[1]江昌俊,李叶云,王朝霞.茶树鲜叶中β-葡萄糖苷酶提取条件的研究[J].南京农业大学学报,2000,23(2):93-96.[doi:10.7685/j.issn.1000-2030.2000.02.023]
 Jiang Changjun,Li Yeyun,Wang Zhaoxia.Study on the extraction conditions of β?glucosidase in fresh leaves of tea plant[J].Journal of Nanjing Agricultural University,2000,23(2):93-96.[doi:10.7685/j.issn.1000-2030.2000.02.023]
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茶树鲜叶中β-葡萄糖苷酶提取条件的研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
23卷
期数:
2000年2期
页码:
93-96
栏目:
出版日期:
2000-05-30

文章信息/Info

Title:
Study on the extraction conditions of β?glucosidase in fresh leaves of tea plant
作者:
江昌俊李叶云王朝霞
安徽农业大学农业部茶叶生物技术重点开放实验室,合肥,230036
Author(s):
Jiang Changjun Li Yeyun Wang Zhaoxia
Key Lab of Tea Biotechnology, Ministry of Agriculture, Anhui Agric Univ, Hefei 230036
关键词:
茶树 鲜叶 β-葡萄糖苷酶 提取条件 酶活性
Keywords:
tea plant fresh leaf β?glucosidase extracting conditions enzyme activity
分类号:
TS272
DOI:
10.7685/j.issn.1000-2030.2000.02.023
文献标志码:
A
摘要:
研究了从茶树鲜叶中提取 β 葡萄糖苷酶时不溶性聚乙烯吡咯烷酮 (polyvinypolypyrrolidone ,PVPP)的加入量、缓冲液的选择、多酚类浓度对酶活性的影响及酶的部分特性。结果表明 :加入与鲜叶重量相等的PVPP可获得较高的酶活性 ;选用 0 1mol/L (离子强度为 0 7)的柠檬酸—柠檬酸钠缓冲液 (pH 6 0 )作为提取缓冲液可得到活性较高的酶液 ;浓度仅为 4mg/L的茶叶多酚类物质能使酶完全失活 ;β 巯基乙醇和EDTA对酶活性有抑制作用 ;而甘油能提高酶活性 ,2~2 5mol/L的甘油效果较明显 ;NaCl对酶活性几乎没有影响
Abstract:
Effects of amount of PVPP, different buffers and polyphenol concentration on the β?glucosidase activity were studied when the enzymes were extracted from fresh leaves of tea plant. The results showed that a suitable amount of PVPP for extracting enzyme is equal to that of fresh leaves. The enzyme presented higher activity with 0 1 mol/L(ion strength 0 7)citrate buffer(pH 6 0)comparatively, but tea polyphenol(4 mg/L)inhibited the enzyme activity completely, β?mercaptoethanol and EDTA could also inhibit its activity. However glycerol was beneficial to its stability and could obviously protect its activity, especially in 2?2 5 mol/L. The β?glucosidase activity was little affected by NaCl.

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备注/Memo

备注/Memo:
安徽省国际科技合作项目(99258009)
更新日期/Last Update: 2000-05-30