[1]荣子龙,侯喜林,史公军,等.不结球白菜晚抽薹BcFLC1基因克隆及表达分析[J].南京农业大学学报,2010,33(6):23-27.[doi:10.7685/j.issn.1000-2030.2010.06.005]
 RONG Zi-long,HOU Xi-lin,SHI Gong-jun,et al.Cloning and expression analysis of late bolting BcFLC1 gene from Brassica campestris ssp.chinensis[J].Journal of Nanjing Agricultural University,2010,33(6):23-27.[doi:10.7685/j.issn.1000-2030.2010.06.005]
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不结球白菜晚抽薹BcFLC1基因克隆及表达分析()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
33卷
期数:
2010年6期
页码:
23-27
栏目:
出版日期:
2010-11-30

文章信息/Info

Title:
Cloning and expression analysis of late bolting BcFLC1 gene from Brassica campestris ssp.chinensis
作者:
荣子龙侯喜林史公军肖栋郝慧楠
南京农业大学作物遗传与种质创新国家重点实验室/农业部南方蔬菜遗传改良重点开放实验室,江苏,南京,210095
Author(s):
RONG Zi-long HOU Xi-lin SHI Gong-jun XIAO Dong HAO Hui-nan
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
关键词:
不结球白菜 FLC基因 实时定量PCR 表达分析
Keywords:
Brassica campestris ssp. chinensis FLC gene real-time PCR expression analysis
分类号:
S634.3
DOI:
10.7685/j.issn.1000-2030.2010.06.005
文献标志码:
A
摘要:
采用RT-PCR、5’RACE和3’RACE方法,克隆得到了不结球白菜NJ074晚抽薹基因(BcFLC1)的cDNA全长序列。对BcFLC1基因所编码氨基酸序列的理化性质进行分析推测得到:该基因cDNA全长909bp,包含576bp的开放阅读框,编码191个氨基酸。不结球白菜BcFLC1蛋白功能域预测分析结果表明:该基因为MADS盒基因,其编码蛋白的1~60氨基酸属于MADS盒基因蛋白。荧光定量PCR分析表明:BcFLC1基因在不同生长发育阶段叶片中的表达情况不同,抽薹前高于抽薹后叶片中的表达量。BcFLC1基因在不同部位表达也存在差异,表达量从高到低依次为:叶、茎、花蕾、花和根。
Abstract:
A 909 bp full-length cDNA sequence of Brassica campestris flowering locus C1 ( BcFLC1) was obtained using RT-PCR, 3’ RACE and 5’RACE strategies. The full-length cDNA sequence of 909 bp in length contained an open reading frame of 576 bp encoding 191 amino acids, which had a typical MADS-box gene structure. The encoding protein from 1-60 amino acids belonged to MADS-box proteins. Real-time PCR analysis showed the expression level of the BcFLC1 gene in unbolting plant’s leaves was higher than that of bolting plant. The expression level of different tissues in bolting plant showed differences. The expression levels from high to low are leaf, stem, bud, flower and root.

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备注/Memo

备注/Memo:
江苏省自然科学基金项目(BK2009311);国家863计划项目(2006AA100108);国家科技支撑计划项目(2009BADB8B03-1)
更新日期/Last Update: 2010-11-30