[1]沈文飚,徐朗莱,胡蕴珠,等.大豆花叶病毒(SMV)的侵染对大豆叶片过氧化物酶及其同功酶的影响[J].南京农业大学学报,1997,20(4):88-92.[doi:10.7685/j.issn.1000-2030.1997.04.018]
 Shen Wenbiao,Xu Langlai,Hu Yunzhu,et al.EFFECTS OF SMVINFECTION ON PEROXIDASE AND ITS ISOZYMES OF SOYBEAN LEAVES[J].Journal of Nanjing Agricultural University,1997,20(4):88-92.[doi:10.7685/j.issn.1000-2030.1997.04.018]
点击复制

大豆花叶病毒(SMV)的侵染对大豆叶片过氧化物酶及其同功酶的影响()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
20卷
期数:
1997年4期
页码:
88-92
栏目:
出版日期:
1997-11-30

文章信息/Info

Title:
EFFECTS OF SMVINFECTION ON PEROXIDASE AND ITS ISOZYMES OF SOYBEAN LEAVES
作者:
沈文飚徐朗莱胡蕴珠智海剑孔军礼
南京农业大学理学院
Author(s):
Shen Wenbiao Xu Langlai Hu Yunzhu Zhi Haijian Sun Junli
College of Sciences,  2Soybean Research Institute, Nanjing Agric Univ, Nanjing 210095
关键词:
大豆 大豆花叶病毒 过氧化物酶 同功酶 等电聚焦
Keywords:
soybean soybean mosaic virus (SMV) peroxidase isozyme isoelectrofocusing
分类号:
S435.651
DOI:
10.7685/j.issn.1000-2030.1997.04.018
文献标志码:
A
摘要:
研究了对SMV抗性不同的3个大豆品种在接种SMV-a株系后叶片过氧化物酶活性及其同功酶的动态变化。结果表明,侵染的早期(接种后3d),抗病品种科丰1号的过氧化物酶活性急剧上升,出现了新的PI为4.40和9.73的同功酶;感病品种徐豆1号和1138-2的变化则相对较小。侵染的中后期(接种后6d和9d),感病品种的过氧化物酶活性迅速上升,且徐豆1号也出现了PI为4.40和9.73的同功酶;抗病品种过氧化物酶活性略有下降,PI为4.40和9.73的同功酶消失。SMV侵染早期,抗病品种过氧化物酶活性的急剧增加以及PI为4.40和9.73的同功酶的出现可能与抗病性密切相关。
Abstract:
Dynamic changes in activity and electrophoretogram of peroxidase were studied with leaves of resistant and susceptible soybean cultivars during infection by SMVa. The results suggested that peroxidase activities of resistant soybean cultivar (Kefeng1) increased rapidly, and two new isozymes of PI 4.40 and 9.73 appeared at the early stage of infection (three days after inoculation). On the other hand, the changes of peroxidase were relatively smaller in leaves of susceptible soybean cultivars (Xudou1 and 11382). At the middle and late stages of infection (six days and nine days after inoculation), peroxidase activities showed a greater increase in susceptible cultivars, and two identical isozymes of PI 4.40 and 9.73 in Xudou1 were found. While peroxidase activities of resistant soybean cultivar decreased slightly and the two new isozymes disappeared. In a word, rapid increment of peroxidase activities and appearance of two isozymes with PI 4.40 and 9.73 at the early stage of infection might be positively correlated with disease resistance to SMV in resistant cultivars.

相似文献/References:

[1]徐仲伟,徐克章*,张治安,等.吉林省不同年代大豆品种植株地上器官生物量的变化[J].南京农业大学学报,2011,34(3):7.[doi:10.7685/j.issn.1000-2030.2011.03.002]
 XU Zhong-wei,XU Ke-zhang,ZHANG Zhi-an,et al.Changes of biomass above-ground organs of soybean cultivars with year of release in Jilin Province[J].Journal of Nanjing Agricultural University,2011,34(4):7.[doi:10.7685/j.issn.1000-2030.2011.03.002]
[2]汪霞,徐宇,李广军,等.大豆株高QTL定位及Meta 分析[J].南京农业大学学报,2011,34(3):13.[doi:10.7685/j.issn.1000-2030.2011.03.003]
 WANG Xia,XU Yu,LI Guang-jun,et al.Mapping quantitative trait loci and Meta-analysis for plant height in soybean(Glycine max L.Merr.)[J].Journal of Nanjing Agricultural University,2011,34(4):13.[doi:10.7685/j.issn.1000-2030.2011.03.003]
[3]邴鑫,张治安,徐克章,等.不同叶运动能力大豆品种叶枕结构的比较研究[J].南京农业大学学报,2009,32(4):18.[doi:10.7685/j.issn.1000-2030.2009.04.004]
 BING Xin,ZHANG Zhi-an,XU Ke-zhang,et al.Comparative studies on structure of pulvinus in different capacity of leaf-movement soybean[J].Journal of Nanjing Agricultural University,2009,32(4):18.[doi:10.7685/j.issn.1000-2030.2009.04.004]
[4]赵洪祥,徐克章,李大勇,等.吉林省不同年代育成大豆品种硝酸还原酶活性变化及其与产量的关系[J].南京农业大学学报,2007,30(2):13.[doi:10.7685/j.issn.1000-2030.2007.02.003]
 ZHAO Hong-xiang,XU Ke-zhang,LI Dayong,et al.Studies on nitrate reductase activity and the relationship with yield in leaves of the soybean cultivars released in Jilin Province at different ages[J].Journal of Nanjing Agricultural University,2007,30(4):13.[doi:10.7685/j.issn.1000-2030.2007.02.003]
[5]赵团结,盖钧镒.大豆柱头外露突变体PSA-1的发现与遗传分析[J].南京农业大学学报,2006,29(4):15.[doi:10.7685/j.issn.1000-2030.2006.04.004]
 ZHAO Tuan-jie,GAI Jun-yi.Discovery and genetic analysis of a mutant with protruding stigma from anthers in soybean[J].Journal of Nanjing Agricultural University,2006,29(4):15.[doi:10.7685/j.issn.1000-2030.2006.04.004]
[6]徐德平,江汉湖,肖凯,等.大豆异黄酮的分离鉴定与抗氧化作用的研究[J].南京农业大学学报,2001,24(3):89.[doi:10.7685/j.issn.1000-2030.2001.03.022]
 Xu Deping,Jiang Hanhu,Xiao Kai,et al.Isolation,identification of isoflavones in soybean and their antioxidant abilities[J].Journal of Nanjing Agricultural University,2001,24(4):89.[doi:10.7685/j.issn.1000-2030.2001.03.022]
[7]邱家驯,赵团结,盖钧镒.中国大豆育成品种中苏沪地区种质的遗传贡献[J].南京农业大学学报,1997,20(4):1.[doi:10.7685/j.issn.1000-2030.1997.04.001]
 Qiu Jiaxun,Zhao Tuanjie,Gai Junyi.THE GENETIC CONTRIBUTION OF THE GERMPLASM FROM JIANGSU AND SHANGHAI TO SOYBEAN CULTIVARS RELEASED DURING 1923-1995 IN CHINA[J].Journal of Nanjing Agricultural University,1997,20(4):1.[doi:10.7685/j.issn.1000-2030.1997.04.001]
[8]盖钧镒,邱家驯,赵团结.大豆品种南农493-1和南农1138-2与其衍生新品种的亲缘关系及其育种价值分析[J].南京农业大学学报,1997,20(1):1.[doi:10.7685/j.issn.1000-2030.1997.01.001]
 Gai Junyi,Qiu Jiaxun,Zhao Tuanjie.AN ANALYSIS OF GENETIC RELATIONSHIP OF NANNONG493 1 AND NANNONG 1138 2 WITH THEIR DERIVATIVE CULTIVARS AND THEIR POTENTIAL IN FUTURE BREEDING[J].Journal of Nanjing Agricultural University,1997,20(4):1.[doi:10.7685/j.issn.1000-2030.1997.01.001]
[9]夏基康,浦奉华,张建.大豆抗豆秆黑潜蝇性能的研究 Ⅳ.豆秆蝇各虫态历期和不同日龄幼虫的特征与蛀害习性[J].南京农业大学学报,1986,9(01):23.[doi:10.7685/j.issn.1000-2030.1986.01.004]
 Xia Jikang,Pu Fonghua,Zhang Jian.STUDIES ON THE RESISTANCE OF SOYBEAN TO THE SOYBEAN AGROMYZID FLY, MELANAGROMYZA SOJAE Ⅳ.LIFE STAGES,DAY STADIUM AND FEEDING CHARACTER OF MAGGOT[J].Journal of Nanjing Agricultural University,1986,9(4):23.[doi:10.7685/j.issn.1000-2030.1986.01.004]
[10]陈永萱,薛宝娣,张凤如,等.蚜虫的消长与春大豆花叶病毒病的田间流行[J].南京农业大学学报,1988,11(01):60.[doi:10.7685/j.issn.1000-2030.1988.01.011]
 Chen Yongxuan,Xiu Baodi,Zhang Fengru,et al.APHID FLIGHT ACTIVITY AND EPIDEMIOLOGY OF SMV IN THE SPRING PLANTED SOYBEAN FIELD[J].Journal of Nanjing Agricultural University,1988,11(4):60.[doi:10.7685/j.issn.1000-2030.1988.01.011]
[11]程利国,李广军,刘玉芹,等.大豆对大豆花叶病毒Sa株系抗扩展特性的遗传分析[J].南京农业大学学报,2009,32(4):13.[doi:10.7685/j.issn.1000-2030.2009.04.003]
 CHENG Li-guo,LI Guang-jun,LIU Yu-qin,et al.Genetic analysis of resistance of soybean (Glycine max (L.) Merr.) to soybean mosaic virus strain Sa[J].Journal of Nanjing Agricultural University,2009,32(4):13.[doi:10.7685/j.issn.1000-2030.2009.04.003]

备注/Memo

备注/Memo:
国家自然科学基金
更新日期/Last Update: 1997-11-30