[1]彭萱,樊庆笙.高产β-葡聚精酶菌种分离方法的改进及其新菌种鉴[J].南京农业大学学报,1994,17(3):65-68.[doi:10.7685/j.issn.1000-2030.1994.03.010]
 Peng Xuan,Fan Qingsheng.THE IMPROVEMENT OF HIGH β-GLUCANASE PRODUCING STRAIN’S SELECTION AND IDENTIFICATION OF ITS STRAIN[J].Journal of Nanjing Agricultural University,1994,17(3):65-68.[doi:10.7685/j.issn.1000-2030.1994.03.010]
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高产β-葡聚精酶菌种分离方法的改进及其新菌种鉴()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
17卷
期数:
1994年3期
页码:
65-68
栏目:
出版日期:
1994-08-30

文章信息/Info

Title:
THE IMPROVEMENT OF HIGH β-GLUCANASE PRODUCING STRAIN’S SELECTION AND IDENTIFICATION OF ITS STRAIN
作者:
彭萱樊庆笙
南京农业大学自然资源与环境科学系
Author(s):
Peng Xuan Fan Qingsheng
Dept of Natural Resources, Environment Science, Nanjing Agric Univ, Nanjing 210095
关键词:
枯草芽孢杆菌 刚果红 β-葡聚糖 β-葡聚糖酶活性 透明圈 筛选
Keywords:
Bacillus subtilis Congo-red β-glucan β-glucanase activity transparent zone selection
分类号:
Q939.11
DOI:
10.7685/j.issn.1000-2030.1994.03.010
文献标志码:
A
摘要:
根据刚果红与β-1,3-1,4-葡聚糖紧密结合的特性,采用改进的方法筛选β-葡聚糖酶产生菌。凡菌落周围刚果红褪色的透明圈大者,其酶活性亦高。从12个土壤样品分离的上百个菌株中筛选,获得一株高产β-葡聚糖酶活性的菌株Bacillussubtilis9126,它在刚果红平皿上生长菌落周围的透明圈很大,其产酶活力高达57U/ml.
Abstract:
β-glucanase producing strain was selected by the improved method according to the strong interaction of β-1,3-1,4-glucan with Congo-red.The bigger the transparent zone arround the colony on plates containing Congored,the higher activity of β-glucanase.A high β-glucanase producing Bacillus strain 9126 was selected from hundreds of cultures isolated from 12 soil samples.This selected strain produced a large transparent zone around its colony.It produced β-glucanase as high as 57U/ml.

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更新日期/Last Update: 1994-08-30