LIANG Yongfu,WANG Kangcai,XUE Qi,et al.Effects of salicylic acid on physiological and photosynthetic characteristics of Polygonatum cyrtonema Hua under high temperature and strong light stress[J].Journal of Nanjing Agricultural University,2018,41(5):839-847.[doi:10.7685/jnau.201712014]





Effects of salicylic acid on physiological and photosynthetic characteristics of Polygonatum cyrtonema Hua under high temperature and strong light stress
梁永富 王康才 薛启 隋利 易家宁
南京农业大学园艺学院, 江苏 南京 210095
LIANG Yongfu WANG Kangcai XUE Qi SUI Li YI Jianing
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Polygonatum cyrtonema Huahigh temperaturestrong lightstresssalicylic acidphysiological characteristicsphotosynthetic characteristics
[目的]本文旨在分析水杨酸(SA)对高温强光胁迫下多花黄精生理及光合特性的影响,探讨SA缓解多花黄精高温强光胁迫伤害的可行性。[方法]以遮阴处理的盆栽多花黄精为材料,叶面喷施0.5、1.0、1.5和2.0 mmol·L-1 SA后,撤掉遮阳网,然后进行高温强光胁迫处理,测定并分析胁迫处理期间及25℃遮阴恢复24 h后多花黄精叶片活性氧水平、渗透调节物质含量、抗氧化酶活性、光合及叶绿素荧光指标的变化。[结果]叶面喷施SA可促进多花黄精叶片脯氨酸和可溶性蛋白的积累,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性,降低O2·-产生速率和H2O2含量,使丙二醛(MDA)含量和相对电导率显著下降,增强多花黄精抵御高温强光胁迫伤害的能力。同时,SA还能提高Fv/FmφPSⅡqP,降低NPQ,并能促进气孔开放和CO2转运,使多花黄精能够在逆境胁迫下维持较高的光学活性和净光合速率,但过高SA浓度则会加重多花黄精胁迫伤害。[结论]0.5~1.5 mmol·L-1 SA处理均能提高多花黄精抗逆性,尤其以1.0 mmol·L-1 SA的处理效果最佳。
[Objectives]The effects of salicylic acid(SA) on physiological and photosynthetic characteristics of Polygonatum cyrtonema Hua were studied,in order to reveal the feasibility that SA had a potential to alleviate the stress damages.[Methods]The plants were subjected to high temperature and strong light after a foliar application of SA,with concentrations of 0.5,1.0,1.5 and 2.0 mmol·L-1. The levels of reactive oxygen species(ROS),osmotic adjustment substances contents,antioxidant enzyme activities,changes of photosynthetic and chlorophyll fluorescence indexes were determined during the stress and recover(25℃,shading for 24 h) periods.[Results]The results showed that the accumulation of proline and soluble proteins in leaves was enhanced by foliar application of SA. As well,the activities of superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) increased,and O2·- production rate and H2O2 content,malondialdehyde(MDA) content and relative electrolytic leakage decreased. The above results indicated that the ability of P.cyrtonema resisting high temperature and strong light stress was improved by foliar application of SA. Furthermore,optical activity and net photosynthetic rate of P.cyrtonema exposed to environmental stress were kept in high level since the Fv/Fm,φPSⅡ and qP increased,NPQ decreased,stomatal opening and CO2 was enhanced. However,stress damages caused to P.cyrtonema increased when higher levels of SA were applied.[Conclusions]Stress resistance was enhanced at concentrations of 0.5-1.0 mmol·L-1 SA,particularly 1.0 mmol·L-1.


[1] 国家药典委员会.中华人民共和国药典(一部)[M]. 北京:中国医药科技出版社,2015. Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China(Part one)[M]. Beijing:China Medical Science Press,2015(in Chinese).
[2] 骆绪美,郭婉琳,韩文妍.安徽3种黄精植物光合生理生态特性分析[J]. 安徽农业大学学报,2012,39(5):821-824. Luo X M,Guo W L,Han W Y. Analysis of photosynthetic and physiological characteristics of three species of Polygonatum in Anhui Province[J]. Journal of Anhui Agricultural University,2012,39(5):821-824(in Chinese with English abstract).
[3] 华碧春,马丽娜,宋纬文,等. 论畲药黄精竹林套种模式的适宜性及研究开发[J]. 中国民族医药杂志,2013,19(8):41-43. Hua B C,Ma L N,Song W W,et al. Discussion on the suitability and development of intercropping Polygonatum sibiricum,a traditional Chinese medicine,with bamboo[J]. Journal of Medicine and Pharmacy of Chinese Minorities,2013,19(8):41-43(in Chinese).
[4] 毕研文,杨永恒,宫俊华,等. 光照强度对泰山黄精生长特性及产量的影响[J]. 中国农学通报,2008,24(9):315-319. Bi Y W,Yang Y H,Gong J H,et al. The influence of light to Taishan Sealwort’s growth characteristic and yield[J]. Chinese Agricultural Science Bulletin,2008,24(9):315-319(in Chinese with English abstract).
[5] 李迎春,杨清平,陈双林,等. 光照对多花黄精生长、光合和叶绿素荧光参数特征的影响[J]. 植物研究,2014,34(6):776-781. Li Y C,Yang Q P,Chen S L,et al. Effect of light intensity on growth,photosynthetic and fluorescence characteristic of Polygonatum cyrtonema Hua[J]. Bulletin of Botanical Research,2014,34(6):776-781(in Chinese with English abstract).
[6] Latif F,Ullah F,Mehmood S,et al. Effects of salicylic acid on growth and accumulation of phenolics in Zea mays L. under drought stress[J]. Acta Agriculturae Scandinavica,Section B:Soil & Plant Science,2015,66(4):325-332.
[7] Farooq M,Basra S M A,Wahid A,et al. Improving the drought tolerance in rice(Oryza sativa L.)by exogenous application of salicylic acid[J]. Journal of Agronomy & Crop Science,2009,195(4):237-246.
[8] 马乐元,陈年来,韩国君,等. 外源水杨酸对干旱胁迫下小冠花叶片活性氧水平及抗氧化系统的影响[J]. 草业学报,2017,26(10):129-139. Ma L Y,Chen N L,Han G J,et al. Protective effect of salicylic acid on reactive oxygen levels and antioxidant system of Coronilla varia seedlings under drought stress[J]. Acta Prataculturae Sinica,2017,26(10):129-139(in Chinese with English abstract).
[9] Shi Q,Bao Z,Zhu Z,et al. Effects of different treatments of salicylic acid on heat tolerance,chlorophyll fluorescence,and antioxidant enzyme activity in seedlings of Cucumis sativa L.[J]. Plant Growth Regulation,2006,48(2):127-135.
[10] Khan W,Prithiviraj B,Smith D L. Photosynthetic responses in corn and soybean to foliar application of salicylates[J]. Journal of Plant Physiology,2003,160(5):485-492.
[11] 李新国,毕玉平,赵世杰,等. 短时低温胁迫对甜椒叶绿体超微结构和光系统的影响[J]. 中国农业科学,2005,38(6):1226-1231. Li X G,Bi Y P,Zhao S J,et al. Effects of short-term chilling stress on the photosystems and chloroplast ultrastructure in sweet pepper[J]. Scientia Agricultura Sinica,2005,38(6):1226-1231(in Chinese with English abstract).
[12] 肖家欣.植物生理学实验[M]. 合肥:安徽人民出版社,2010. Xiao J X. Experiment of Plant Physiology[M]. Hefei:Anhui Peoples Publishing House,2010(in Chinese).
[13] Hodgson R A J,Raison J K. Superoxide production by thylakoids during chilling and its implication in the susceptibility of plants to chilling-induced photoinhibition[J]. Planta,1991,183(2):222-228.
[14] Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions[J]. Plant Physiology,2006,141(2):391-396.
[15] Xu S,Li J,Zhang X,et al. Effects of heat acclimation pretreatment on changes of membrane lipid peroxidation,antioxidant metabolites,and ultrastructure of chloroplasts in two cool-season turfgrass species under heat stress[J]. Environmental and Experimental Botany,2006,56(3):274-285.
[16] de Azevedo Neto A D,Prisco J T,Enéas-Filho J,et al. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes[J]. Environmental and Experimental Botany,2006,56(1):87-94.
[17] Chen Z,Ricigliano J W,Klessig D F. Purification and characterization of a soluble salicylic acid-binding protein from tobacco[J]. Proc Natl Acad Sci USA,1993,90(20):9533-9537.
[18] Horváth E,Janda T,Szalai G,et al. In vitro salicylic acid inhibition of catalase activity in maize:differences between the isozymes and a possible role in the induction of chilling tolerance[J]. Plant Science,2002,163(6):1129-1135.
[19] Horváth E,Szalai G,Janda T. Induction of abiotic stress tolerance by salicylic acid signaling[J]. Journal of Plant Growth Regulation,2007,26(3):290-300.
[20] Rao M V,Lee H I,Davis K R. Ozone-induced ethylene production is dependent on salicylic acid,and both salicylic acid and ethylene act in concert to regulate ozone-induced cell death[J]. Plant Journal,2002,32(4):447-456.
[21] 徐娜婷,刘玉民,刘亚敏,等. 引种花卉紫串扦插苗对高温胁迫的生理响应[J]. 草业学报,2012,21(5):257-263. Xu N T,Liu Y M,Liu Y M,et al. Research of high tempreture stress on the morphological and physiological changes in Scaevola albida ‘Mauve Clusters’ cutting seedlings[J]. Acta Prataculturae Sinica,2012,21(5):257-263(in Chinese with English abstract).
[22] 王日明,熊兴耀.高温胁迫对黑麦草生长及生理代谢的影响[J]. 草业学报,2016,25(8):81-90. Wang R M,Xiong X Y. Effect of temperature stress on growth and metabolism in perennial ryegrass[J]. Acta Prataculturae Sinica,2016,25(8):81-90(in Chinese with English abstract).
[23] Khan M I,Iqbal N,Masood A,et al. Salicylic acid alleviates adverse effects of heat stress on photosynthesis through changes in proline production and ethylene formation[J]. Plant Signaling and Behavior,2013,8(11):e26374.
[24] Campos M K F D,Carvalho K D,Souza F S D,et al. Drought tolerance and antioxidant enzymatic activity in transgenic ‘Swingle’ citrumelo plants over-accumulating proline[J]. Environmental and Experimental Botany,2011,72(2):242-250.
[25] Karim A,Fukamachi H,Hidaka T. Photosynthetic performance of Vigna radiate L. leaves developed at different temperature and irradiance levels[J]. Plant Science,2003,164(4):451-458.
[26] Yamamoto Y,Aminaka R,Yoshioka M,et al. Quality control of photosystem Ⅱ:impact of light and heat stresses[J]. Photosynthesis Research,2008,98(1/2/3):589-608.
[27] Nishiyama Y. Photoinhibition of photosystem Ⅱ under environmental stress[J]. Biochimica et Biophysica Acta,2007,1767(6):414-421.
[28] 马培芳,李利红,杨亚军,等. 水杨酸对高温强光胁迫下小麦叶绿体D1蛋白磷酸化及光系统Ⅱ功能的影响[J]. 应用生态学报,2008,19(12):2632-2636. Ma P F,Li L H,Yang Y J,et al. Effects of salicylic acid on D1 protein phosphorylation and PSⅡ performance in wheat leaf chloroplasts under high temperature and high light stress[J]. Chinese Journal of Applied Ecology,2008,19(12):2632-2636(in Chinese with English abstract).
[29] 郑静静,杨丽,苏小雨,等. 水杨酸对高温强光下小麦叶绿体蛋白酶Deg5和PS Ⅱ功能的调节作用[J]. 生态学报,2014,34(24):7350-7355. Zheng J J,Yang L,Su X Y,et al. Regulatory effects of salicylic acid on protease Deg5 and PSⅡ function of wheat chloroplasts under heat and high light stress[J]. Acta Ecologica Sinica,2014,34(24):7350-7355(in Chinese with English abstract).
[30] Singh B,Usha K. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress[J]. Plant Growth Regulation,2003,39(2):137-141.


 GUO Liang,DU Juan,DI He-shuang,et al.Effects of unitary or successive heat treatment on mammary epithelial cells proliferation and apoptosis[J].Journal of Nanjing Agricultural University,2007,30(5):100.[doi:10.7685/j.issn.1000-2030.2007.03.019]
 LI Yan,LIU Ming,WANG Gaopeng,et al.Effect of high temperature at vegetative stage on chilling tolerance of rice during reproductive period[J].Journal of Nanjing Agricultural University,2016,39(5):10.[doi:10.7685/jnau.201504048]


更新日期/Last Update: 1900-01-01