[1]刘清,周光宏.离体小肠粘膜细胞对类胡萝卜素的吸收[J].南京农业大学学报,1997,20(4):54-59.[doi:10.7685/j.issn.1000-2030.1997.04.011]
 Liu Qing,Zhou Guanghong.UPTAKE OF CAROTENOIDS BY INTESTINAL MUCOUS CELLS OF LAYERS AND GOATS IN VITRO[J].Journal of Nanjing Agricultural University,1997,20(4):54-59.[doi:10.7685/j.issn.1000-2030.1997.04.011]
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离体小肠粘膜细胞对类胡萝卜素的吸收()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
20卷
期数:
1997年4期
页码:
54-59
栏目:
出版日期:
1997-11-30

文章信息/Info

Title:
UPTAKE OF CAROTENOIDS BY INTESTINAL MUCOUS CELLS OF LAYERS AND GOATS IN VITRO
作者:
刘清周光宏
南京农业大学动物科技学院
Author(s):
Liu Qing Zhou Guanghong
College of Animal Science & Technology, Nanjing Agric Univ, Nanjing 210095
关键词:
蛋鸡 山羊 β-胡萝卜素 黄体素 体外吸收
Keywords:
layers goats βcarotene lutein uptake in vitro
分类号:
S852.2
DOI:
10.7685/j.issn.1000-2030.1997.04.011
文献标志码:
A
摘要:
选用两种有代表性的类胡萝卜素(β-胡萝卜素和黄体素)分别用蛋鸡、山羊的离体小肠细胞进行体外吸收实验。结果表明:β-胡萝卜素和黄体素的吸收均呈浓度依赖性,且β-胡萝卜素吸收率保持稳定(P>0.05);培养温度对蛋鸡离体小肠粘膜细胞类胡萝卜素吸收无显著影响,而极显著地影响了山羊对黄体素和高浓度(≥15μmol/L)β-胡萝卜素的吸收;Tween20对两种类胡萝卜素均有抑制作用,其中对黄体素抑制作用较大;体积分数(φ)为0.3%的Tween20可能阻断了β-胡萝卜素转化为维生素A;0.5mol/L的根皮素抑制了β-胡萝卜素的吸收而对黄体素吸收起促进作用(P<0.01)。
Abstract:
Uptake of βcarotene and lutein by layers and goats was studied in vitro. The result showed uptake of βcarotene and lutein was concentrationdependent and a constant uptake percentage of βcarotene was observed. This suggested carotenoids were uptaken by passive diffusion mechanism. Uptake of carotenoids by cells of layer was temperatureindependent while uptake of lutein and βcarotene at high concentration by goats was affected significantly by incubation temperature. Tween 20 inhibited uptake of carotenoids among which lutein was more heavily inhibited than βcarotene. In addition inhibition of βcarotene and promotion of lutein by phloretin were first reported.

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备注/Memo

备注/Memo:
国家教委回国人员基金
更新日期/Last Update: 1997-11-30