[1]季镭,王金成,杨秀丽,等.三种茎线虫rDNA区的PCR-RFLP分析[J].南京农业大学学报,2006,29(3):39-43.[doi:10.7685/j.issn.1000-2030.2006.03.008]
 JI Lei,WANG Jin-cheng,YANG Xiu-li,et al.PCR-RFLP patterns for differentiation of three Ditylenchus species[J].Journal of Nanjing Agricultural University,2006,29(3):39-43.[doi:10.7685/j.issn.1000-2030.2006.03.008]
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三种茎线虫rDNA区的PCR-RFLP分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
29卷
期数:
2006年3期
页码:
39-43
栏目:
出版日期:
2006-08-30

文章信息/Info

Title:
PCR-RFLP patterns for differentiation of three Ditylenchus species
作者:
季镭王金成杨秀丽黄国明林茂松
南京农业大学植物保护学院,江苏,南京,210095;天津出入境检验检疫局,天津,300456
Author(s):
JI Lei WANG Jin-cheng YANG Xiu-li HUANG Guo-ming LIN Mao-song
College of Plant Protection, Nanjing Agricultural Uiniversity, Nanjing 210095, China; Tianjin Entry-Exit Inspection and Qurantine Bureau, Tianjin 300456, China
关键词:
甘薯茎线虫 鳞球茎茎线虫 食菌茎线虫 ITS-RFLP 酶切图谱
Keywords:
Ditylenchus destructor D.dipsaci D.myceliophagus ITS-RFLP enzyme-cutting pattern
分类号:
S433
DOI:
10.7685/j.issn.1000-2030.2006.03.008
文献标志码:
A
摘要:
采用PCR-RFLP技术对来自国内外的10个茎线虫属群体进行了限制性片段长度多态性分析(RFLP),发现甘薯茎线虫(D itylenchus destructor)不同群体间存在基因分化现象。来自山东的2个群体Dell和Dely的酶切图谱与来自其他地区的线虫群体相比明显不同。对甘薯茎线虫的近似种鳞球茎茎线虫(D.dipsaci)以及食菌茎线虫(D.myceliophagus)的分析结果显示,通过ITS-RFLP技术建立的酶切图谱可以很好地将3种茎线虫区分开来。
Abstract:
The internal transcribed spacer regions of 10 populations of Ditylenchus were analyzed using PCR-RFLP techniques. The results showed that two ITS-RFLP patterns were found in Ditylenchus destructor and 2 populations of D. destructor from Shandong Province, Dell and Dely, had unique enzyme-digestion pattern compared to other populations of this species. The study also supported that PCR-ITS-RFLP method was a valuable tool for differentiation of D. destructor, D. dipsaci and D. myceliophagus.

参考文献/References:

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备注/Memo

备注/Memo:
天津自然科学基金资助项目(05YFJMJC10800)
更新日期/Last Update: 2006-08-30