[1]苏世鸣,任丽轩,杨兴明,等.西瓜专化型尖孢镰刀菌的分离鉴定及水稻根系分泌物对其生长的影响[J].南京农业大学学报,2008,31(1):57-62.[doi:10.7685/j.issn.1000-2030.2008.01.012]
 SU Shi-ming,REN Li-xuan,YANG Xing-ming,et al.Identification of the Fusarium oxysporum f.sp. niveum and the effects of rice root exudates on its growth[J].Journal of Nanjing Agricultural University,2008,31(1):57-62.[doi:10.7685/j.issn.1000-2030.2008.01.012]
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西瓜专化型尖孢镰刀菌的分离鉴定及水稻根系分泌物对其生长的影响()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
31卷
期数:
2008年1期
页码:
57-62
栏目:
出版日期:
2008-02-28

文章信息/Info

Title:
Identification of the Fusarium oxysporum f.sp. niveum and the effects of rice root exudates on its growth
作者:
苏世鸣任丽轩杨兴明徐阳春周俊沈其荣
南京农业大学资源与环境科学学院,江苏,南京,210095
Author(s):
SU Shi-ming REN Li-xuan YANG Xing-ming XU Yang-chun ZHOU Jun SHEN Qi-rong
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
关键词:
西瓜 水稻 尖孢镰刀菌 根系分泌物
Keywords:
watermelon(Citrullus lanatus) rice Fusarium oxysporum root exudates
分类号:
S436.5
DOI:
10.7685/j.issn.1000-2030.2008.01.012
文献标志码:
A
摘要:
利用尖孢镰刀菌选择性培养基从西瓜枯萎病发病部位分离出病原菌,通过Booth′s镰刀菌分类鉴定标准和专化型试验,确定该菌株为西瓜专化型尖孢镰刀菌(Fusarium oxysporum f.sp. niveum)。以该菌株为材料,研究水稻根系分泌物对其生长的影响。结果表明,2个水稻品种ELIO和4007的根系分泌物均能显著抑制其孢子萌发和孢子产生。当根系分泌物的加入量为1.5 mL时,ELIO和4007对孢子萌发的抑制率分别为37.8%和41.0%;当加入20 mL水稻根系分泌物时,ELIO和4007对孢子产生的抑制率达到96.3%和76.4%。水稻根系分泌物对西瓜尖孢镰刀菌孢子产生过程的抑制作用主要发生在孢子萌发阶段和孢子在尖孢镰刀菌瓶状小梗上的生成阶段,而对菌丝营养生长没有影响。西瓜与旱作水稻间作的盆栽试验表明,间作系统中西瓜苗的鲜重和株高分别提高了186.0%和80.5%。单作西瓜枯萎病发病率为66.7%,死亡率为44.4%,而间作处理中西瓜均生长正常。间作条件下,西瓜根际土壤中的尖孢镰刀菌数量显著降低,与单作根际土壤相比减少了91%。表明:水稻根系分泌物能显著抑制西瓜专化型尖孢镰刀菌生长,采用旱作水稻与西瓜间作能有效缓解西瓜枯萎病的发生。
Abstract:
The pathogen of wilt disease was isolated from the infected watermelon plants using selective medium. According to the symptoms, morphology and pathogenicity, the pathogen was identified as Fusarium oxysporum f. sp. niveum. Rice root exudates of both ELIO and 4007 were collected to investigate the effects of them on the spore development of F. oxysporum f. sp. niveum. The absolute value of influence ratio to spore germination of F. oxysporum f. sp. niveum was 37.8% and 41.0%, with adding 1.5 mL root exudates of ELIO and 4007, respectively. The absolute value of influence ratio to F. oxysporum f. sp. niveum reproduction reached 96.3% and 76.4%, with adding 20 mL root exudates of ELIO and 4007, respectively. Pot experiments were carried out to investigate the effect of intercropping with aerobic rice on Fusarium wilt in watermelon. The results showed that fresh weight and plant height of watermelon seedlings increased by 186.0% and 80.5% in intercropping system, respectively, compared with those in mono-cropping system. 66.7% of watermelon were infected with wilt disease and 44.4% died after 40 days of transplanting in mono-cropped soil, but those were normally grown in intercropping system. The density of F. oxysporum decreased 91% in intercropped rhizosphere soil compared with mono-cropped soil after 40 days of transplanting. It is suggested that intercropping with aerobic rice alleviated Fusarium wilt in watermelon, by restraining the spore reproduction and spore germination of F. oxysporum f. sp. niveum through the production of rice root exudates.

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备注/Memo

备注/Memo:
科技部973项目(2007CB109304);农业部948项目(2006-G62);南京农业大学青年科技创新基金项目(KJ04006)
更新日期/Last Update: 2008-02-28