[1]王海彦,成城,安立昆,等.玉米内源茉莉酸调节干旱胁迫下的光合作用和抗旱生理反应[J].南京农业大学学报,2019,42(1):30-38.[doi:10.7685/jnau.201803015]
 WANG Haiyan,CHENG Cheng,AN Likun,et al.Endogenous JA regulates leaf photosynthesis and drought-tolerance response under drought condition in maize[J].Journal of Nanjing Agricultural University,2019,42(1):30-38.[doi:10.7685/jnau.201803015]
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玉米内源茉莉酸调节干旱胁迫下的光合作用和抗旱生理反应()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年1期
页码:
30-38
栏目:
植物科学
出版日期:
2019-01-09

文章信息/Info

Title:
Endogenous JA regulates leaf photosynthesis and drought-tolerance response under drought condition in maize
作者:
王海彦 成城 安立昆 秦佳 严远鑫
南京农业大学作物遗传与种质创新国家重点实验室, 江苏 南京 210095
Author(s):
WANG Haiyan CHENG Cheng AN Likun QIN Jia YAN Yuanxin
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
关键词:
玉米干旱茉莉酸光合特性脱落酸
Keywords:
maizedroughtjasmonic acid (JA)photosynthetic characteristicsabscisic acid (ABA)
分类号:
S513
DOI:
10.7685/jnau.201803015
摘要:
[目的]本文旨在探究玉米内源茉莉酸(JA)在抗旱反应中的作用及对内源脱落酸(ABA)合成的影响。[方法]以玉米自交系B73和茉莉酸合成缺少突变体opr7opr8opr)为试验材料,进行土壤干旱处理和快速干旱处理。土壤干旱处理:生长至V7时期植株断水,13 d后复水为干旱处理;以保持土壤湿润为对照。处理后1、4、7、10、13、16、19 d取样。研究干旱胁迫对B73及opr苗期叶片净光合速率(Pn)、气孔导度(Gs)、胞间CO2浓度(Ci)、蒸腾速率(Tr),总超氧化物歧化酶(T-SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,丙二醛(MDA)、脯氨酸、可溶性蛋白含量的影响。快速干旱处理:切取V3时期幼苗地上部分,插入空试管为干旱处理;插入装满无菌水的试管为对照。各处理均置于28℃、相对湿度60%、光照强度为10 000 lx的生长箱中,分别于处理后0、1、3、6、9、12 h取样,采用RT-qPCR方法检测ABA合成通路关键基因ZmZEP1、ZmNCED1ZmAO1的表达情况。[结果]土壤干旱处理:对照中,opr的气孔密度小于B73,Ci值略低;干旱处理后,opr与B73的PnGsTr值均下降,opr更敏感但下降速率慢。干旱7 d后,opr的净光合能力高于B73;T-SOD、POD和CAT活性呈现先上升后下降趋势,opr的酶活性下降时间晚于B73;MDA、可溶性蛋白和游离脯氨酸含量呈上升趋势,但opr的变化幅度小。植株快速干旱处理:opr和B73的ZmNCED1、ZmZEP1ZmAO1表达量在对照中无显著差异,干旱后均有上升,但opr中,ZmNCED1、ZmZEP1ZmAO1表达量低于B73。[结论]opr在土壤干旱条件下能够维持更高的叶片含水量、光合作用效率和抗氧化酶活性,细胞膜损伤较低,表明玉米内源JA在植物的抗旱反应中起重要作用,内源JA的缺少能够减少水分损失,提高在干旱条件下的生存能力。快速干旱条件下,内源JA的缺少降低了ABA合成途径中的3个关键酶活性,说明抗旱反应中ABA的合成被延缓,引起了ABA调控的植物抗旱反应延迟。
Abstract:
[Objectives]This paper is aimed at revealing the role of endogenous jasmonic acid(JA) in drought-tolerance response and its influence to endogenous abscisic acid(ABA) biosynthesis. [Methods]B73 and JA-deficient line opr7opr8(opr) were used to do soil drought and fast drought treatment. Soil drought treatment:plants were stopped watering for 13 days then re-watered as the drought treatment,and the plants watered routinely were used as the control. The leaves of the plants were sampled at 1,4,7,10,13,16 and 19 days of drought treatment period. Photosynthetic parameters such as net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular carbon dioxide concentration(Ci),transpiration rate(Tr) were measured during the experiment time. The activity of antioxidant enzyme includes total-superoxide dismutase(T-SOD),peroxidase(POD),catalase(CAT),malondialdehyde(MDA) content and osmotic adjustment substances(proline and soluble protein) in leaves of the plants were detected. Fast drought treatment:the seedlings of B73 and opr were cutted off at V3 stage,and placed straightly in tubes with(control) or without water(dehydrate treatment). The detached seedlings were placed in a growth chamber where the condition maintained:temperature at 28℃,relative humidity at 60% and illumination at 10 000 lx. The leaves of the plants were sampled at 0,1,3,6,9 h after cut-off. The expression levels of three ABA biosynthesis genes,ZmZEP1,ZmNCED1 and ZmAO1 were detected by quantitative PCR. [Results]Soil drought treatment:opr had lower stomatal density and Ci than B73 under normal condition. Under drought treatment,Pn,Gs and Tr went down both in the opr and B73 leaves,but opr slower than B73,and the Pn of opr was higher than B73 at 7 day. The trend of T-SOD,POD and CAT activities rose up first and went down during drought tolerance. MDA,proline and soluble protein contents went up both in opr and B73,but had a minor variation in opr. Fast drought treatment:there were no significantly difference in the relative expression of gene ZmNCED1,ZmZEP1 and ZmAO1 in control plants,but up-regulated under drought stress. Expression level of gene ZmNCED1,ZmAO1 and ZmZEP1 in opr was lower than B73. [Conclusions]opr mutant has higher water content,photosynthetic efficiency,antioxidant enzyme activity but lower cellular membrane damage under drought treatment. It is indicated that maize endogenous JA plays an important role in drought response. The deficiency of endogenous JA in the mutant delays the drought response of the mutant plants. Under fast drought treatment,the absence of endogenous JA has delayed the activity of 3 key enzymes in the synthesis pathway of ABA,it indicates that the synthesis of ABA in drought response was delayed.

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备注/Memo

备注/Memo:
收稿日期:2018-03-08。
基金项目:国家自然科学基金项目(31571580);南京农业大学人才引进项目(KYRC201404);中央高校基本科研业务费专项资金(KYTZ201402);江苏省现代作物生产协同创新中心项目(No.10)
作者简介:王海彦,硕士研究生。
通信作者:严远鑫,教授,博导,研究方向为玉米遗传育种,E-mail:yuanxin.yan@njau.edu.cn。
更新日期/Last Update: 1900-01-01