[1]韦朝领,江昌俊,陶汉之,等.茶树紫黄素脱环氧化酶基因的cDNA 克隆及其生物信息学分析[J].南京农业大学学报,2003,26(1):14-19.[doi:10.7685/j.issn.1000-2030.2003.01.004]
 WEI Chao ling,JIANG Chang jun,TAO Han zhi,et al.Cloning and bioinformatics analysis of sequence signature of violaxanthin de-epoxidase cDNA in tea plant ( Camellia sinensis (L.) O.Kuntzes )[J].Journal of Nanjing Agricultural University,2003,26(1):14-19.[doi:10.7685/j.issn.1000-2030.2003.01.004]
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茶树紫黄素脱环氧化酶基因的cDNA 克隆及其生物信息学分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
26卷
期数:
2003年1期
页码:
14-19
栏目:
出版日期:
2003-02-28

文章信息/Info

Title:
Cloning and bioinformatics analysis of sequence signature of violaxanthin de-epoxidase cDNA in tea plant ( Camellia sinensis (L.) O.Kuntzes )
作者:
韦朝领江昌俊陶汉之宛晓春
安徽农业大学农业部茶叶生物技术重点开放实验室,安徽,合肥,230036
Author(s):
WEI Chao ling JIANG Chang jun TAO Han zhi WAN Xiao chun
Key Laboratory of Tea Biotechnology, Ministry of Agriculture, Anhui Agric Univ, Hefei 230036, China
关键词:
茶树 紫黄素脱环氧化酶 cDNA 生物信息学 叶黄素循环
Keywords:
tea plant(Camellia sinensis (L )) violaxanthin de epoxidase cDNA bioinformatics xanthophyll cycle
分类号:
Q943
DOI:
10.7685/j.issn.1000-2030.2003.01.004
文献标志码:
A
摘要:
根据GenBank中已登录的植物紫黄素脱环氧化酶 (VDE)基因序列 ,以其高度保守的氨基酸序列设计一对简并引物 ,采用RT PCR与 3′/ 5′RACE相结合的方法 ,从茶树 (Camelliasinensi)中克隆出VDE的全长cDNA ,长度为 16 32bp。分析表明 ,该序列的 5′端和 3′端的非编码序列长度分别为 6 1bp和 14 0bp ,开放阅读框为 14 2 2bp ,编码 4 73个氨基酸 ,其转运肽长度为 132个氨基酸。成熟蛋白的氨基酸序列与拟南芥、莴苣、菠菜及烟草的同源性分别为 83 5 % ,82 7% ,82 1%和 83 6 % ,且有 3个特殊的结构域 :半胱氨酸富集区、脂肪结合蛋白特征区和由谷氨酸富集的高电荷区。分析表明 ,茶树VDE具有热稳定性。
Abstract:
In order to clone a complete cDNA of violaxanthin de epoxidases(VDE) from young leaves of Camellia sinensis, a pair of degenerate primers were designed according to the high conserved amino acid sequences in GenBank, and RT PCR, 3′/5′ RACE and full length PCR were used Analysis of the cloned complete cDNA, which has 1 632 bp, showed that it encompassed a open reading frame(ORF) with 1 422 bp ecoding 473 amino acid (aa) residues, including 132 aa transit peptide and 341 aa mature polypeptide Alignments with the deduced amino acid of mature proteins in four species of high plants show 83 5%, 82 7%, 82 1% and 83 6%, identity with Arabidopsis thaliana, Lactuca sativa, Spinacia oleracea and Nicotiana tabacum, respectively. Meanwhile, the mature VDE protein in tea plant has a cysteine rich domain, a lipocalin signature and a high glutamate rich domain and the feature of thermal stability

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备注/Memo

备注/Memo:
农业部农业结构调整重大技术研究专项(20021202A);安徽省教育厅自然基金资助项目(2000JL067)
更新日期/Last Update: 2003-02-28