[1]杨利国,张爱菊,郝易风,等.母牛注射PMSG及其抗血清后血清孕酮含量和PMSG的残留量[J].南京农业大学学报,1990,13(01):87-91.[doi:10.7685/j.issn.1000-2030.1990.01.016]
 Yang Liguo,Zhang Aiju,Hao Yifeng,et al.CONCENTRATIONS OF SERUM PROGESTERONE AND PMSG IN COWS TREATED WITH PMSG AND ANTI-PMSG SERUM[J].Journal of Nanjing Agricultural University,1990,13(01):87-91.[doi:10.7685/j.issn.1000-2030.1990.01.016]
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母牛注射PMSG及其抗血清后血清孕酮含量和PMSG的残留量()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
13卷
期数:
1990年01期
页码:
87-91
栏目:
出版日期:
1990-02-28

文章信息/Info

Title:
CONCENTRATIONS OF SERUM PROGESTERONE AND PMSG IN COWS TREATED WITH PMSG AND ANTI-PMSG SERUM
作者:
杨利国张爱菊郝易风朱章伦林耀星
南京农业大学家畜繁殖研究室 ,徐州市多管局 ,徐州市家畜良种站 ,江苏睢宁县多管局
Author(s):
Yang Liguo 1Zhang Aiju 2Hao Yifeng 3Zhu Zhanglin Lin Yiaoxing 4
1Laboratory of Animal Reproduction,Nanjing Agricultural University;2Xuzhou Municipal Management Bureau of Diversified Economy;3Xuzhou Municipal Animal Breeding Station;4Management Sub-bureau of Diversified Economy in Suinin Conuty
关键词:
黄牛 超数排卵 PMSG 抗血清 激素测定 酶免疫
Keywords:
cattle superovulation anti-PMSG serum hormone detections EIA
DOI:
10.7685/j.issn.1000-2030.1990.01.016
文献标志码:
A
摘要:
20头母黄牛在发情周期第12天注射孕马血清促性腺激素(PMSG)3000IU,2天后注射15-甲基-前列腺素 F_{2α}3mg,于发情当天注射兔抗 PMSG 血清2.4ml。应用酶免疫方法检测 PMSG 处理前和处理后笫48,84和240小时母牛血清孕酮含量和 PMSG 的残留量。结果表明:PMSG 在黄牛体内的半衰期长;根据PMSG 处理后第10天的血清孕酮水平可以预测母黄牛的超数排卵效果。
Abstract:
Twenty Chinese indigenous breed cattle cows with normal oestrous cycles were treatedwith 3 000 IU of pregnant mare′s serum gonadotrophin (PMSG) on day 12 of the cycle and 3mg of 15-methyl-prostaglandin F_{2α} on day 14,followed by 2.4 ml of rabbit anti-PMSG se-rum on the day of oestrus.The levels of progesterone and PMSG were detected in serum ofcows at the time of PMSG administration and at hours 48,84 and 240 after PMSG treat-ment by enzymeimmunoassay (EIA).The results showed that (1) there was a longer half-lifelength for PMSG in cows and (2) the EIA of serum progesterone on day 10 after PMSGtreatment could be applied to estimate the effect of superovulation in cows

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备注/Memo

备注/Memo:
国家自然科学基金
更新日期/Last Update: 1990-02-28