[1]郑佳秋,侯喜林,朱红芳.热激诱导不结球白菜热激蛋白合成与耐冷性分析[J].南京农业大学学报,2010,33(2):30-34.[doi:10.7685/j.issn.1000-2030.2010.02.006]
 ZHENG Jia-qiu,HOU Xi-lin,ZHU hong-fang.Induction of heat shock protein synthesis and chilling tolerance in Brassica campestris ssp.chinensis[J].Journal of Nanjing Agricultural University,2010,33(2):30-34.[doi:10.7685/j.issn.1000-2030.2010.02.006]
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热激诱导不结球白菜热激蛋白合成与耐冷性分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
33卷
期数:
2010年2期
页码:
30-34
栏目:
出版日期:
2010-03-31

文章信息/Info

Title:
Induction of heat shock protein synthesis and chilling tolerance in Brassica campestris ssp.chinensis
作者:
郑佳秋侯喜林朱红芳
南京农业大学作物遗传与种质创新国家重点实验室/农业部南方蔬菜遗传改良重点开放实验室,江苏,南京,210095
Author(s):
ZHENG Jia-qiu HOU Xi-lin ZHU hong-fang
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetics Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
不结球白菜 热激蛋白 耐冷性 实时定量 抗氧化酶
Keywords:
Brassica campestris ssp.chinensis heat shock protein(HSP) chilling tolerance real-time quantitative PCR antioxidase
分类号:
S634.4
DOI:
10.7685/j.issn.1000-2030.2010.02.006
文献标志码:
A
摘要:
以不结球白菜暑绿为试材,研究了热激处理(42℃)诱导不结球白菜热激蛋白(BcHSP)合成与耐冷的相关性。结果表明:热激处理明显增强了不结球白菜幼苗的耐冷性,降低了膜伤害程度。超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性含量增加,而丙二醛(MDA)含量下降。已从热激诱导的不结球白菜中克隆了小分子量BcHSP基因(日本DNA数据库DDBJ登录号:AB367955)。荧光实时定量PCR分析表明BcHSP的表达量与耐冷性相一致。低温(4℃)胁迫下,在大肠杆菌中异源表达BcHSP可维持大肠杆菌较高的细胞活力,这些结果表明BcHSP合成与不结球白菜耐冷性的提高有关。
Abstract:
The relationship between heat shock protein expression and plant chilling tolerance was studied in Brassica campestris ssp. chinensis’Shulü’. Heat shock(42 ℃)applied to Brassica campestris ssp. chinensis seedling increased the chilling tolerance of seedling and decreased the permeability of cellular membranes. The activities of superoxide dismutase, catalase and peroxidase increased, and in contrast, the malondialdehyde content depressed. A full length cDNA of small heat shock protein(sHSP)was isolated from Brassica campestris ssp. chinensis with 42 ℃ heat shock. The gene was designated as BcHSP(DDBJ accession number AB367955). Real-time quantitative PCR analysis revealed that the BcHSP gene expression was coincided with chilling tolerance of seedling. The heterologous expression of BcHSP could maintain higher cell viability of Escherichia coli under low temperature stress(4 ℃). These results indicated that the BcHSP synthesis was correlated with the increased chilling tolerance of Brassica campestris ssp. chinensis seedling.

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更新日期/Last Update: 2010-03-31