[1]陈志德,仲维功,王军,等.水稻苗期Cd2+胁迫的QTL定位研究[J].南京农业大学学报,2010,33(3):1-5.[doi:10.7685/j.issn.1000-2030.2010.03.001]
　CHEN Zhi-de,ZHONG Wei-gong,WANG Jun,et al.Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2010,33(3):1-5.[doi:10.7685/j.issn.1000-2030.2010.03.001]
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水稻苗期Cd2+胁迫的QTL定位研究()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
33卷
期数:
2010年3期
页码:
1-5
栏目:
出版日期:
2010-05-31

文章信息/Info

Title:
Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)
作者:
陈志德; 仲维功; 王军; 王洲飞; 张红生
南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095;江苏省农业科学院粮食作物研究所,江苏,南京,210014
Author(s):
CHEN Zhi-de;  ZHONG Wei-gong;  WANG Jun;  WANG Zhou-fei;  ZHANG Hong-sheng
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
水稻;  苗期;  Cd2+胁迫;  QTL定位
Keywords:
rice;  seedling stage;  Cd2+ stress;  QTLs mapping
分类号:
X503.231
DOI:
10.7685/j.issn.1000-2030.2010.03.001
文献标志码:
A
摘要:
利用韭菜青×IR26杂交后代建立一个重组自交系群体(recombinant inbred line,RIL)及相应的SSR分子标记连锁图谱,在2007和2008年进行Cd2+(5 mg.kg-1)胁迫下水稻幼苗耐Cd2+胁迫的QTL分析。结果表明:2007年检测到3个与幼苗耐Cd2+胁迫有关的QTLs,包括1个控制相对苗高的位点qRSH-7和2个控制单株相对干重的位点qRDW-11a和qRDW-11b,分别位于第7和第11染色体;2008年检测到6个与幼苗耐Cd2+胁迫相关的QTLs,包括3个控制单株相对干重的位点qRDW-1、qRDW-2和qRDW-7,1个控制相对苗高的位点qRSH-2,2个控制相对鲜重的位点qRFW-2和qRFW-7,分别位于第1、2、7染色体。在定位的9个QTLs中,qRDW-7和qRFW-7都位于第7染色体的RM6872与RM11标记之间,加性效应分别为4.89和5.44,表型贡献率为18.02%和15.24%;qRSH-7和qRDW-1的加性效应分别为5.09和-3.64,表型贡献率为13.48%和10.06%;其余位点表型贡献率均低于10.0%。
Abstract:
The recombinant inbred line(RIL) populations derived from Jiucaiqing×IR26 and their genetic linkage maps were employed to map QTL controlling tolerance to Cd2+ stress at rice seedlings with 5 mg·kg-1 by using the relative root length, relative seedling height, relative dry weight per plant and relative fresh weight per plant. The results showed that 3 QTLs on chromosome 7 and 11 were detected based on relative seedling height and relative dry weight per plant in 2007, i. e. qRSH-7, qRDW-11a and qRDW-11b. 6 QTLs on chromosome 1, 2 and 7 were detected based on relative root length, relative seedling height, relative dry weight per plant and relative fresh weight per plant in 2008, i. e. qRDW-1, qRSH-2, qRFW-2, qRFW-7, qRDW-2 and qRDW-7.Among the 9 QTLs, qRDW-7 and qRFW-7, on chromosome 7, explained 18.02% and 15.24% of the total phenotypic variation, and the additive effects of those QTLs were 4.89 and 5.44 respectively. qRSH-7 and qRDW-1 explained 13.48% and 10.06% of the total phenotypic variation, and the additive effects of those QTLs were 5.09 and-3.64 respectively. The other QTLs explained below 10.0% of the total phenotypic variation respectively.

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备注/Memo

备注/Memo:
江苏省农业三项工程项目(sx(2006)123)
更新日期/Last Update: 2010-05-30