[1]班青宇,耿建峰,侯喜林,等.不结球白菜叶片脯氨酸与可溶性蛋白含量的QTL分析[J].南京农业大学学报,2010,33(2):35-38.[doi:10.7685/j.issn.1000-2030.2010.02.007]
 BAN Qing-yu,GENG Jian-feng,HOU Xi-lin,et al.QTL mapping for proline and soluble protein content of leaves in non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2010,33(2):35-38.[doi:10.7685/j.issn.1000-2030.2010.02.007]
点击复制

不结球白菜叶片脯氨酸与可溶性蛋白含量的QTL分析()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
33卷
期数:
2010年2期
页码:
35-38
栏目:
出版日期:
2010-03-31

文章信息/Info

Title:
QTL mapping for proline and soluble protein content of leaves in non-heading Chinese cabbage
作者:
班青宇耿建峰侯喜林成妍王倩
南京农业大学农业部南方蔬菜遗传改良重点开放实验室,江苏,南京,210095;河南省农业科学院园艺研究所,河南,郑州,450002
Author(s):
BAN Qing-yu GENG Jian-feng HOU Xi-lin CHENG Yan WANG Qian
Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; Horticulture Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
关键词:
不结球白菜 脯氨酸 可溶性蛋白 QTL定位
Keywords:
non-heading Chinese cabbage proline soluble protein QTL mapping
分类号:
S634.3
DOI:
10.7685/j.issn.1000-2030.2010.02.007
文献标志码:
A
摘要:
用不结球白菜杂交品种暑绿的112个双单倍体纯系构建了包含186个分子标记的遗传连锁图谱,利用复合区间作图法检测了叶片脯氨酸含量和可溶性蛋白含量两性状的数量性状基因座(QTL)。结果表明:叶片脯氨酸含量由位于第5连锁群上与分子标记C20相距7.2cM的QTL控制,其加性效应和贡献率分别为0.37%和8.32%;可溶性蛋白含量由位于第3连锁群上与分子标记AE153相距0.8cM的QTL控制,其加性效应和贡献率分别为0.004%和8.12%。
Abstract:
In this study, an attempt was made to detect quantitative trait locus(QTL)for the leaf proline content and soluble protein content in a double haploid(DH)population derived from F1 hybrid variety Shulü by using composite interval mapping approach, based on a genetic linkage map with 186 molecular markers. The result showed that one QTL was detected for each of the two traits above. For leaf proline content, the QTL, with additive effect of 0.37% and the proportion of the total phenotypic variation explained of 8.32%, was placed on 7.2 cM apart from maker C20 on the fifth linkage group;and for soluble protein content, the QTL, with additive effect of 0.004% and the percent of the total phenotypic variance explained of 8.12%, was located on 0.8 cM close to marker AE15-3 on the third linkage group.

参考文献/References:

[1]侯喜林,张蜀宁. 优质不结球白菜新品种暑绿的选育[J]. 南京农业大学学报, 2005
[2]侯喜林. 不结球白菜育种研究新进展[J]. 南京农业大学学报, 2003
[3]全先庆,单雷. 脯氨酸在植物生长和非生物胁迫耐受中的作用[J]. 生物技术通讯, 2007
[4]全先庆,单雷. 高等植物脯氨酸代谢研究进展[J]. 生物技术通报, 2007
[5]Gleeson,D,Parkinson,M,Parkinson,M. Overproduction of proline in transgenic hybrid larch (Larix leptoeuropaea (Dengler[J]. Molecular Breeding, 2005
[6]律秀娜,黄真真. 干旱胁迫对月见草体内游离脯氨酸含量的影响[J]. 高师理科学刊, 2007
[7]夏晗. 低温、干旱和盐胁迫下沙冬青幼苗脯氨酸含量的变化[J]. 吉林林业科技, 2007
[8]Inouye,S. Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system[J]. Biochemical and Biophysical Research Communications, 2008
[9]毛桂莲,孙婕. NaCl胁迫下枸杞愈伤组织可溶性蛋白含量的变化[J]. 宁夏大学学报, 2005
[10]康俊梅,樊奋成. 干旱对苜蓿叶片可溶性蛋白的影响[J]. 草地学报, 2005
[11]耿建峰,张晓伟. 利用DH群体构建不结球白菜遗传连锁图谱[J]. 南京农业大学学报, 2007
[12]李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000
[13]Zeng,Z,B. Precision mapping of quantitative trait loci[J]. Genetics, 1994
[14]Churchill,G,A. Empirical threshold values for quantitative trait mapping[J]. Genetics, 1994
[15]Doerge,R,W. Permutation tests for multiple loci affecting a quantitative character[J]. Genetics, 1996
[16]蔡健. 大白菜叶游离脯氨酸含量变化的研究[J]. 安徽农业科学, 2006
[17]Miller,G,Honig,A,Honig,A. Responsive modes of Medicago sativa proline dehydrogenase genes during salt stress and recovery dictate free proline accumulation[J]. Planta, 2005
[18]陈璇,马纪. 低温胁迫对春小麦和冬小麦叶片游离脯氨酸含量变化的影响[J]. 新疆农业科学, 2007
[19]Zhu,J. Analysis of conditional genetic effects and variance components in developmental genetics[J]. Genetics, 1995

相似文献/References:

[1]张爱芬,王立,侯喜林,等.不结球白菜S 位点受体激酶基因片段的克隆与表达分析[J].南京农业大学学报,2011,34(3):25.[doi:10.7685/j.issn.1000-2030.2011.03.005]
 ZHANG Ai-fen,WANG Li,HOU Xi-lin,et al.Cloning and expression analysis of SRK gene fragment in non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2011,34(2):25.[doi:10.7685/j.issn.1000-2030.2011.03.005]
[2]黄建凤,徐小梦,沈其荣,等.2个不结球白菜品种硝酸盐累积差异的生理机制[J].南京农业大学学报,2011,34(1):74.[doi:10.7685/j.issn.1000-2030.2011.01.014]
 HUANG Jian-feng,XU Xiao-meng,SHEN Qi-rong,et al.Physiological mechanisms for the difference of nitrate accumulation in two cultivars of non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2011,34(2):74.[doi:10.7685/j.issn.1000-2030.2011.01.014]
[3]陈以博,侯喜林,陈晓峰.不结球白菜幼苗耐热性机制初步研究[J].南京农业大学学报,2010,33(1):27.[doi:10.7685/j.issn.1000-2030.2010.01.006]
 CHEN Yi-bo,HOU Xi-lin,CHEN Xiao-feng.Studies on heat tolerance mechanism of non-heading Chinese cabbage(Brassica campestris ssp.chinensis)[J].Journal of Nanjing Agricultural University,2010,33(2):27.[doi:10.7685/j.issn.1000-2030.2010.01.006]
[4]郑佳秋,侯喜林,朱红芳.热激诱导不结球白菜热激蛋白合成与耐冷性分析[J].南京农业大学学报,2010,33(2):30.[doi:10.7685/j.issn.1000-2030.2010.02.006]
 ZHENG Jia-qiu,HOU Xi-lin,ZHU hong-fang.Induction of heat shock protein synthesis and chilling tolerance in Brassica campestris ssp.chinensis[J].Journal of Nanjing Agricultural University,2010,33(2):30.[doi:10.7685/j.issn.1000-2030.2010.02.006]
[5]郝慧楠,王倩,侯喜林,等.不结球白菜主要农艺性状的分离分析[J].南京农业大学学报,2010,33(4):8.[doi:10.7685/j.issn.1000-2030.2010.04.002]
 HAO Hui-nan,WANG Qian,HOU Xi-lin,et al.Segregation analysis of the main agronomic characters of non-heading Chinese cabbage[J].Journal of Nanjing Agricultural University,2010,33(2):8.[doi:10.7685/j.issn.1000-2030.2010.04.002]
[6]荣子龙,侯喜林,史公军,等.不结球白菜晚抽薹BcFLC1基因克隆及表达分析[J].南京农业大学学报,2010,33(6):23.[doi:10.7685/j.issn.1000-2030.2010.06.005]
 RONG Zi-long,HOU Xi-lin,SHI Gong-jun,et al.Cloning and expression analysis of late bolting BcFLC1 gene from Brassica campestris ssp.chinensis[J].Journal of Nanjing Agricultural University,2010,33(2):23.[doi:10.7685/j.issn.1000-2030.2010.06.005]
[7]申娜娜,侯喜林.不结球白菜感染霜霉病菌后防御物质及酶的变化[J].南京农业大学学报,2009,32(1):23.[doi:10.7685/j.issn.1000-2030.2009.01.005]
 SHEN Shan-na,HOU Xi-lin.Changes of protective substances and enzymes in non-heading Chinese cabbage after infection by downy mildew[J].Journal of Nanjing Agricultural University,2009,32(2):23.[doi:10.7685/j.issn.1000-2030.2009.01.005]
[8]成妍,班青宇,王倩,等.不结球白菜游离小孢子培养及再生植株的倍性鉴定[J].南京农业大学学报,2009,32(2):25.[doi:10.7685/j.issn.1000-2030.2009.02.006]
 CHENG Yan,BAN Qing-yu,WANG Qian,et al.Isolated microspore culture and ploidy identification of microspore-derived plants in Brassica campestris ssp.chinensis[J].Journal of Nanjing Agricultural University,2009,32(2):25.[doi:10.7685/j.issn.1000-2030.2009.02.006]
[9]刘琳,侯喜林,王利英,等.不结球白菜感染芜菁花叶病毒后4种防御酶活性变化及其抗病相关性[J].南京农业大学学报,2009,32(3):14.[doi:10.7685/j.issn.1000-2030.2009.03.003]
 LIU Lin,HOU Xi-lin,WANG Li-ying,et al.Changes of four protective enzyme activities and relationships to resistance in non-heading Chinese cabbage after infection of Turnip mosaic virus[J].Journal of Nanjing Agricultural University,2009,32(2):14.[doi:10.7685/j.issn.1000-2030.2009.03.003]
[10]钟军华,张颖,卢之颖,等.盐胁迫下不结球白菜幼苗叶片内肽酶同工酶变化及生化特性研究[J].南京农业大学学报,2009,32(3):42.[doi:10.7685/j.issn.1000-2030.2009.03.008]
 ZHONG Jun-hua,ZHANG Ying,LU Zhi-ying,et al.The changes and biochemical characteristics of endopeptidase isozymes in non-heading Chinese cabbage under salt stress[J].Journal of Nanjing Agricultural University,2009,32(2):42.[doi:10.7685/j.issn.1000-2030.2009.03.008]

备注/Memo

备注/Memo:
国家“十一五”科技支撑计划项目(2006BAD01A7-1-11)
更新日期/Last Update: 2010-03-31