[1]晋玉宽,杨世湖,余丽,等.不同启动子驱动下Pib基因的表达及与稻瘟病抗性的关系[J].南京农业大学学报,2010,33(4):1-7.[doi:10.7685/j.issn.1000-2030.2010.04.001]
 JIN Yu-kuan,YANG Shi-hu,YU Li,et al.Expression and resistance analysis of the Pib gene in transgenic rice under different promoters[J].Journal of Nanjing Agricultural University,2010,33(4):1-7.[doi:10.7685/j.issn.1000-2030.2010.04.001]
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不同启动子驱动下Pib基因的表达及与稻瘟病抗性的关系()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
33卷
期数:
2010年4期
页码:
1-7
栏目:
出版日期:
2010-07-30

文章信息/Info

Title:
Expression and resistance analysis of the Pib gene in transgenic rice under different promoters
作者:
晋玉宽杨世湖余丽赵宝泉万建民
南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,江苏,南京,210095
Author(s):
JIN Yu-kuan YANG Shi-hu YU Li ZHAO Bao-quan WAN Jian-min
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
关键词:
水稻 转基因 Pib基因 稻瘟病 启动子 ORF
Keywords:
rice transgene Pib gene rice blast promoter ORF
分类号:
S435.111.41
DOI:
10.7685/j.issn.1000-2030.2010.04.001
文献标志码:
A
摘要:
根据Pib基因的结构特点,分别构建了不同启动子驱动Pib基因ORF的表达载体pNAR701、pNAR704和pNAR705,分别由35S+Pib启动子、35S启动子和Pib启动子驱动;采用农杆菌介导转化水稻品种日本晴。经PCR、Southern blot分析证实了Pib基因已经稳定整合到日本晴的基因组中;Northern blot、实时荧光定量PCR对Pib基因表达分析表明:pNAR701转基因后代株系中目的基因的表达量较pNAR704和pNAR705高,且同一载体的不同转基因后代株系间存在着表达量的差异。对T1、T2代苗期稻瘟病抗性鉴定显示:不同启动子驱动的Pib结构基因mRNA的表达,都表现出对稻瘟菌生理小种ZB1和ZG1的高抗特性,但不同启动子驱动的该基因编码区mRNA的表达与稻瘟病抗性水平间没有明显差异。
Abstract:
According to the structure of Pib gene, we built different expression vectors named pNAR701, pNAR704 and pNAR705, respectively, and each drove the ORF of Pib gene by promoter of 35S + Pib, 35S and Pib. All constructions were done with Agrobacterium-mediated transformation into Nipponbare, and the results of PCR and Southern blot analysis confirmed that the Pib gene fragment has been inserted into the genome of Nipponbare. It showed that the expression of expected gene in pNAR701 transgenic lines was higher than that in pNAR704 and pNAR705, and the same transgenic event in different transgenic lines was various in expression of target gene by Northern blot, Real-time PCR and rice blast resistance analysis. In addition, the identification of blast resistance of T1 and T2 in seedling showed that 35S + Pib promoter, 35S promoter and endogenous promoter of Pib gene which drove the code region of Pib gene displayed high resistance to race ZB1 and ZG1, and there was no obvious difference between each other in resistance.

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备注/Memo

备注/Memo:
国家自然科学基金项目(30571044)
更新日期/Last Update: 2010-07-30