[1]李晶,蒋明义,张阿英,等.H2O2参与低水势下ABA维持玉米初生根的生长[J].南京农业大学学报,2008,31(1):6-10.[doi:10.7685/j.issn.1000-2030.2008.01.002]
 LI Jing,JIANG Ming-yi,ZHANG A-ying,et al.H_2O_2 is required for abscisic acid-regulated maize primary root growth at low water potentials[J].Journal of Nanjing Agricultural University,2008,31(1):6-10.[doi:10.7685/j.issn.1000-2030.2008.01.002]
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H2O2参与低水势下ABA维持玉米初生根的生长()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
31卷
期数:
2008年1期
页码:
6-10
栏目:
出版日期:
2008-02-28

文章信息/Info

Title:
H_2O_2 is required for abscisic acid-regulated maize primary root growth at low water potentials
作者:
李晶蒋明义张阿英桑建荣
南京农业大学生命科学学院,江苏,南京,210095
Author(s):
LI Jing JIANG Ming-yi ZHANG A-ying SANG Jian-rong
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
玉米 初生根 低水势 脱落酸 H2O2
Keywords:
maize primary root low water potentials abscisic acid(ABA) H2O2
分类号:
S513
DOI:
10.7685/j.issn.1000-2030.2008.01.002
文献标志码:
A
摘要:
以玉米(Zea maysL.)杂交种农大108为材料,以PEG 6000(25%)模拟低水势,研究H2O2是否参与低水势下ABA维持玉米初生根的生长。结果发现在低水势下,经ABA抑制剂10μmol.L-1fluridone(FLU)和1 mmol.L-1tung-state(Tun)预处理,显著抑制了玉米初生根的生长,加入外源100μmol.L-1ABA后,恢复了初生根的生长。利用透射电镜观察,发现在低水势下,维持生长的玉米初生根部有少量H2O2积累,用H2O2的清除剂二甲基硫脲(dimethythio-urea,DMTU)5 mmol.L-1、碘化钾(KI)30 mmol.L-1和丙酮酸钠(Na-Pyruvate,Na-pyr)30 mmol.L-1预处理并在低水势下培养,发现玉米的初生根生长几乎完全被抑制。上述结果表明:在低水势下,玉米初生根通过积累ABA维持生长,少量的HO参与了低水势下ABA维持玉米初生根生长的过程,且不可缺少。
Abstract:
Maize(Zea mays L. ’Nongda 108’, from Nanjing Agricultural University, China)was used in this experiment and 25% PEG 6000 was used to simulate low water potentials(-1.6 MPa). The objective of this experiment was to determine whether H2O2 is involved in abscisic acid(ABA)maintained primary root growth at low water potentials. At low water potentials, seedlings were pretreated with the ABA inhibitors fluridone(FLU, 10 μmol·L-1)and tungstate(Tun, 1 mmol·L-1), both of them largely inhibited the primary root elongation, and the addition of 100 μmol·L-1 ABA almost fully prevented the inhibition of root elongation. Transmission electron microscopy was used to find that a little amount of H2O2 accumulated in the maize primary root at low water potentials. Using the scavengers of H2O2 to pretreat the seedlings and then exposing them to low water potentials, the primary root elongation was largely inhibited. In summary, these results show that ABA maintains maize primary root growth at low water potentials and H2O2 is involved in this process. If all the H2O2 is removed at low water potentials, the elongation of the primary root will be greatly inhibited.

参考文献/References:

[1] Zhu J K.Salt and drought stress signal transduction in plants[J].Annual Review of Plant Biology.2002,53:247-273
[2] Ober E S,Sharp R E.Proline accumulation in maize(Zea mays L.)primary roots at low water potentials.Ⅰ.Requirement for increased levels of abscisic acid[J].Plant Physiology.1994,105:981-987
[3] Jiang M Y,Zhang J H.Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves[J].Journal of Experimental Botany.2002,53:2401-2410
[4] Bestwick C S,Brown I R,Bennett M H R.Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv.phaseolicola[J].Plant Cell.1997,9:209-221
[5] Cutler A J,Krochko J E.Formation and breakdown of ABA[J].Trends in Plant Science.1999,4:472-478
[6] Liotenberg S,North H,Marion-Poll A.Molecular biology and regulation of abscisic acid biosynthesis in plants[J].Plant Physiology and Biochemistry.1999,37:341-350
[7] Polle A.Dissecting the superoxide dismutase-ascorbate glutathione-pathway in chloroplasts by metabolic modeling.Computer simulations as a step towards flux analysis[J].Plant Physiology.2001,126:445-462
[8] Hammond-Kosack K E,Jones J D G.Resistance gene-dependent plant defense responses[J].Plant Cell.1996(8):1773-1791
[9] Asada K.The water-water cycle in chloroplasts:scavenging of active oxygens and dissipation of excess photons[J].Annual Review of Plant Physiology and Plant Molecular Biology.1999,50:601-609
[10] Sharp R E,Poroyko V,Hejlek L G.Root growth maintenance during water deficits:physiology to functional genomics[J].Journal of Experimental Botany.2004,55:2343-2351
[11] van Breusegem F,Vranová E,Dat J F.The role of active oxygen species in plant signal transduction[J].Plant Science.2001,161:405-414
[12] Mittler R.Oxidative stress,antioxidants and stress tolerance[J].Trends in Plant Science.2002,7:405-411
[13] Overmyer K,Brosché M,Kangasj(a)rvi J.Reactive oxygen species and hormonal control of cell death[J].Trends in Plant Science.2003,8:335-342
[14] Liszkay A,van der Zalm E,Schopfer P.Production of reactive oxygen intermediates(,H2O2 and@OH)by maize root and their role in wall loosening and elongation growth[J].Plant Physiology.2004,136:3114-3123
[15] Foreman J,Demidchik V,Bothwell J H F.Reactive oxygen species produced by NADPH oxidase regulate plant cell growth[J].Nature.2003,422:442-446
[16] Guan L M,Zhao J,Scandalios J G.Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress:H2O2 is the likely intermediary signaling molecule for the response[J].Plant Journal.2000,22:87-95
[17] Saab I N,Sharp R E,Pritchard J.Increased endogenous abscisic acid maintains primary root growth and inhibits shoot growth of maize seedlings at low water potentials[J].Plant Physiology.1990,93:1329-1336

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备注/Memo

备注/Memo:
教育部高等学校博士点基金(20040307011)
更新日期/Last Update: 2008-02-28