[1]段银平,薛瑛,周爽,等.脂肪因子Chemerin对牛卵巢颗粒细胞凋亡与自噬的影响[J].南京农业大学学报,2019,42(1):145-151.[doi:10.7685/jnau.201801017]
 DUAN Yinping,XUE Ying,ZHOU Shuang,et al.Effect of Chemerin on apoptosis and autophagy in bovine granulosa cells[J].Journal of Nanjing Agricultural University,2019,42(1):145-151.[doi:10.7685/jnau.201801017]
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脂肪因子Chemerin对牛卵巢颗粒细胞凋亡与自噬的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年1期
页码:
145-151
栏目:
动物科学
出版日期:
2019-01-09

文章信息/Info

Title:
Effect of Chemerin on apoptosis and autophagy in bovine granulosa cells
作者:
段银平1 薛瑛1 周爽1 张敏1 张宾2 李惠侠1
1. 南京农业大学动物科技学院, 江苏 南京 210095;
2. 新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室, 新疆 石河子 832000
Author(s):
DUAN Yinping1 XUE Ying1 ZHOU Shuang1 ZHANG Min1 ZHANG Bin2 LI Huixia1
1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
2. State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000,
关键词:
Chemerin自噬凋亡卵巢颗粒细胞
Keywords:
Chemerinautophagyapoptosisgranulosa cellsbovine
分类号:
Q952.5
DOI:
10.7685/jnau.201801017
摘要:
[目的]本试验旨在探讨脂肪因子Chemerin在牛卵巢颗粒细胞凋亡与自噬中的作用。[方法]体外培养牛卵巢颗粒细胞并用重组Chemerin蛋白(0.2 μg·mL-1)处理24 h,采用流式细胞术和CCK-8检测细胞凋亡,通过免疫荧光检测细胞中活性氧(ROS)含量变化,测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量;RT-qPCR分析凋亡与自噬相关基因Bax、Bcl-2、Caspase-3、P62Atg7 mRNA表达,Western blot技术检测凋亡与自噬相关蛋白表达情况。[结果]与对照组相比,Chemerin处理卵巢颗粒细胞后,细胞中ROS和MDA含量显著上升(P<0.05),SOD和GSH-px活性极显著降低(P<0.01)。凋亡相关基因BaxCaspase-3 mRNA及蛋白表达水平显著升高,P62Atg7 mRNA等的表达也显著上调(P<0.05)。[结论]Chemerin可通过调控相关基因的表达诱导牛卵巢颗粒细胞凋亡并伴随自噬的发生。
Abstract:
[Objectives]This experiment aims to investigate the effect of Chemerin on apoptosis and autophagy in bovine granulosa cells. [Methods]In this study,granulosa cells were cultured and treated by Chemerin(0.2 μg·mL-1) for 24 h in vitro,apoptosis was detected by flow cytometry and CCK-8,immunofluorescence assay was used to measure intracellular ROS accumulation. SOD,MDA and GSH-px activities in bovine granulosa cells were measured. mRNA expressions of apoptosis and autophagy related genes Bax,Bcl-2,Caspase-3,P62 and Atg7 were checked by quantitative real-time PCR(RT-qPCR). Meanwhile,the related protein expressions of apoptosis and autophagy were detected by Western blot. [Results]Compared with control group,intracellular ROS and MDA content increased(P<0.05),and the activity of glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD)(P<0.01) significantly decreased in Chemerin-treatment cells. In addition,compared with the control group,mRNA and protein expressions of Bax and Caspase-3 significantly increased,and the expression of autophagy related genes were up-regulated significantly by Chemerin-treatment cells(P<0.05). [Conclusions]These results indicated that Chemerin could promote apoptosis and autophagy by regulating relative gene expression.

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备注/Memo

备注/Memo:
收稿日期:2018-01-15。
基金项目:江苏省农业科技自主创新资金项目[CX(17)1005];江苏省自然科学基金项目(BK20171381);江苏省高等学校大学生创新创业训练计划项目(201710307012X)
作者简介:段银平,硕士研究生。
通信作者:李惠侠,教授,主要从事动物主要性状形成的生理学相关研究,E-mail:lihuixia@njau.edu.cn。
更新日期/Last Update: 1900-01-01