[1]钱春桃,陈劲枫,罗向东.黄瓜抗枯萎病异源易位植株AT-04的鉴定筛选[J].南京农业大学学报,2006,29(2):20-24.[doi:10.7685/j.issn.1000-2030.2006.02.005]
 QIAN Chun-tao,CHEN Jin-feng,LUO Xiang-dong.Identification and characterization of cucumber alien translocation plant AT-04 with resistance to fusarium wilt[J].Journal of Nanjing Agricultural University,2006,29(2):20-24.[doi:10.7685/j.issn.1000-2030.2006.02.005]
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黄瓜抗枯萎病异源易位植株AT-04的鉴定筛选()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
29卷
期数:
2006年2期
页码:
20-24
栏目:
出版日期:
2006-05-30

文章信息/Info

Title:
Identification and characterization of cucumber alien translocation plant AT-04 with resistance to fusarium wilt
作者:
钱春桃陈劲枫罗向东
南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095
Author(s):
QIAN Chun-tao CHEN Jin-feng LUO Xiang-dong
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
关键词:
黄瓜 染色体 枯萎病
Keywords:
cucumber chromosome fusarium wilt
分类号:
S642.2
DOI:
10.7685/j.issn.1000-2030.2006.02.005
文献标志码:
A
摘要:
通过对黄瓜种间杂交后代BC1F4群体进行农艺学和细胞学分析,鉴定筛选出抗枯萎病异源易位植株AT 04。农艺学观察表明,其具有小叶、短果、棕色瘤刺、多分枝的特点,形态上偏向原始亲本野生种Cucum is hystrixChakr.,育性与栽培黄瓜相近。细胞学研究发现,AT 04在有丝分裂的中后期至少有1对染色体的姊妹染色单体分离明显滞后。在减数分裂终变期,平均每个花粉母细胞有0.2个四价体、0.05个六价体和0.05个八价体,约30%的花粉母细胞中存在拉十字的染色体。在中期Ⅰ,7个二价体出现分组分布现象,主要以3+2+2形式存在,表明其至少经历了4次染色体易位。田间调查和人工接种鉴定均表明,易位植株AT 04及其自交后代和野生种C.hystrix对枯萎病具有较高抗性。
Abstract:
Agronomic and cytological methods were combined in identification and characterization of an alien translocation plant(AT04)among plants of population BC_1F_4 that was derived from the interspecific hybridization between Cucumis savitus L. and C. hystrix Chakr. . In morphology, the plant AT04 was characterized to have small-sized leaf, short fruit, brown spine and multi-branching habit, which were closely similar to those of the original parent wild species(C. hystrix), and to have a fertility as high as that of cultivated cucumber. Cytological observation in AT04 showed that sister chromatids in at least one pair of somatic chromosomes were delayed obviously in separation at late metaphase. At diakinesis, 0.2 tetravalents, 0.05 hexavalents and 0.05 octovalents per pollen mother cell(PMC)were detected, which was absent in cultivated cucumber. In 30% PMCs, crossed chromosomes occurred at diakinesis. At metaphase Ⅰ, 7 bivalents were frequently detected to separate into three groups, with a form of 3+2+2, which indicated that at least four translocation events once occurred. Results from both field investigation on plants and artificial inoculation on seedlings of AT04 and its progenies from selfing confirmed their resistance to fusarium wilt as observed in the wild species C. hystrix.

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备注/Memo

备注/Memo:
国家863计划项目(2004AA241120);国家自然科学基金资助项目(30470120);南京农业大学青年科技创新基金项目(KJ04009)
更新日期/Last Update: 2006-05-30