[1]刘利锋,张军忍,覃少华,等.硫酸头孢喹肟在猪体内的药动学研究[J].南京农业大学学报,2009,32(2):102-106.[doi:10.7685/j.issn.1000-2030.2009.02.021]
 LIU Li-feng,ZHANG Jun-ren,QIN Shao-hua,et al.Studies on cefquinome sulphate for injectable suspension and its pharmacokinetics in swine[J].Journal of Nanjing Agricultural University,2009,32(2):102-106.[doi:10.7685/j.issn.1000-2030.2009.02.021]
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硫酸头孢喹肟在猪体内的药动学研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
32卷
期数:
2009年2期
页码:
102-106
栏目:
出版日期:
2009-05-30

文章信息/Info

Title:
Studies on cefquinome sulphate for injectable suspension and its pharmacokinetics in swine
作者:
刘利锋张军忍覃少华赵杰黄业咸江善祥
南京农业大学动物医学院,江苏,南京,210095;海安县大公兽医站,江苏,海安,226623
Author(s):
LIU Li-feng ZHANG Jun-ren QIN Shao-hua ZHAO Jie HUANG Ye-xian JIANG Shan-xiang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; Dagong Veterinary Station of Hai’an, Hai’an 226623, China
关键词:
硫酸头孢喹肟 药动学 SPE-HPLC
Keywords:
cefquinome sulphate pharmacokinetics SPE-HPLC swine
分类号:
S859
DOI:
10.7685/j.issn.1000-2030.2009.02.021
文献标志码:
A
摘要:
建立猪血浆中硫酸头孢喹肟的SPE-HPLC测定方法,对硫酸头孢喹肟在猪体内的药动学特征进行研究。选用健康猪6头,进行单次肌肉注射2 mg.kg-1自制的2.5%硫酸头孢喹肟注射液,提取血浆药物经SPE柱净化后进行HPLC分析。色谱条件为C18反相柱,流动相:高氯酸钠-磷酸-三乙胺缓冲液(pH3.6)与乙腈混合液(体积比为90∶10)。结果显示,硫酸头孢喹肟在0.05~25μg.mL-1线性关系良好(R2=0.9986),最低检测限为0.05μg.mL-1。经3p97软件分析结果显示:硫酸头孢喹肟的药时数据适合一级吸收二室模型,主要药动学参数:tmax为(0.80±0.05)h,Cmax为(2.44±0.24)μg.mL-1,AUC为(7.28±1.11)μg.mL-1.h,T1/2β为(2.28±0.49)h。结果提示:健康猪单次肌肉注射硫酸头孢喹肟后吸收迅速,持效时间较长,临床用药18 h后仍能维持对常见病原菌的有效血药浓度。建议临床用药:2 mg.kg-1(以头孢喹肟计),1日1次。
Abstract:
SPE-HPLC method was established to determine cefquinome sulphate, and to study the pharmacokinetics of cefquinome sulphate in swine plasma. Cefquinome sulphate were administrated i. m. at a dose of 2 mg·kg-1 to six swine. After extracted with methanol, cefquinome sulphate concentration of preparation samples were determined by HPLC/UV with solid phase extraction method. C18 was used as the revesed column, sodium perchlorate-phosphoric acid buffer(pH 3.6):acetonitrile(90:10, V/V) as the mobile phate, the concentration-time date were analyzed with 3p97 computer program. The results indicat that the standard curves for cefquinome is linar in a range of 0.05-25 μg·mL-1(R2=0.998 6), the limit of detection was 0.05 μg·mL-1;the results suggest that the kinetics of the injection is fit to two compartment model with first order absorption. The main parameters of cefquinome sulphate is tmax=(0.80±0.05)h, Cmax=(2.44±0.24)μg·mL-1, AUC=(7.28±1.11)μg·mL-1·h, T1/2β=(2.28±0.49)h. We conclud that cefquinome sulphate could be absorbed rapidly, long persistence effective time, there is enough effect after 18 h by i. m. cefquinome sulphate. 2 mg·kg-1 and 1 day 1 time is adviced.

参考文献/References:

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备注/Memo

备注/Memo:
国家自然科学基金项目(30771626)
更新日期/Last Update: 2009-05-30