XU Xiao-sa,LIU Xi,ZHAO Zhi-gang,et al.Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight[J].Journal of Nanjing Agricultural University,2011,34(1):8-13.[doi:10.7685/j.issn.1000-2030.2011.01.002]





Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight
XU Xiao-sa LIU Xi ZHAO Zhi-gang ZHOU Yu-jun WU Sheng-yang ZHOU Rong ZHANG Jun-jie JIANG Ling WAN Jian-min
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Research Center of Jiangsu Plant GeneEngineering, Nanjing Agricultural University, Nanjing 210095, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
水稻 重组自交系 SSR 分子标记 遗传连锁图谱 千粒重 染色体片段置换系 QTL
rice RILs SSR molecular marker genetic linkage map 1 000-grain weight CSSLs QTL
利用光、温敏核不育系培矮64S与常规品种93-11进行杂交,以单粒传方法建立含217个单株的重组自交系(R ILs)群体。选用775对SSR引物进行亲本多态性筛选,共有170对检测到多态性,频率为21.9%。构建的水稻分子遗传图谱共包含141个标记座位,总图距约2 060.4 cM,标记间平均图距为14.6 cM。群体中标记偏分离情况较严重。以该R ILs群体217个株系为材料,对千粒重性状进行了QTL分析。结果表明:在R ILs群体中检测到3个与千粒重相关的QTL,分别在第1、5、8染色体上,命名为qTGW-1、qTGW-5、qTGW-8,其LOD值为5.51、3.31、4.94,贡献率为17.90%、6.12%、9.59%。qTGW-1控制千粒重的增效基因来自低值亲本培矮64S,qTGW-5和qTGW-8控制千粒重的增效基因来自高值亲本93-11。通过相应的含有154个家系的全基因组染色体片段置换系(CSSLs)群体图示基因型分析,证实了在第1染色体上标记RM315附近存在控制千粒重的增效基因,来源于染色体片段供体亲本培矮64S,使千粒重增加1.03 g;在第5、8染色体上标记RM3663、RM310附近存在控制千粒重的增效基因,来自染色体片段受体亲本93-11,使千粒重分别增加0.63 g和0.80 g。
The present study used the photo-thermo sensitive genic male sterile Peiai 64S and 93-11 as the material to develop 217 recombinant inbred lines(RILs)by using SSR marker aided selection. A total of 775 pairs of SSR markers were used to analyze the polymorphism between Peiai 64S and 93-11.It was shown that there were 170 pairs of polymorphism markers and their polymorphic ratio are 21.9%. The linkage map consists of 141 SSR markers. This map covered the rice genome about 2 060.4 cM with about 14.6 cM of average interval. Serious segregation distortion was observed in this RILs population. QTL analysis for the 1 000-grain weight(TGW)was conducted with the RILs of Peiai 64S/93-11 with 217 lines and a genome-wide chromosome segment substitution lines(CSSLs)population with 154 lines. The result indicated that in the RILs population, three QTL controlling the TGW were detected on chromosome 1, 5 and 8, named qTGW-1, qTGW-5, qTGW-8, respectively. The LOD values of each QTL were 5.51, 3.31 and 4.94, and phenotypic variations were explained by each QTL are 17.90%, 6.12% and 9.59%, respectively. The allele of qTGW-1 was derived from Peiai 64S and the other two alleles of qTGW-5 and qTGW-8 were derived from 93-11.Analysis of CSSLs graphical gene types also showed that there was a positive allele on the Peiai 64S chromosome substitution segment near the marker RM315 locating on the chromosome 1 which certificated the existence of qTGW-1, and also that there were other two negative alleles on the Peiai 64S chromosome substitution segment near the marker RM3663 and RM310 locating on the chromosome 5 and 8 which proved the existence of the qTGW-5 and qTGW-8.


[1]鄂志国,庄杰云. 水稻千粒重QTL图谱整合研究[J]. 科技通报, 2008
[2]曹立勇,庄杰云. 水稻产量性状的QTL定位与上位性分析[J]. 中国农业科学, 2003
[3]Hittalmani,S,Bagali,P,G,Bagali,P,G. Molecular mapping of quantitative trait loci for plant growth,yield and yield related traits across three diverse locations in a doublcd haploid rice population[J]. Euphytica, 2002
[4]Yoon,D,B,Kim,H,J,Kim,H,J. Mapping quantitative trait loci for yield components and morphological traits in an advanced backeross population between Oryza grandiglumis and the O. sativa japonica cultivar Hwascongbyeo[J]. Theoretical and Applied Genetics, 2006
[5]Song,X,J,Shi,M,Shi,M. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase[J]. Nature Genetics, 2007
[6]邹江石,王才林. 两系杂交稻"两优培九"的选育及其栽培特性[J]. 中国农业科学, 2003
[7]Wang,W,W,Ge,X,M,Ge,X,M. Protcomic profiling of rice embryos from a hybrid rice cultivar and its parental lines[J]. Proteomics, 2008
[8]Yu,J,Wang,J,Wang,J. A dr’. fft sequence of the rice genome(Oryza sativa L. ssp. indica][J]. Science, 2002
[9]Xiao,Y,H,Jiang,L,Jiang,L. Development of the chromosome segment substitution lines(CSSLs]derived from a hybrid rice cross,Peiai64S/93-11 with super high yield potential[J]. Rice Genetics Newsletter, 2005
[10]Dellaporta,S,L,Hicks,J,B,Hicks,J,B. A plant DNA minipreparation:version I1[J]. Plant Molecular Biology Reporter, 1983
[11]Chen,X,Xu,Y,Xu,Y. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L. ][J]. Theoretical and Applied Genetics, 1997
[12]Wang,S,C,Zeng,Z,B,Zeng,Z,B. Windows QTL Cartographer 2. 5 User Manual[EB/OL]blip://statgen. ncsu. edu/-shchwang/WinQTLCart,pdf, 2009-11-19
[13]Churchill,G,A. Empirical threshohl values for quantitative trait mapping[J]. Genetika, 1994
[14]McCouch,S,R,Yano,M,Yano,M. Report on QTL nomenclature[J]. Rice Genetics Newsletter, 1997
[15]Jiang,G,H,Li,X,H,Li,X,H. Characterization of the genetic basis for yield and its component traits of rice revealed by doubled haphfid population[J]. 遗传学报, 2004
[16]Thomson,M,J,McClung,A,M,McClung,A,M. Mapping quantitative trait loci for yield,yield components and morpholological traits in an advancedhackcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson[J]. Theoretical and Applied Genetics, 2003
[17]Moneada,P,Borrero,J,Borrero,J. Quantitative trait loci for yieht aml yield components in an Oryza sativaxOryza rufipogon BC2F2population evaluated in an upland envirnnment[J]. Theoretical and Applied Genetics, 2001
[18]Septiningsih,E,M,Lubis,E,Lubis,E. Identification of quantitative trait loci for yield and yield components in an advanced backerosspopulation derived from the Oryza sativa variety IR64 and the wiht relative O. rufipogon[J]. Theoretical and Applied Genetics, 2003
[19]Lu,C,F,Tan,Z,B,Tan,Z,B. Comparative mapping of QTLs for agronomic traits of rice across environments by using a doubled-haploid pop-ulation[J]. Theoretical and Applied Genetics, 1997
[20]Lin,H,X,Zhuang,J,Y,Zhuang,J,Y. RFLP mapping of QTLs for yield and related characters in rice(Oryza sativa L. ][J]. Theoretical and Applied Genetics, 1996
[21]Temnykh,S,Ayres,N,Ayres,N. Mapping and genome organization of microsatellite sequences in rice(Oryza sativa L. )[J]. Theoretical and Applied Genetics, 2000
[22]Tan,L,B,Liu,F,X,Liu,F,X. Quantitative trail loci underlying domestication and yield-related traits in an Qryza sativaxQryza rufipogon advanced backcross population[J]. Genome/National Research Council Canada=genome/Conseil National De Recherches Canada, 2008


 ZHANG Chen-ming,XU Ye-hong,ZHAO Hai-juan,et al.Effects of different nitrogen forms on nitrogen uptake and root growth of rice at the seedling stage[J].Journal of Nanjing Agricultural University,2011,34(1):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
 HAO Wen-ya,SHEN Qi-rong,RAN Wei,et al.The effects of sugars and amino acids in watermelon and rice root exudates on the growth of Fusarium oxysporum f.sp. niveum[J].Journal of Nanjing Agricultural University,2011,34(1):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
 WEI Guang-bin,XU Hai-gang,DING Yan-feng,et al.Development and realization of the rice design cultivation system[J].Journal of Nanjing Agricultural University,2011,34(1):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
 LI Gang-hua,WANG Hui-zhi,WANG Shao-hua,et al.Effect of nitrogen applied at rice panicle initiation stage on carbon and nitrogen metabolism and spikelets per panicle[J].Journal of Nanjing Agricultural University,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
 WANG Bi-qian,FAN Xiao-rong,XU Guo-hua,et al.Characteristics of flag leaf senescence among three rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(1):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
 ZHAO Cheng-guo,XU Hai-gang,LI Gang-hua,et al.Studies on population composition of super-high-yielding single-cropping japonica rice in heading stage[J].Journal of Nanjing Agricultural University,2011,34(1):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
 CHEN Zhi-de,ZHONG Wei-gong,WANG Jun,et al.Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
 YE Li-ting,FAN Jian-bo,XU Ye-hong,et al.Characteristics of growth in rice genotypes with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(1):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
 JIN Yu-kuan,YANG Shi-hu,YU Li,et al.Expression and resistance analysis of the Pib gene in transgenic rice under different promoters[J].Journal of Nanjing Agricultural University,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
 WANG Dong-sheng,FAN Jian-bo,YE Li-ting,et al.Canopy growth characteristics of rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(1):101.[doi:10.7685/j.issn.1000-2030.2010.05.019]
 ZHANG Lu-xia,WANG Song-feng,JIANG Ling,et al.QTL analysis of cold tolerance at the bud bursting period in rice(Oryza sativa L.) by using recombinant inbred lines[J].Journal of Nanjing Agricultural University,2007,30(1):1.[doi:10.7685/j.issn.1000-2030.2007.04.001]
 Xu Xiaofeng,Cheng Xianian,Luo Lijun,et al.Studies on resistance of recombinant inbred lines of Lemont/Teqing to brown planthopper[J].Journal of Nanjing Agricultural University,1999,22(1):25.[doi:10.7685/j.issn.1000-2030.1999.03.007]


更新日期/Last Update: 2011-01-30