[1]宗成文,章镇,房经贵,等.葡萄LEAFY基因启动子的克隆与序列分析[J].南京农业大学学报,2007,30(4):20-25.[doi:10.7685/j.issn.1000-2030.2007.04.005]
 ZONG Cheng-wen,ZHANG Zhen,FANG Jing-gui,et al.Cloning and sequence analysis of LEAFY gene promoter from grape(Vitis vinifera×V.labrusca)[J].Journal of Nanjing Agricultural University,2007,30(4):20-25.[doi:10.7685/j.issn.1000-2030.2007.04.005]
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葡萄LEAFY基因启动子的克隆与序列分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
30卷
期数:
2007年4期
页码:
20-25
栏目:
出版日期:
2007-11-30

文章信息/Info

Title:
Cloning and sequence analysis of LEAFY gene promoter from grape(Vitis vinifera×V.labrusca)
作者:
宗成文章镇房经贵陶建敏赵密珍
南京农业大学园艺学院,江苏,南京,210095;延边大学农学院,吉林,龙井,133400;江苏省农业科学院园艺研究所,江苏,南京,210014
Author(s):
ZONG Cheng-wen ZHANG Zhen FANG Jing-gui TAO Jian-min ZHAO Mi-zhen
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; College of Agriculture, Yanbian University, Longjing 133400, China; Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
葡萄 LEAFY基因 启动子 克隆 序列分析
Keywords:
grape LEAFY gene promoter cloning sequence analysis
分类号:
S663.1
DOI:
10.7685/j.issn.1000-2030.2007.04.005
文献标志码:
A
摘要:
为探明葡萄LEAFY基因的表达调控规律,应用PCR技术从藤稔葡萄中克隆了1个长1833 bp的DNA片段,该序列含有2个内含子区域,编码402个氨基酸,与葡萄LEAFY同源基因VFL有99%的同源性。应用基因组步移法克隆了LEAFY基因的5′侧翼序列925 bp,拼接后的LEAFY基因及启动子序列共2 692 bp(GenBank登录号EF222286)。用PLACE、P lantCARE在线启动子预测工具分析表明:该序列含有启动子的特定结构,如TATA-box,CAAT-box等,另外含有一些顺式作用元件如MYB结合位点、ABA响应元件、光响应元件和一些其他的调控序列,说明葡萄LEAFY基因的表达可能受MYB、ABA和光等的调控。用FootPrinter在线工具对葡萄与拟南芥等其他4种植物的LEAFY同源基因启动子进行比较,发现不同植物的启动子既有保守性,又有多样性,转录因子结合位点的分布相似,但也有区别,暗示了LEAFY基因表达调控的精确性或多样性。
Abstract:
In order to study the rule of grape LEAFY gene expression and regulation, a fragment from grape cultivar’Fujiminori’was amplified by PCR. It was 1 833 bp in length, deduced 402 amino acids, had two introns and 99% identity with grape LEAFY homologue VFL. 925 bp promoter region sequence was cloned by genome walking method. The gene and promoter were 2 692 bp in length(GenBank accession EF222286). Promoter sequence analyzed by PLACE and PlantCARE showed that it had TATA-box, CAAT-box and some cis-acting element such as MYB binding site, cis-acting regulatory element involved in ABA response, some light-induced responsive elements and other transcription factor-binding sites. Compared the promoter of grape LEAFY homologue with other 4 plants by FootPrinter analysis, these promoter had conservatism and diversity and the distributing of transcription factor-binding sites had similarity and difference. It implied the accuracy and diversity of LEAFY gene expression and regulation.

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备注/Memo

备注/Memo:
江苏省科技厅高新技术项目(BG2007309);江苏省农业资源开发局科技项目(2007KJ-22);上海市科技兴农重点攻关项目(沪农科攻字2004第1-8-1);江苏省农业三项工程项目(sx(2007)033)
更新日期/Last Update: 2007-11-30