[1]欧瑜,陆承平.检测猪链球菌2型毒力相关蛋白的ELISA法的建立[J].南京农业大学学报,2001,24(2):94-97.[doi:10.7685/j.issn.1000-2030.2001.02.023]
 Ou Yu,Lu Chengping.Establishing of ELISA for detecting virulence related proteins of Streptococcus suis type 2[J].Journal of Nanjing Agricultural University,2001,24(2):94-97.[doi:10.7685/j.issn.1000-2030.2001.02.023]
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检测猪链球菌2型毒力相关蛋白的ELISA法的建立()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
24卷
期数:
2001年2期
页码:
94-97
栏目:
出版日期:
2001-05-30

文章信息/Info

Title:
Establishing of ELISA for detecting virulence related proteins of Streptococcus suis type 2
作者:
欧瑜陆承平
南京农业大学农业部
Author(s):
Ou Yu Lu Chengping
Key Laboratory of Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agric Univ, Nanjing 210095, China
关键词:
猪链球菌 溶菌酶释放蛋白 胞外因子 酶联免疫吸附测定
Keywords:
Streptococcus suis type 2 muraminidase released protein extracellular facter enzyme linked immunosorbent assay
分类号:
S858.28
DOI:
10.7685/j.issn.1000-2030.2001.02.023
文献标志码:
A
摘要:
提纯猪链球菌 2型分离株HA980 1的毒力相关蛋白溶菌酶释放蛋白 (MRP)和胞外因子 (EF) ,制备其抗体。用此抗体建立了Dot ELISA和间接ELISA检测法。分别以菌株ATCC35 2 46和HA980 1为阴性与阳性对照 ,检测 17株猪源链球菌和 1株猪链球菌 2型人分离株。结果显示 ,两种ELISA的检测结果一致 ,MRP和EF的阳性率均为 61% (11/18)。
Abstract:
Virulence related proteins of Streptococcus suis type 2, muraminidase released protein(MRP)and extracellular facter(EF)were extracted from isolate HA9801 and were used as antigens for preparation of antibodies. Dot ELISA and indirect ELISA were established for detection MRP and EF. 17 strains swine Streptococcus and 1 human isolate of Streptococcus suis type 2 were detected, while strain ATCC35246 was used as negtive control and strain HA9801 as positive control. It showed that Dot ELISA and indirect ELISA appeared correspondent results. Positive rates of MRP and EF both were 61%.

参考文献/References:

[1] Vecht U,Wisselink H J,Jellema M L.Identification of two proteins associated with virulence of Streptococcus suis type 2[J].Infection and Immunity.1991,59(9):3156-3162
[2] Vecht U,Wisselink H J,Anakotta J.Discrimination between virulent and nonvirulent Streptococcus suis type 2 strains by enzyme-linked immunosorbent assay[J].Veterinary Microbiology.1993,34:71-82
[3] 姚火春,陈国强,陆承平.猪链球菌1998分离株病原特性鉴定[J].南京农业大学学报.1999,22(2):67-70
[4] Salasia S I O,Lammler Ch.Detection of virulence-associated proteins of Streptococcus suis by cultivation of the bacteria on nitrocellulose membranes[J].MEDICAL SCIENCE RESEARCH.1994,22:347-348
[5] Vecht U,Arends J P,van der Molen E J.Differences in virulence between two strains of Streptococcus suis type 2 after experimentally induced infection of newborn germ-free pigs[J].American Journal of Veterinary Research.1989,50(7):1037-1043

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备注/Memo

备注/Memo:
国家“973”项目子课题(G199901、906)
更新日期/Last Update: 2001-05-30