[1]魏跃,陈啸寅,李振陆,等.黄瓜6磷酸葡萄糖酸脱氢酶基因cDNA片段的克隆及表达分析[J].南京农业大学学报,2010,33(1):37-42.[doi:10.7685/j.issn.1000-2030.2010.01.008]
 WEI Yue CHEN Xiao-yin,LI Zhen-lu,WANG Yong-ping,et al.Cloning of 6-phosphogluconate dehydrogenase gene cDNA fragments from cucumber and expression analysis[J].Journal of Nanjing Agricultural University,2010,33(1):37-42.[doi:10.7685/j.issn.1000-2030.2010.01.008]
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黄瓜6磷酸葡萄糖酸脱氢酶基因cDNA片段的克隆及表达分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
33卷
期数:
2010年1期
页码:
37-42
栏目:
出版日期:
2010-01-31

文章信息/Info

Title:
Cloning of 6-phosphogluconate dehydrogenase gene cDNA fragments from cucumber and expression analysis
作者:
魏跃陈啸寅李振陆王永平史建磊吴志明张蜀宁陈劲枫
南京农业大学农业部南方蔬菜遗传改良重点开放实验室,江苏,南京,210095;江苏农林职业技术学院,江苏,镇江,212400
Author(s):
WEI Yue CHEN Xiao-yin LI Zhen-lu WANG Yong-ping SHI Jian-lei WU Zhi-ming ZHANG Shu-ning CHEN Jin-feng
Key Laboratory of Southern Vegetable Crop Genetics Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; Jiangsu Polytechnical College of Agriculture and Forestry, Zhenjiang 212400, China
关键词:
黄瓜 6-磷酸葡萄糖酸脱氢酶 cDNA 克隆
Keywords:
Cucumis sativus L. 6PGDH cDNA clone
分类号:
S642.2
DOI:
10.7685/j.issn.1000-2030.2010.01.008
文献标志码:
A
摘要:
以黄瓜品种露丰为材料,根据已报道的6-磷酸葡萄糖酸脱氢酶基因(6PGDH)的保守氨基酸序列设计简并引物,利用RT-PCR技术获得黄瓜6PGDH的cDNA同源片段,命名为CSPG(登录号为EU815934)。该片段长度为1 207 bp,包含一个936 bp的开放阅读框(编码311个氨基酸)和271 bp的Poly A 3′非翻译区末端,无内含子;该基因编码的氨基酸序列与拟南芥、大豆、水稻、玉米、菠菜的6PGDH基因有75%以上的同源性。运用半定量RT-PCR技术对6PGDH基因的转录水平进行分析,结果表明:该基因在叶、根、茎中均有表达,高温胁迫下的表达量高于常温对照,说明6PGDH基因与热胁迫相关。
Abstract:
With homology-based PCR strategy, a fragment cDNA named CSPG(accession number EU815934) was cloned from Cucumis sativus‘Lufeng’. The length of CSPG was 1 207 bp, which encompassed an open reading frame(ORF) with 936 bp encoding 311 amino acid residues and 271 bp non-translation 3’-terminal end region, and no intron existed in CSPG. The protein encoded by CSPG was more than 75%highly homologous 6-phosphogluconate dehydrogenase gene(6PGDH) of Arabidopsis thaliana, Glycine max, Oryza sativa, Zea mays, Spinacia oleracea. The expression of 6PGDH was analyzed by semi-quantitative RTPCR indicating that the gene had expression in leaf, root and stem, and that the amount of expression was higher in heat stress than normal temperature. Which showed that expression of 6PGDH was related to heat stress.

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备注/Memo

备注/Memo:
国家自然科学基金重点项目(30830079);国家自然科学基金项目(30671419,30700541);国家863专项经费项目(2008AA10Z150,2006AA100108,2006AA10Z108);教育部111计划项目(B08025)
更新日期/Last Update: 2010-01-31