[1]陈迪新,张绍铃,陶书田.沙梨花粉原位萌发与花粉管生长特性[J].南京农业大学学报,2004,27(3):34-37.[doi:10.7685/j.issn.1000-2030.2004.03.008]
 CHEN Di xin,ZHANG Shao ling,TAO Shu tian.Characteristics of pollen germination and pollen tube growth in in vivo sand pear styles[J].Journal of Nanjing Agricultural University,2004,27(3):34-37.[doi:10.7685/j.issn.1000-2030.2004.03.008]
点击复制

沙梨花粉原位萌发与花粉管生长特性()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
27卷
期数:
2004年3期
页码:
34-37
栏目:
出版日期:
2004-08-30

文章信息/Info

Title:
Characteristics of pollen germination and pollen tube growth in in vivo sand pear styles
作者:
陈迪新张绍铃陶书田
南京农业大学园艺学院,江苏,南京,210095
Author(s):
CHEN Di xin ZHANG Shao ling TAO Shu tian
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
自花授粉 异花授粉 花粉萌发 花粉管生长
Keywords:
pear self-pollination cross-pollination pollen germination pollen tube growth
分类号:
S661.2
DOI:
10.7685/j.issn.1000-2030.2004.03.008
文献标志码:
A
摘要:
用荧光显微镜对自交不亲和强度不同的梨品种丰水和菊水自花授粉及以丰水为母本的异花授粉后的花粉萌发及花粉管生长特性进行观察。结果表明 ,自花与异花花粉在柱头上均有较高的萌发率 ,高达 95 %以上 ,且二者间没有显著的差异。自花与异花花粉管在花柱内的生长速度及停止生长的位置不同 ,丰水自花花粉管生长到花柱 1/ 3处就完全停止生长 ,表现为强自交不亲和性 ,而菊水自花花粉管大部分也在花柱 1/ 3处停止生长 ,但仍有少量长至花柱基部 ,表现为弱自交不亲和性 ;而异花授粉的花粉管虽然在花柱内也有停止生长现象 ,但大量的花粉管能长到花柱基部 ,表现为杂交亲和性。花柱内自花授粉的花粉管在停止生长之前有弯曲、先端膨大变形等现象 ,而异花授粉的花粉管没有此现象
Abstract:
Two pear cultivars with different self incompatible strength, Housui and Kikusui, were self pollinated and Housui was pollinated with the pollen of Kikusui, respectively. Following characteristics of pollen germination and pollen tube growth were observed with fluorescence microscope. Both the self and cross pollinated pollen grains on the stigma had a high percentage of pollen germination that was up to over 95%, and there was no distinct difference between them. However, the speed of pollen tube growth and the place where pollen tube growth arrested of both in in vivo style were different. The pollen tube growth of Housui self pollinated completely arrested at the upper one third of the style, which behaved strong self incompatibility. While the arrest of pollen tube growth of Kikusui self pollinated most occurred at the upper one third of the style, only some pollen tubes still grew to the style bottom, which made Kikusui behave as a weakly self incompatible cultivar. Although some cross pollinated pollen tube growth arrested in the styles, lots of pollen tubes still grew to the style bottom, which behaved cross compatibility. The self pollinated pollen tubes in the styles often followed curve, swell and front deformation before arresting growth, but not the cross pollinated ones in the styles.

参考文献/References:

[1] De Nettancourt D.Incompatibility and Incongruity in Wild and Cultivated Plants[M].Berlin:Springer-Verlag.2001:1-25
[2] McCbbin B A,Kao T H.Molecular recognition and response in pollen-pistil interactions[J].Annual Review of Cell and Developmental Biology.2000,16:333-364
[3] McClure B A,Gray J E,Anderson M A.S-locus products in Nicotiana alata pistils are subject to organ-specific post-transcriptional processing,but not post-transcriptional processing[J].Plant Molecular biology.1990,22:177-187
[4] Kho Y O,Bear J.Observing pollen tubes by means of fluorescence[J].Euphytica.1968,17:298-302
[5] Graaf B H J,Derksen J W M,Mariani C.Pollen and pistil in the progamic phase[J].SEXUAL PLANT REPRODUCTION.2001,14:41-45
[6] Lush W M,Clarke A E.Observations of pollen tube growth in Nicotiana alata and their implications for the mechanism of self-incompatibility[J].SEXUAL PLANT REPRODUCTION.1997,10:27-35
[7] Suwan T,John N.Pollen viability and pollen tube growth following controlled pollination and their relation to low fruit production in teak(Tectona grandis L .f.)[J].Annals of Botany.1997,80:401-410
[8] Zhang S L,Hiratsuka S.Variations in S-protein levels in styles of Japanese pears and the expression of self-incompatibility[J].Journal of the Japanese Society for Horticultural Science.1999,68(5):911-918

相似文献/References:

[1]申春苗,汪良驹,王文辉,等.12个梨品种果实冰点温度的检测与影响因素分析[J].南京农业大学学报,2011,34(1):35.[doi:10.7685/j.issn.1000-2030.2011.01.007]
 SHEN Chun-miao,WANG Liang-ju,WANG Wen-hui,et al.Determination of the freezing point temperature of 12 pear cultivars and the correlation analysis of the impact factors[J].Journal of Nanjing Agricultural University,2011,34(3):35.[doi:10.7685/j.issn.1000-2030.2011.01.007]
[2]路娟,吴俊,张绍铃,等.不同系统梨种质遗传多样性与分类关系的SSR分析[J].南京农业大学学报,2011,34(2):38.[doi:10.7685/j.issn.1000-2030.2011.02.008]
 LU Juan,WU Jun,ZHANG Shao-ling,et al.Genetic diversity and polygentic relationship among pears reveled by SSR analysis[J].Journal of Nanjing Agricultural University,2011,34(3):38.[doi:10.7685/j.issn.1000-2030.2011.02.008]
[3]沙守峰,李俊才,张绍铃,等.‘新苹梨’果实和叶片发育期有机酸含量的变化及其相关性研究[J].南京农业大学学报,2011,34(6):41.[doi:10.7685/j.issn.1000-2030.2011.06.008]
 SHA Shou-feng,LI Jun-cai,ZHANG Shao-ling,et al.Correlation analysis of changes in the contents of organic acid in developing fruit and leaf of ‘Xinping pear’[J].Journal of Nanjing Agricultural University,2011,34(3):41.[doi:10.7685/j.issn.1000-2030.2011.06.008]
[4]王鑫,吴华清,陶书田,等.大棚和露地栽培条件下梨果实发育特性的差异[J].南京农业大学学报,2012,35(2):27.[doi:10.7685/j.issn.1000-2030.2012.02.005]
 WANG Xin,WU Hua-qing,TAO Shu-tian,et al.Difference of pear fruit development feature between plastic tunnel and open field[J].Journal of Nanjing Agricultural University,2012,35(3):27.[doi:10.7685/j.issn.1000-2030.2012.02.005]
[5]申明,成学慧,谢荔,等.氨基酸叶面肥对砂梨叶片光合作用的促进效应[J].南京农业大学学报,2012,35(2):81.[doi:10.7685/j.issn.1000-2030.2012.02.014]
 SHEN Ming,CHENG Xue-hui,XIE Li,et al.Effects of amino-acid fertilizers on photosynthesis in leaves of pear cultivars[J].Journal of Nanjing Agricultural University,2012,35(3):81.[doi:10.7685/j.issn.1000-2030.2012.02.014]
[6]宋晓晖,谢凯,李艳丽,等.HPLC-ELSD法测定梨果实中不同种类可溶性糖含量[J].南京农业大学学报,2012,35(2):87.[doi:10.7685/j.issn.1000-2030.2012.02.015]
 SONG Xiao-hui,XIE Kai,LI Yan-li,et al.Determination of contents and components of water-soluble sugar in pear fruit by HPLC-ELSD[J].Journal of Nanjing Agricultural University,2012,35(3):87.[doi:10.7685/j.issn.1000-2030.2012.02.015]
[7]陈慧,张树军,张妤艳,等.40个梨品种S基因型的鉴定及S基因频率分析[J].南京农业大学学报,2013,36(5):21.[doi:10.7685/j.issn.1000-2030.2013.05.004]
 CHEN Hui,ZHANG Shujun,ZHANG Yuyan,et al.Identification of S-genotypes in forty pear cultivars and analysis of S-RNase genes frequency in Pyrus[J].Journal of Nanjing Agricultural University,2013,36(3):21.[doi:10.7685/j.issn.1000-2030.2013.05.004]
[8]李芳芳,张绍铃,张虎平,等.不同梨品种花粉量及花粉萌发率差异研究[J].南京农业大学学报,2013,36(5):27.[doi:10.7685/j.issn.1000-2030.2013.05.005]
 LI Fangfang,ZHANG Shaoling,ZHANG Huping,et al.Cluster analysis for the quantity and germinating characteristics of the pollens from different pear cultivars[J].Journal of Nanjing Agricultural University,2013,36(3):27.[doi:10.7685/j.issn.1000-2030.2013.05.005]
[9]王超,张全军,黄小三,等.‘金坠’梨成花相关基因PbLFY、PbTFL1和PbFT的克隆及时空表达模式分析[J].南京农业大学学报,2014,37(2):26.[doi:10.7685/j.issn.1000-2030.2014.02.005]
 WANG Chao,ZHANG Quanjun,HUANG Xiaosan,et al.Cloning and temporal and spatial expression pattern of flowering-related genes PbLFY,PbTFL1 and PbFT in‘Jinzhui’pear[J].Journal of Nanjing Agricultural University,2014,37(3):26.[doi:10.7685/j.issn.1000-2030.2014.02.005]
[10]赵碧英,刘妮,田瑞,等.梨品种资源果实萼片及萼洼性状评价[J].南京农业大学学报,2014,37(4):53.[doi:10.7685/j.issn.1000-2030.2014.04.008]
 ZHAO Biying,LIU Ni,TIAN Rui,et al.Evaluation on calyx characters of Pyrus pyrifolia fruit[J].Journal of Nanjing Agricultural University,2014,37(3):53.[doi:10.7685/j.issn.1000-2030.2014.04.008]

备注/Memo

备注/Memo:
国家自然科学基金资助项目(30170651,30270926)
更新日期/Last Update: 2004-08-30