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
[3]徐小飒,刘喜,赵志刚,等.培矮64S/93-11重组自交系分子图谱构建及千粒重QTL检测[J].南京农业大学学报,2011,34(1):8.[doi:10.7685/j.issn.1000-2030.2011.01.002]
 XU Xiao-sa,LIU Xi,ZHAO Zhi-gang,et al.Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight[J].Journal of Nanjing Agricultural University,2011,34(5):8.[doi:10.7685/j.issn.1000-2030.2011.01.002]
[4]魏广彬,徐海港,丁艳峰,等.水稻设计栽培系统的研制与实现[J].南京农业大学学报,2011,34(1):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
 WEI Guang-bin,XU Hai-gang,DING Yan-feng,et al.Development and realization of the rice design cultivation system[J].Journal of Nanjing Agricultural University,2011,34(5):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
[5]李刚华,王惠芝,王绍华,等.穗肥对水稻穗分化期碳氮代谢及颖花数的影响[J].南京农业大学学报,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
 LI Gang-hua,WANG Hui-zhi,WANG Shao-hua,et al.Effect of nitrogen applied at rice panicle initiation stage on carbon and nitrogen metabolism and spikelets per panicle[J].Journal of Nanjing Agricultural University,2010,33(5):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
[6]王碧茜,范晓荣,徐国华,等.不同氮效率水稻品种旗叶的衰老特征[J].南京农业大学学报,2010,33(2):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
 WANG Bi-qian,FAN Xiao-rong,XU Guo-hua,et al.Characteristics of flag leaf senescence among three rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(5):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
[7]赵成国,徐海港,李刚华,等.超高产单季粳稻抽穗期群体构成研究[J].南京农业大学学报,2011,34(2):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
 ZHAO Cheng-guo,XU Hai-gang,LI Gang-hua,et al.Studies on population composition of super-high-yielding single-cropping japonica rice in heading stage[J].Journal of Nanjing Agricultural University,2011,34(5):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
[8]陈志德,仲维功,王军,等.水稻苗期Cd2+胁迫的QTL定位研究[J].南京农业大学学报,2010,33(3):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
 CHEN Zhi-de,ZHONG Wei-gong,WANG Jun,et al.Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2010,33(5):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
[9]叶利庭,樊剑波,徐晔红,等.不同氮效率水稻的生长特性[J].南京农业大学学报,2010,33(3):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
 YE Li-ting,FAN Jian-bo,XU Ye-hong,et al.Characteristics of growth in rice genotypes with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(5):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
[10]晋玉宽,杨世湖,余丽,等.不同启动子驱动下Pib基因的表达及与稻瘟病抗性的关系[J].南京农业大学学报,2010,33(4):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
 JIN Yu-kuan,YANG Shi-hu,YU Li,et al.Expression and resistance analysis of the Pib gene in transgenic rice under different promoters[J].Journal of Nanjing Agricultural University,2010,33(5):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
[11]吴晶晶,魏子尧,杨春艳,等.控制水稻抽穗延迟基因OsSET34的鉴定和图位克隆[J].南京农业大学学报,2015,38(5):704.[doi:10.7685/j.issn.1000-2030.2015.05.002]
 WU Jingjing,WEI Ziyao,YANG Chunyan,et al.Identification and map-based cloning of OsSET34,a gene controling the delay of heading date in rice(Oryza sativa L).[J].Journal of Nanjing Agricultural University,2015,38(5):704.[doi:10.7685/j.issn.1000-2030.2015.05.002]

备注/Memo

备注/Memo:
收稿日期:2018-3-1。
基金项目:国家重点研发计划项目(2017YFD0100305);江苏省科技支撑计划项目(BE2017368);江苏省农业科技自主创新项目[CX(16)1029];安徽省科技重大专项(16030701068)
作者简介:李纯,硕士研究生。
通信作者:周时荣,教授,博导,研究方向为作物遗传育种,E-mail:srzhou@njau.edu.cn。
更新日期/Last Update: 1900-01-01