[1]陶蓉,潘根,江玲,等.籼稻品种‘BP360e’抗褐飞虱遗传分析及基因定位[J].南京农业大学学报,2019,42(1):14-20.[doi:10.7685/jnau.201803053]
 TAO Rong,PAN Gen,JIANG Ling,et al.Genetic analysis and mapping of the gene conferring brown planthopper resistance in indica rice cultivar ‘BP360e’[J].Journal of Nanjing Agricultural University,2019,42(1):14-20.[doi:10.7685/jnau.201803053]
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籼稻品种‘BP360e’抗褐飞虱遗传分析及基因定位()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年1期
页码:
14-20
栏目:
植物科学
出版日期:
2019-01-09

文章信息/Info

Title:
Genetic analysis and mapping of the gene conferring brown planthopper resistance in indica rice cultivar ‘BP360e’
作者:
陶蓉1 潘根1 江玲1 程遐年1 刘裕强1 万建民12
1. 南京农业大学作物遗传与种质创新国家重点实验室, 江苏 南京 210095;
2. 中国农业科学院作物科学研究所, 北京 100081
Author(s):
TAO Rong1 PAN Gen1 JIANG Ling1 CHENG Xianian1 LIU Yuqiang1 WAN Jianmin12
1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
水稻褐飞虱抗性数量性状基因座
Keywords:
Oryza sativa L.brown planthopperresistancequantitative trait loci (QTL)
分类号:
S511.032
DOI:
10.7685/jnau.201803053
摘要:
[目的]本研究旨在发掘水稻抗褐飞虱基因,为抗褐飞虱品种培育提供有用的基因资源。[方法]利用印尼抗褐飞虱籼稻品种‘BP360e’与感虫品种‘02428’分别构建F2:3和BC2F2:3分离群体,采用苗期集团法进行抗褐飞虱表型鉴定。利用151对均匀分布在水稻12条染色体的多态性标记,选取F2:3群体中的极端个体进行初连锁分析,构建具有连锁迹象的染色体连锁图谱进行QTL(quantitative trait loci)检测。再利用BC2F2:3群体验证F2:3群体检测到QTL的可靠性。[结果]在第4染色体标记RM16382与INDEL4-5之间,约15.3 cM的区间内检测到1个抗褐飞虱主效QTL,LOD值为14.4,贡献率为56%,将其命名为QBph4。用BC2F2:3群体重复检测到该QTL。该区间包含已克隆的抗褐飞虱基因Bph3。序列分析表明,QBph4可能为Bph3的一种新的等位基因。[结论]‘BP360e’高抗褐飞虱,其抗性受1对主效QTL控制,该抗性基因的发掘为水稻抗褐飞虱分子机制阐明及新品种培育奠定了基础。
Abstract:
[Objectives]The aim of present study is to explore novel BPH resistance gene for breeding BPH resistance rice cultivars. [Methods]An F2:3 and a BC2F2:3 populations were constructed by crossing resistant indica cultivar ‘BP360e’ with susceptible japonica cultivar ‘02428’,respectively,and then evaluated for BPH resistance by standard seed box screening test. Further,151 polymorphic SSR markers between the parents uniformly distributed on 12 chromosomes of rice,extreme resistant and susceptible lines selected from F2:3 population were used primarily for linkage analysis,and the molecular linkage map of candidate chromosome was applied to detect the QTL conferring BPH resistance. Then,the QTL was verified by BC2F2:3 population. [Results]A major QTL on chromosome 4 was detected in the 15.3 cM interval of RM16382 and INDEL4-5,with LOD score of 14.4,explained the phenotypic variance of 56%,and subsequently designated as QBph4. Sequencing analysis showed that QBph4 probably was a novel allele for Bph3,a previously cloned BPH resistance gene. [Conclusions]The indica cultivar ‘BP360e’ had a highly resistance against BPH,which was contributed by a major QTL. The discovery of this resistance gene has laid a foundation for elucidating the underling mechanism of BPH resistance and breeding resistance rice cultivars.

参考文献/References:

[1] 程遐年,吴进才,马飞. 褐飞虱研究与防治[M]. 北京:中国农业出版社,2003:27-58. Cheng X N,Wu J C,Ma F. Research and prevention of brown planthopper[M]. Beijing:China Agriculture Press,2003:27-58(in Chinese).
[2] 王彦华,王强,沈晋良,等. 褐飞虱抗药性研究现状[J]. 昆虫知识,2009,46(4):518-524. Wang Y H,Wang Q,Shen J L,et al. Current status of insecticide resistance in the brown planthopper,Nilaparvata lugens[J]. Chinese Bulletin of Entomology,2009,46(4):518-524(in Chinese with English abstract).
[3] 王鹏,甯佐苹,张帅,等. 我国主要稻区褐飞虱对常用杀虫剂的抗性监测[J]. 中国水稻科学,2013(2):191-197. Wang P,Ning Z P,Zhang S,et al. Resistance monitoring to conventional insecticides in brown planthopper,Nilaparvata lugens in main rice growing regions in China[J]. Chinese Journal of Rice Science,2013(2):191-197(in Chinese with English abstract).
[4] Liu Y,Wu H,Chen H,et al. A gene cluster encoding lectin receptor kinases confers broad-spectrum and durable insect resistance in rice[J]. Nature Biotechnology,2015,33(3):301-305.
[5] Shang G X,Zhang J,Liu B,et al. A mucin-like protein of planthopper is required for feeding and induces immunity response in plants[J]. Plant Physiology,2018,176(1):552-555.
[6] Zhao Y,Huang J,Wang Z,et al. Allelic diversity in an NLR gene BPH9 enables rice to combat planthopper variation[J]. Proc Natl Acad Sci USA,2016,113(45):12850-12855.
[7] Ren J,Gao F,Wu X,et al. Bph32,a novel gene encoding an unknown SCR domain-containing protein,confers resistance against the brown planthopper in rice[J]. Scientific Reports,2016,6(1):37645.
[8] Guo J,Xu C,Wu D,et al. Bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice[J]. Nature Genetics,2018,50(2):297-306.
[9] Cha Y S,Ji H,Yun D W,et al. Fine mapping of the rice bph1 gene,which confers resistance to the brown planthopper,and development of STS markers for marker-assisted selection[J]. Molecular Cell,2008,26(2):146-151.
[10] Du B,Zhang W,Liu B,et al. Identification and characterization of Bph14,a gene conferring resistance to brown planthopper in rice[J]. Proc Natl Acad Sci USA,2009,106(52):22163-22168.
[11] Tamura Y,Hattori M,Yoshioka H,et al. Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52[J]. Scientific Reports,2015,4(1):5872.
[12] Wang Y,Cao L,Zhang Y,et al. Map-based cloning and characterization of BPH29,a B3 domain-containing recessive gene conferring brown planthopper resistance in rice[J]. Journal of Experimental Botany,2015,66(19):6035-6045.
[13] Ji H,Kim S R,Kim Y H,et al. Map-based cloning and characterization of the BPH18 gene from wild rice conferring resistance to brown planthopper(BPH) insect pest[J]. Scientific Reports,2016,6(1):36688.
[14] 肖聪. 水稻褐飞虱抗性基因的遗传定位及近等基因系的构建和抗性评价[D]. 武汉:华中农业大学,2017:21-36. Xiao C. Mapping brown planthopper resistance genes and development and evaluation of near isogenic lines for brown planthopper resistance in rice[D]. Wuhan:Huazhong Agricultural University,2017:21-36(in Chinese with English abstract).
[15] 周亦红,韩召军. 褐飞虱生物型研究进展:致害性变异的遗传机制[J]. 昆虫知识,2003,40(3):199-203. Zhou Y H,Han Z J. The brown planthopper biotypes:on the genetic mechanism of virulence[J]. Entomological Knowledge,2003,40(3):199-203(in Chinese with English abstract).
[16] 任西明,向聪,雷东阳,等. 水稻抗褐飞虱育种研究进展与展望[J]. 作物研究,2017,31(4):453-457. Ren X M,Xiang C,Lei D Y,et al. Present status and prospect of resistance breeding of brown planthopper in rice[J]. Crop Research,2017,31(4):453-457(in Chinese with English abstract).
[17] Kumar K,Sarao P S,Bhatia D,et al. High resolution genetic mapping of a novel brown planthopper resistance locus,Bph34 in Oryza sativa L.×Oryza nivara(Sharma & Shastry) derived interspecifc F2 population[J]. Theoretical and Applied Genetics,2018,131(5):1163-1171.
[18] Huang D,Qiu Y,Zhang Y,et al. Fine mapping and characterization of BPH27,a brown planthopper resistance gene from wild rice(Oryza rufipogon Griff.)[J]. Theoretical and Applied Genetics,2013,126(1):219-229.
[19] Qiu Y,Guo J,Jing S,et al. High-resolution mapping of the brown planthopper resistance gene Bph6 in rice and characterizing its resistance in the 9311 and Nipponbare near isogenic backgrounds[J]. Theoretical and Applied Genetics,2010,121(8):1601-1611.
[20] IRRI. Standard Evaluation System For Rice[M]. Manila:International Rice Research Institute,Philippines,1988:1-56.
[21] 刘仁虎,孟金陵. MapDraw,在Excel中绘制遗传连锁图的宏[J]. 遗传,2003,25(3):317-321. Liu R H,Meng J L. MapDraw:a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data[J]. Hereditas,2003,25(3):317-321(in Chinese with English abstract).
[22] 高希武,彭丽年,梁帝允. 对2005年水稻褐飞虱大发生的思考[J]. 植物保护,2006,32(2):23-24. Gao W X,Peng L N,Liang D Y. Factors causing the outbreak of brown planthopper(BHP),Nilapavata lugens St?l in China in 2005[J]. Plant Protection,2006,32(2):23-24(in Chinese with English abstract).
[23] 陈建明. 水稻品种对褐飞虱为害的耐性及其生理机制研究[D]. 杭州:浙江大学,2004:19-41. Chen J M. Tolerance of rice varieties to the brown planthopper,Nilaparvata lugens St?l and its physiological[D]. Hangzhou:Zhejiang University,2004:19-41(in Chinese with English abstract).
[24] 沈子杰,范德佳,何俊,等. 籼稻品种‘IR13427-45-2-1-2-2-2’抗白背飞虱QTL定位[J]. 南京农业大学学报,2017,40(6):957-962. DOI:10.7685/jnau.201704027. Shen Z J,Fan D J,He J,et al. Mapping of the quantitative trait locus conferring white-backed planthopper resistance in indica cultivar ‘IR13427-45-2-1-2-2-2’[J]. Journal of Nanjing Agricultural University,2017,40(6):957-962(in Chinese with English abstrace).
[25] 吴寒. 水稻抗褐飞虱基因Bph3的图位克隆和功能研究[D]. 南京:南京农业大学,2013:8-37. Wu H. Map-based cloning and functional analysis of brown planthopper resistance gene Bph3 in rice[D]. Nanjing:Nanjing Agricultural University,2013:8-37(in Chinese with English abstract).
[26] Lehti-Shiu M D,Shiu S H. Diversity,classification and function of the plant protein kinase superfamily[J]. Philosophical Transactions of the Royal Society B,2012,367(1602):2619-2639.
[27] Vaid N,Pandey P K,Tuteja N. Genome-wide analysis of lectin receptor-like kinase family from Arabidopsis and rice[J]. Plant Molecular Biology,2012,80(4/5):365-388.
[28] Choi J,Tanaka K,Cao Y R,et al. Identification of a plant receptor for extracellular ATP[J]. Science,2014,343(6168):290-294.

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备注/Memo

备注/Memo:
收稿日期:2018-03-27。
基金项目:国家重点研发计划项目(2016YFD0100603-9);国家自然科学基金项目(31522039,31471470);江苏省自然科学基金项目(BK20150026)
作者简介:陶蓉,硕士研究生。
通信作者:刘裕强,教授,博导,研究方向为水稻遗传育种,E-mail:yql@njau.edu.cn。
更新日期/Last Update: 1900-01-01