[1]成妍,班青宇,王倩,等.不结球白菜游离小孢子培养及再生植株的倍性鉴定[J].南京农业大学学报,2009,32(2):25-29.[doi:10.7685/j.issn.1000-2030.2009.02.006]
 CHENG Yan,BAN Qing-yu,WANG Qian,et al.Isolated microspore culture and ploidy identification of microspore-derived plants in Brassica campestris ssp.chinensis[J].Journal of Nanjing Agricultural University,2009,32(2):25-29.[doi:10.7685/j.issn.1000-2030.2009.02.006]
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不结球白菜游离小孢子培养及再生植株的倍性鉴定()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
32卷
期数:
2009年2期
页码:
25-29
栏目:
出版日期:
2009-05-30

文章信息/Info

Title:
Isolated microspore culture and ploidy identification of microspore-derived plants in Brassica campestris ssp.chinensis
作者:
成妍班青宇王倩侯喜林
南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095
Author(s):
CHENG Yan BAN Qing-yu WANG Qian HOU Xi-lin
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
关键词:
不结球白菜 小孢子培养 再生植株 倍性鉴定
Keywords:
Brassica campestris ssp.chinensis microspore culture regenerated plants ploidy identification
分类号:
S634.3
DOI:
10.7685/j.issn.1000-2030.2009.02.006
文献标志码:
A
摘要:
对影响不结球白菜小孢子培养和植株再生的几个因素及再生植株的倍性进行分析。结果表明:基因型是影响小孢子胚诱导率的最重要因子,正反交材料的胚诱导率无显著差异;供体植株开花后6~10 d培养可获得高的胚诱导率;琼脂在8~12 g.L-1范围内,随着质量浓度的提高可显著提高小孢子胚再生率。结合流式细胞仪和植株形态鉴定,不结球白菜小孢子再生植株自然加倍率总体水平较高,有的高达90%,但不同基因型间有明显差异。
Abstract:
Several factors affecting isolated microspore culture of non-heading Chinese cabbage were studied. The results showed that the genotype was the most important factor on the inducing rate of microspore-derived embryos, and no significant difference was observed between reciprocal cross. The best inflorescence age of the donor plants was 6-10 d after bloom. Higher agar concentration from 8 g·L-1 to 12 g·L-1 could significantly promote the regeneration of embryos. Based on the flow cytometry and identification of the morphological characteristics, a high level of the spontaneous doubling efficiency from microspore-derived plants was observed in non-heading Chinese cabbage, even to 90%, and there were obvious differences between genotypes.

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备注/Memo

备注/Memo:
国家863计划项目(2006AA100108)
更新日期/Last Update: 2009-05-30