[1]刁卫平,陈劲枫,雷春,等.影响黄瓜未授粉子房培养胚发生因素的研究[J].南京农业大学学报,2008,31(1):137-140.[doi:10.7685/j.issn.1000-2030.2008.01.028]
 DIAO Wei-ping,CHEN Jin-feng,LEI Chun,et al.Factors affecting embryo formation in unpollinated ovary culture of cucumber[J].Journal of Nanjing Agricultural University,2008,31(1):137-140.[doi:10.7685/j.issn.1000-2030.2008.01.028]
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影响黄瓜未授粉子房培养胚发生因素的研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
31卷
期数:
2008年1期
页码:
137-140
栏目:
出版日期:
2008-02-28

文章信息/Info

Title:
Factors affecting embryo formation in unpollinated ovary culture of cucumber
作者:
刁卫平陈劲枫雷春宋慧张晓青
南京农业大学作物遗传与种质创新国家重点实验室/园艺学院,江苏,南京,210095
Author(s):
DIAO Wei-ping CHEN Jin-feng LEI Chun SONG Hui ZHANG Xiao-qing
State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
黄瓜 未授粉子房 胚发生率
Keywords:
cucumber unpollinated ovary embryo formation frequency
分类号:
S642.2
DOI:
10.7685/j.issn.1000-2030.2008.01.028
文献标志码:
A
摘要:
以不同基因型黄瓜为试材,研究了子房发育时期、热激处理时间、TDZ和AgNO3对黄瓜未授粉子房培养过程中胚发生的影响。结果表明:处于开花前2~3 d的子房胚发生率相对较高,达83.8%;在子房培养开始阶段进行35℃处理的胚发生率显著高于对照(0 d),其中处理3 d的效果最好;添加0.01~0.04 mg.L-1TDZ的诱导培养基培养的胚发生率为20.0%~72.7%,其中添加0.04 mg.L-1TDZ的胚发生效果较好;在诱导培养基中添加AgNO3可以提高胚发生率,同时能缩短胚出现的时间并提高胚产量。
Abstract:
Different genotypes of cucumber were used to study the effects of development stage of ovaries, time of thermal treatment, and concentration of TDZ and AgNO3 on the unpollinated ovary culture of cucumber. The results showed that the highest frequency 83.8% of embryo formation was obtained as using ovaries 2-3 d before anthesis. Embryo formation after thermal shock treatment at 35 ℃ was significantly higher than control(0 d), and highest embryo formation was found in treating three days. Embryo formation frequencies were from 20.0% to 72.7% when TDZ was added from 0.01-0.04 mg·L-1 to inducting medium, and the best result was obtained at 0.04 mg·L-1 TDZ. Adding AgNO3 to inducting medium could increase embryo formation frequency, and shorten the time for embryo sprouting and enhance the embryo yields.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金项目(30470120,30671419);教育部高等学校博士点基金项目(20050307009);江苏省高新技术研究计划项目(BG2007301)
更新日期/Last Update: 2008-02-28