[1]房加鹏,张琳,李茹,等.稗HYYJ-1种群对五氟磺草胺的抗药性及其与细胞色素P450代谢的关系[J].南京农业大学学报,2018,41(5):854-859.[doi:10.7685/jnau.201711036]
 FANG Jiapeng,ZHANG Lin,LI Ru,et al.P450-mediated metabolism,related to the penoxsulam resistance in barnyard grass(Echinochloa crusgalli)[J].Journal of Nanjing Agricultural University,2018,41(5):854-859.[doi:10.7685/jnau.201711036]
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稗HYYJ-1种群对五氟磺草胺的抗药性及其与细胞色素P450代谢的关系()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
41卷
期数:
2018年5期
页码:
854-859
栏目:
出版日期:
2018-09-20

文章信息/Info

Title:
P450-mediated metabolism,related to the penoxsulam resistance in barnyard grass(Echinochloa crusgalli)
作者:
房加鹏1 张琳2 李茹3 陈国奇1 董立尧1
1. 南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室, 江苏 南京 210095;
2. 上海青浦区农业技术推广服务中心, 上海 201700;
3. 徐淮地区淮阴农业科学研究院, 江苏 淮安 223001
Author(s):
FANG Jiapeng1 ZHANG Lin2 LI Ru3 CHEN Guoqi1 DONG Liyao1
1. College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China;
2. Shanghai Qingpu District Agricultural Technology Extension Service Center, Shanghai 201700, China;
3. Xuhuai District Huaiyin Academy of Agricultural Sciences, Huai’an 223001, China
关键词:
五氟磺草胺乙酰乳酸合成酶P450代谢
Keywords:
Echinochloa crusgallipenoxsulamacetolactate synthase(ALS)P450-mediated metabolism
分类号:
S451
DOI:
10.7685/jnau.201711036
摘要:
[目的]本文旨在明确稗[Echinochloa crusgalli(L.)P.Beauv]对五氟磺草胺产生抗药性的机制。[方法]采用整株生物测定的方法研究3种细胞色素P450抑制剂(PBO、ABT和马拉硫磷)对抗五氟磺草胺稗HYYJ-1种群敏感性的影响;以敏感种群JLGY-3为对照,应用分子生物学等方法进行了抗性种群乙酰乳酸合成酶(acetolactate synthase,ALS)活性、ALS基因序列及ALS基因表达量的研究。[结果]发现P450抑制剂PBO、ABT和马拉硫磷均能显著提高抗性种群对五氟磺草胺的敏感性,鲜质量抑制中剂量(ED50,以药剂有效成分计),从27.16 g·hm-2下降为3.44、6.06和3.23 g·hm-2;通过ALS基因序列比对,未发现HYYJ-1种群ALS基因发生氨基酸替换;抗性和敏感种群ALS离体酶活性及基因表达量差异不明显。[结论]抗性种群HYYJ-1对五氟磺草胺产生抗药性不是ALS靶标酶变化所致,而与细胞色素P450代谢作用加强有关。
Abstract:
[Objectives]The present study aimed to explore the mechanisms of penoxsulam resistance in barnyard grass.[Methods]By whole-plant bioassay,the sensitivity of HYYJ-1 population to penoxsulam with/without P450 inhibitors(PBO,ABT and Malathion) was determined. Molecular biology methods were used to analyze the acetolactate synthase(ALS) gene sequence,the activity of ALS in vitro and the expression of ALS gene between the two populations.[Results]In the involvement of P450 inhibitors PBO,ABT and Malathion,whole-plant bioassays indicated that the ED50 of HYYJ-1 population reduced to 3.44,6.06 and 3.23 g·hm-2 from 27.16 g·hm-2. As for target site mechanisms,no known mutant that confers resistance to ALS-inhibitors was detected in resistant population;also,the ALS activity and gene expression level between the two populations were close to each other.[Conclusions]Target-site based mechanisms were not the reason for the resistance of HYYJ-1 population to penoxsulam,but the enhancement of metabolism herbicides mediated by cytochrome P450 seems to be the cause.

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备注/Memo

备注/Memo:
收稿日期:2017-11-26。
基金项目:国家自然科学基金项目(31572021);国家重点研发计划项目(2018YFD0200300)
作者简介:房加鹏,硕士研究生。
通信作者:董立尧,教授,博导,研究方向为除草剂毒理及抗药性,E-mail:dly@njau.edu.cn。
更新日期/Last Update: 1900-01-01