[1]程利国,李广军,刘玉芹,等.大豆对大豆花叶病毒Sa株系抗扩展特性的遗传分析[J].南京农业大学学报,2009,32(4):13-17.[doi:10.7685/j.issn.1000-2030.2009.04.003]
 CHENG Li-guo,LI Guang-jun,LIU Yu-qin,et al.Genetic analysis of resistance of soybean (Glycine max (L.) Merr.) to soybean mosaic virus strain Sa[J].Journal of Nanjing Agricultural University,2009,32(4):13-17.[doi:10.7685/j.issn.1000-2030.2009.04.003]
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大豆对大豆花叶病毒Sa株系抗扩展特性的遗传分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
32卷
期数:
2009年4期
页码:
13-17
栏目:
出版日期:
2009-11-30

文章信息/Info

Title:
Genetic analysis of resistance of soybean (Glycine max (L.) Merr.) to soybean mosaic virus strain Sa
作者:
程利国李广军刘玉芹李河南章元明
南京农业大学作物遗传与种质创新国家重点实验室/国家大豆改良中心,江苏 南京 210095;临沂师范学院,山东 临沂 276005;临沂市农业科学院,山东 临沂 276012
Author(s):
CHENG Li-guo LI Guang-jun LIU Yu-qin LI He-nan ZHANG Yuan-ming
State Key Laboratory of Crop Genetics and Germplasm Enhancement/National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing 210095, China; Linyi Normal College, Linyi 276005, China; Linyi Academy of Agricultural Sciences, Linyi 276012, China
关键词:
大豆 大豆花叶病毒 数量性状基因座 SSR 遗传图谱
Keywords:
soybean soybean mosaic virus quantitative trait locus simple sequence repeats genetic linkage map
分类号:
S565.1
DOI:
10.7685/j.issn.1000-2030.2009.04.003
文献标志码:
A
摘要:
作物抗性遗传研究可为抗性育种提供理论基础。在溧水中子黄豆×南农493-1杂交组合的244个F2∶3家系中,随机取171个F2∶3家系为材料,用150对SSR分子标记,通过JoinMap3.0软件构建了包括70对分子标记的25个连锁群;采用平均病级和综合病情指数两种指标,用Win QTL Cartographer V2.5软件的多区间作图法进行QTL定位。结果表明:大豆对大豆花叶病毒Sa株系的抗扩展平均病级和综合病情指数均有4个QTL,其中在C2-b连锁群的satt422-satt640标记间和D2-a连锁群有共同的QTL,两性状的4个和5个互作QTL可分别解释表型变异的15.14%和52.6%。这些结果为抗性性状的遗传剖析和标记辅助育种提供理论依据。
Abstract:
The genetic analysis for resistance traits is the foundation for resistance breeding in crop. One hundred and seventy-one F2:3 lines were randomly selected from 244 F2:3 lines derived from the cross between Lishuizhongzihuangdou and Nannong 493-1 and measured for resistance of soybean(Glycine max(L. )Merr. )to soybean mosaic virus(SMV)strain Sa, with two indicators including average disease degree and synthetic disease index. Among 150 SSR loci, a linkage map with twenty-five linkage groups was constructed with 70 SSR loci, covering 1 123.99 cM. Therefore, an attempt was made to detect quantitative trait loci(QTL)for the two traits above by using multiple interval mapping in the software of Win QTL Cartographer V2.5.The results showed that 4 QTLs were detected for each of the two traits above. Among these QTLs, 2 common QTL were identified, one is between satt422 and satt640 on the linkage group C2-b and the other is on the linkage group D2-a;and a total of 4 and 5 epistatic QTLs with 15.14% and 52.6% of the total phenotypic variance explained were detected for the two traits above, respectively. The results presented here will enhance the understanding of the genetic basis of the resistant traits and provide a solid theoretical foundation for soybean marker-assisted selection.

参考文献/References:

[1]濮祖芹,房德纯. 大豆花叶病毒的株系鉴定[J]. 植物保护学报, 1982
[2]杨雅麟. 长江中下游地区大豆花叶病毒(SMV)株系组成、分布及抗性研究[D]. 南京:南京农业大学, 2002
[3]战勇. 黄淮地区大豆花叶病毒的生物学检测、株系鉴定及大豆抗性的遗传与基因定位[D]. 南京:南京农业大学, 2003
[4]东方阳. 大豆对SMV株系抗性的遗传分析和RAPD标记研究[D]. 南京:南京农业大学, 1999
[5]智海剑. 大豆对大豆花叶病毒抗侵染和抗扩展特性的鉴定、遗传和利用研究[D]. 南京:南京农业大学, 2005
[6]刘丽君,高明杰. 大豆对SMV1株系抗性基因的分子标记研究[J]. 大豆科学, 2002
[7]栾晓燕,满为群. 与大豆SMV3号株系抗性相关的分子标记的鉴定[J]. 分子植物育种, 2006
[8]滕卫丽,邱丽娟. 大豆SMV 3号株系抗病基因的SSR标记[J]. 大豆科学, 2005
[9]王永军,王修强. 大豆5个花叶病毒株系抗性基因的定位[J]. 遗传学报, 2004
[10]王永军,喻德跃. 重组自交系群体的检测调整方法及其在大豆NJRIKY群体的应用[J]. 作物学报, 2004
[11]张志永,盖钧镒. 大豆花叶病毒抗性基因Rsa的分子标记[J]. 科学通报, 1998
[12]郑翠明. 大豆对SMV3号株系的抗性遗传及分子标记研究[D]. 北京:中国农业科学院, 2000
[13]Imsande,M,Shoemaker,R,Shoemaker,R. QTL in Soybase:A new perspective[J]. Soybean Genetics Newsletter, 1998
[14]Jeong,S,C,Hayes,A,J,Hayes,A,J. Genetic and sequence analysis of markers tightly linked to the soybean mosaic virus resistance gene,Rsv3[J]. Crop Science, 2002
[15]Saghai-Maroof,M,A,Jorgensen,R,A,Jorgensen,R,A. Ribisomal DNA space-length polymorphism in barley:mendelian inheritance,chromosomal location,and population dynamics[J]. Proceedings of the National Academy of Sciences(USA), 1984
[16]Stam,P. Construction of integrated genetic linkage maps by means of a new computer package:JoinMap[J]. Plant Journal, 1993
[17]Wang,S,Zeng,Z,B,Zeng,Z,B. Windows QTL Cartographer V25[OL]http://statgenncsuedu/qtlcart/WQTLCarthtm, 2006
[18]Yu,Y,G,Buss,G,R,Buss,G,R. RFLP and microsatellite mapping of a gene for soybean mosaic virus resistance[J]. Phytopathology, 1994
[19]李海朝. 大豆对大豆花叶病毒的抗性遗传与SSR标记定位研究[D]. 南京:南京农业大学, 2006
[20]Hayes,A,J,Buss,G,R,Buss,G,R. Molecular marker mapping of Rsv4,a gene conferring resistance to all known strains of soybean mosaic virus[J]. Crop Science, 2000
[21]宛煜嵩. 大豆遗传图谱的构建及若干农艺性状的QTL定位分析[D]. 北京:中国农业科学院, 2002
[22]Cregan,P,B,Bush,A,L,Bush,A,L. An integrated genetic linkage map of the soybean genome[J]. Crop Science, 1999
[23]Song,Q,J,Shoemaker,R,C,Shoemaker,R,C. A new integrated genetic 1inkage map of the soybean[J]. Theoretical and Applied Genetics, 2004
[24]Zhang,Y,M. Advances on methods for mapping QTL in plant[J]. Chinese Science Bulletin, 2006

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备注/Memo

备注/Memo:
国家自然科学基金项目(30470998);教育部新世纪优秀人才支持计划项目(NCET-05-0489);教育部高等学校博士点基金项目(20060307008);教育部“111”项目(B08025)
更新日期/Last Update: 2009-11-30