[1]安世钰,张国敏,尤佩华,等.PPARGC1A/NRF1/TFAM通路核心基因在湖羊睾丸发育过程中的表达变化[J].南京农业大学学报,2019,42(2):345-351.[doi:10.7685/jnau.201806041]
　AN Shiyu,ZHANG Guomin,YOU Peihua,et al.Research on the change of PPARGC1A/NRF1/TFAM pathway genes expression in the development of Hu sheep testis[J].Journal of Nanjing Agricultural University,2019,42(2):345-351.[doi:10.7685/jnau.201806041]
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PPARGC1A/NRF1/TFAM通路核心基因在湖羊睾丸发育过程中的表达变化()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
42卷
期数:
2019年2期
页码:
345-351
栏目:
动物科学
出版日期:
2019-03-19

文章信息/Info

Title:
Research on the change of PPARGC1A/NRF1/TFAM pathway genes expression in the development of Hu sheep testis
作者:
安世钰1;  张国敏1;  尤佩华2;  孟繁星3;  刘建玲1;  张婷婷1;  杨花1;  樊懿萱1;  王锋1
1. 南京农业大学江苏省家畜胚胎工程实验室, 江苏 南京 210095;
2. 江苏波杜农牧股份有限公司, 江苏 南京 211803;
3. 南京农业大学动物科学类国家级实验教学示范中心, 江苏 南京 210095
Author(s):
AN Shiyu1;  ZHANG Guomin1;  YOU Peihua2;  MENG Fanxing3;  LIU Jianling1;  ZHANG Tingting1;  YANG Hua1;  FAN Yixuan1;  WANG Feng1
1. Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, China;
2. Jiangsu AgriPortal Company Limited, Nanjing 211803, China;
3. National Experimental Teaching Demonstration Center for Animal Science, Nanjing Agricultural University, Nanjing 210095, China
关键词:
湖羊; 睾丸; 性成熟前后; PPARGC1A/RNF1/TFAM通路
Keywords:
Hu sheep; testis; prepubertal and postpubertal; PPARGC1A/RNF1/TFAM pathway
分类号:
Q812
DOI:
10.7685/jnau.201806041
摘要:
[目的] 本试验旨在探究PPARGC1A/NRF1/TFAM通路核心基因在湖羊性成熟前后的表达变化。[方法] 选取体况良好和系谱清楚的雄性湖羊18只（3和9月龄，各9只），颈静脉采血后进行阉割处理。ELISA检测血样中睾酮（T）、瘦素、胰岛素及胰岛素样生长因子Ⅰ（IGF-Ⅰ）的浓度；免疫组化法检测睾丸组织中PPARGC1A/NRF1/TFAM通路相关蛋白的表达定位；RT-qPCR和Western blot检测睾丸组织中PPARGC1A/NRF1/TFAM通路相关基因与蛋白的表达变化。[结果] 与3月龄湖羊相比，9月龄湖羊外周血中T、瘦素、胰岛素及IGF-Ⅰ的浓度显著升高（P<0.05）。免疫组化法检测发现PPARGC1A、NRF1和TFAM蛋白在3月龄和9月龄湖羊睾丸组织的多种细胞内均呈时空特异性表达。此外，与3月龄湖羊相比，9月龄湖羊睾丸组织中PPARGC1A、NRF1和TFAM mRNA和蛋白表达水平均显著升高（P<0.05）。[结论] PPARGC1A/RNF1/TFAM通路相关基因可能与湖羊睾丸发育和性成熟过程密切相关。
Abstract:
[Objectives] The objective of this study was to elucidate the localization and expression of the key PPARGC1A/NRF1/TFAM pathway genes in the testis of prepubertal(3-month-old) and postpubertal(9-month-old) Hu sheep.[Methods] Eighteen male Hu sheeps with good body conditions and clear pedigree were selected for this experiment(3-and 9-month-old,n=9). The testis was collected after blood collection. The concentrations of testosterone(T),leptin,insulin and insulin-like growth factorⅠ(IGF-Ⅰ) in the blood samples was detected by ELISA;cellular localization of PPARGC1A/NEF1/TFAM pathway key proteins in testis was detected by immunohistochemistry;and the expression patterns of the PPARGC1A/NRF1/TFAM pathway core genes in testis tissue were detected by RT-qPCR and Western blot.[Results] Compared with the 3-month-old Hu sheep,the concentrations of the T,leptin,insulin,and IGF-Ⅰ in the peripheral blood of 9-month-old Hu sheep significantly increased(P<0.05,respectively). Immunohistochemistry results showed an age-related and tissue-specific expressions of PPARGC1A/RNF1/TFAM pathway key proteins in the testis of 3-month-old and 9-month-old Hu sheep. In addition,the mRNA of PPARGC1A,NRF1 and TFAM and its protein expressions in the testis of 9-month-old Hu sheep were significantly higher than those in 3-month-old Hu sheep(P<0.05).[Conclusions] The PPARGC1A/RNF1/TFAM pathway may be closely associated with testicular development and sexual maturation of Hu sheep.

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备注/Memo

备注/Memo:
收稿日期:2018-6-28。
基金项目:江苏现代农业（肉羊）产业技术体系项目[JATS（2018）276]；中国博士后科学基金项目（2015M581817）
作者简介:安世钰,硕士研究生。
通信作者:王锋,教授,从事肉羊营养繁殖调控研究,E-mail:caeet@njau.edu.cn。
更新日期/Last Update: 1900-01-01