[1]徐小飒,刘喜,赵志刚,等.培矮64S/93-11重组自交系分子图谱构建及千粒重QTL检测[J].南京农业大学学报,2011,34(1):8-13.[doi:10.7685/j.issn.1000-2030.2011.01.002]
 XU Xiao-sa,LIU Xi,ZHAO Zhi-gang,et al.Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight[J].Journal of Nanjing Agricultural University,2011,34(1):8-13.[doi:10.7685/j.issn.1000-2030.2011.01.002]
点击复制

培矮64S/93-11重组自交系分子图谱构建及千粒重QTL检测()
分享到:

《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年1期
页码:
8-13
栏目:
出版日期:
2011-02-28

文章信息/Info

Title:
Construction of genetic linkage map based on a RILs population derived from the hybrid rice Peiai 64S/93-11 and detection of QTL for 1000-grain weight
作者:
徐小飒刘喜赵志刚周裕军吴盛阳周蓉张俊杰江玲万建民
南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,江苏,南京,210095;中国农业科学院作物科学研究所,北京,100081
Author(s):
XU Xiao-sa LIU Xi ZHAO Zhi-gang ZHOU Yu-jun WU Sheng-yang ZHOU Rong ZHANG Jun-jie JIANG Ling WAN Jian-min
State Key Laboratory of Crop Genetics and Germplasm Enhancement/Research Center of Jiangsu Plant GeneEngineering, Nanjing Agricultural University, Nanjing 210095, China; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
关键词:
水稻 重组自交系 SSR 分子标记 遗传连锁图谱 千粒重 染色体片段置换系 QTL
Keywords:
rice RILs SSR molecular marker genetic linkage map 1 000-grain weight CSSLs QTL
分类号:
S511
DOI:
10.7685/j.issn.1000-2030.2011.01.002
文献标志码:
A
摘要:
利用光、温敏核不育系培矮64S与常规品种93-11进行杂交,以单粒传方法建立含217个单株的重组自交系(R ILs)群体。选用775对SSR引物进行亲本多态性筛选,共有170对检测到多态性,频率为21.9%。构建的水稻分子遗传图谱共包含141个标记座位,总图距约2 060.4 cM,标记间平均图距为14.6 cM。群体中标记偏分离情况较严重。以该R ILs群体217个株系为材料,对千粒重性状进行了QTL分析。结果表明:在R ILs群体中检测到3个与千粒重相关的QTL,分别在第1、5、8染色体上,命名为qTGW-1、qTGW-5、qTGW-8,其LOD值为5.51、3.31、4.94,贡献率为17.90%、6.12%、9.59%。qTGW-1控制千粒重的增效基因来自低值亲本培矮64S,qTGW-5和qTGW-8控制千粒重的增效基因来自高值亲本93-11。通过相应的含有154个家系的全基因组染色体片段置换系(CSSLs)群体图示基因型分析,证实了在第1染色体上标记RM315附近存在控制千粒重的增效基因,来源于染色体片段供体亲本培矮64S,使千粒重增加1.03 g;在第5、8染色体上标记RM3663、RM310附近存在控制千粒重的增效基因,来自染色体片段受体亲本93-11,使千粒重分别增加0.63 g和0.80 g。
Abstract:
The present study used the photo-thermo sensitive genic male sterile Peiai 64S and 93-11 as the material to develop 217 recombinant inbred lines(RILs)by using SSR marker aided selection. A total of 775 pairs of SSR markers were used to analyze the polymorphism between Peiai 64S and 93-11.It was shown that there were 170 pairs of polymorphism markers and their polymorphic ratio are 21.9%. The linkage map consists of 141 SSR markers. This map covered the rice genome about 2 060.4 cM with about 14.6 cM of average interval. Serious segregation distortion was observed in this RILs population. QTL analysis for the 1 000-grain weight(TGW)was conducted with the RILs of Peiai 64S/93-11 with 217 lines and a genome-wide chromosome segment substitution lines(CSSLs)population with 154 lines. The result indicated that in the RILs population, three QTL controlling the TGW were detected on chromosome 1, 5 and 8, named qTGW-1, qTGW-5, qTGW-8, respectively. The LOD values of each QTL were 5.51, 3.31 and 4.94, and phenotypic variations were explained by each QTL are 17.90%, 6.12% and 9.59%, respectively. The allele of qTGW-1 was derived from Peiai 64S and the other two alleles of qTGW-5 and qTGW-8 were derived from 93-11.Analysis of CSSLs graphical gene types also showed that there was a positive allele on the Peiai 64S chromosome substitution segment near the marker RM315 locating on the chromosome 1 which certificated the existence of qTGW-1, and also that there were other two negative alleles on the Peiai 64S chromosome substitution segment near the marker RM3663 and RM310 locating on the chromosome 5 and 8 which proved the existence of the qTGW-5 and qTGW-8.

参考文献/References:

[1]鄂志国,庄杰云. 水稻千粒重QTL图谱整合研究[J]. 科技通报, 2008
[2]曹立勇,庄杰云. 水稻产量性状的QTL定位与上位性分析[J]. 中国农业科学, 2003
[3]Hittalmani,S,Bagali,P,G,Bagali,P,G. Molecular mapping of quantitative trait loci for plant growth,yield and yield related traits across three diverse locations in a doublcd haploid rice population[J]. Euphytica, 2002
[4]Yoon,D,B,Kim,H,J,Kim,H,J. Mapping quantitative trait loci for yield components and morphological traits in an advanced backeross population between Oryza grandiglumis and the O. sativa japonica cultivar Hwascongbyeo[J]. Theoretical and Applied Genetics, 2006
[5]Song,X,J,Shi,M,Shi,M. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase[J]. Nature Genetics, 2007
[6]邹江石,王才林. 两系杂交稻"两优培九"的选育及其栽培特性[J]. 中国农业科学, 2003
[7]Wang,W,W,Ge,X,M,Ge,X,M. Protcomic profiling of rice embryos from a hybrid rice cultivar and its parental lines[J]. Proteomics, 2008
[8]Yu,J,Wang,J,Wang,J. A dr’. fft sequence of the rice genome(Oryza sativa L. ssp. indica][J]. Science, 2002
[9]Xiao,Y,H,Jiang,L,Jiang,L. Development of the chromosome segment substitution lines(CSSLs]derived from a hybrid rice cross,Peiai64S/93-11 with super high yield potential[J]. Rice Genetics Newsletter, 2005
[10]Dellaporta,S,L,Hicks,J,B,Hicks,J,B. A plant DNA minipreparation:version I1[J]. Plant Molecular Biology Reporter, 1983
[11]Chen,X,Xu,Y,Xu,Y. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L. ][J]. Theoretical and Applied Genetics, 1997
[12]Wang,S,C,Zeng,Z,B,Zeng,Z,B. Windows QTL Cartographer 2. 5 User Manual[EB/OL]blip://statgen. ncsu. edu/-shchwang/WinQTLCart,pdf, 2009-11-19
[13]Churchill,G,A. Empirical threshohl values for quantitative trait mapping[J]. Genetika, 1994
[14]McCouch,S,R,Yano,M,Yano,M. Report on QTL nomenclature[J]. Rice Genetics Newsletter, 1997
[15]Jiang,G,H,Li,X,H,Li,X,H. Characterization of the genetic basis for yield and its component traits of rice revealed by doubled haphfid population[J]. 遗传学报, 2004
[16]Thomson,M,J,McClung,A,M,McClung,A,M. Mapping quantitative trait loci for yield,yield components and morpholological traits in an advancedhackcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson[J]. Theoretical and Applied Genetics, 2003
[17]Moneada,P,Borrero,J,Borrero,J. Quantitative trait loci for yieht aml yield components in an Oryza sativaxOryza rufipogon BC2F2population evaluated in an upland envirnnment[J]. Theoretical and Applied Genetics, 2001
[18]Septiningsih,E,M,Lubis,E,Lubis,E. Identification of quantitative trait loci for yield and yield components in an advanced backerosspopulation derived from the Oryza sativa variety IR64 and the wiht relative O. rufipogon[J]. Theoretical and Applied Genetics, 2003
[19]Lu,C,F,Tan,Z,B,Tan,Z,B. Comparative mapping of QTLs for agronomic traits of rice across environments by using a doubled-haploid pop-ulation[J]. Theoretical and Applied Genetics, 1997
[20]Lin,H,X,Zhuang,J,Y,Zhuang,J,Y. RFLP mapping of QTLs for yield and related characters in rice(Oryza sativa L. ][J]. Theoretical and Applied Genetics, 1996
[21]Temnykh,S,Ayres,N,Ayres,N. Mapping and genome organization of microsatellite sequences in rice(Oryza sativa L. )[J]. Theoretical and Applied Genetics, 2000
[22]Tan,L,B,Liu,F,X,Liu,F,X. Quantitative trail loci underlying domestication and yield-related traits in an Qryza sativaxQryza rufipogon advanced backcross population[J]. Genome/National Research Council Canada=genome/Conseil National De Recherches Canada, 2008

相似文献/References:

[1]张辰明,徐烨红,赵海娟,等.不同氮形态对水稻苗期氮素吸收和根系生长的影响[J].南京农业大学学报,2011,34(3):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
 ZHANG Chen-ming,XU Ye-hong,ZHAO Hai-juan,et al.Effects of different nitrogen forms on nitrogen uptake and root growth of rice at the seedling stage[J].Journal of Nanjing Agricultural University,2011,34(1):72.[doi:10.7685/j.issn.1000-2030.2011.03.013]
[2]郝文雅,沈其荣,冉炜,等.西瓜和水稻根系分泌物中糖和氨基酸对西瓜枯萎病病原菌生长的影响[J].南京农业大学学报,2011,34(3):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
 HAO Wen-ya,SHEN Qi-rong,RAN Wei,et al.The effects of sugars and amino acids in watermelon and rice root exudates on the growth of Fusarium oxysporum f.sp. niveum[J].Journal of Nanjing Agricultural University,2011,34(1):77.[doi:10.7685/j.issn.1000-2030.2011.03.014]
[3]魏广彬,徐海港,丁艳峰,等.水稻设计栽培系统的研制与实现[J].南京农业大学学报,2011,34(1):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
 WEI Guang-bin,XU Hai-gang,DING Yan-feng,et al.Development and realization of the rice design cultivation system[J].Journal of Nanjing Agricultural University,2011,34(1):14.[doi:10.7685/j.issn.1000-2030.2011.01.003]
[4]李刚华,王惠芝,王绍华,等.穗肥对水稻穗分化期碳氮代谢及颖花数的影响[J].南京农业大学学报,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
 LI Gang-hua,WANG Hui-zhi,WANG Shao-hua,et al.Effect of nitrogen applied at rice panicle initiation stage on carbon and nitrogen metabolism and spikelets per panicle[J].Journal of Nanjing Agricultural University,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.01.001]
[5]王碧茜,范晓荣,徐国华,等.不同氮效率水稻品种旗叶的衰老特征[J].南京农业大学学报,2010,33(2):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
 WANG Bi-qian,FAN Xiao-rong,XU Guo-hua,et al.Characteristics of flag leaf senescence among three rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(1):8.[doi:10.7685/j.issn.1000-2030.2010.02.002]
[6]赵成国,徐海港,李刚华,等.超高产单季粳稻抽穗期群体构成研究[J].南京农业大学学报,2011,34(2):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
 ZHAO Cheng-guo,XU Hai-gang,LI Gang-hua,et al.Studies on population composition of super-high-yielding single-cropping japonica rice in heading stage[J].Journal of Nanjing Agricultural University,2011,34(1):23.[doi:10.7685/j.issn.1000-2030.2011.02.005]
[7]陈志德,仲维功,王军,等.水稻苗期Cd2+胁迫的QTL定位研究[J].南京农业大学学报,2010,33(3):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
 CHEN Zhi-de,ZHONG Wei-gong,WANG Jun,et al.Mapping of QTL of tolerance to Cd^{2+} stress at seedling stage in rice(Oryza sativa L.)[J].Journal of Nanjing Agricultural University,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.03.001]
[8]叶利庭,樊剑波,徐晔红,等.不同氮效率水稻的生长特性[J].南京农业大学学报,2010,33(3):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
 YE Li-ting,FAN Jian-bo,XU Ye-hong,et al.Characteristics of growth in rice genotypes with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(1):77.[doi:10.7685/j.issn.1000-2030.2010.03.015]
[9]晋玉宽,杨世湖,余丽,等.不同启动子驱动下Pib基因的表达及与稻瘟病抗性的关系[J].南京农业大学学报,2010,33(4):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
 JIN Yu-kuan,YANG Shi-hu,YU Li,et al.Expression and resistance analysis of the Pib gene in transgenic rice under different promoters[J].Journal of Nanjing Agricultural University,2010,33(1):1.[doi:10.7685/j.issn.1000-2030.2010.04.001]
[10]王东升,樊剑波,叶利庭,等.不同氮效率水稻品种的群体发育特征[J].南京农业大学学报,2010,33(5):101.[doi:10.7685/j.issn.1000-2030.2010.05.019]
 WANG Dong-sheng,FAN Jian-bo,YE Li-ting,et al.Canopy growth characteristics of rice cultivars with different nitrogen use efficiency[J].Journal of Nanjing Agricultural University,2010,33(1):101.[doi:10.7685/j.issn.1000-2030.2010.05.019]
[11]张露霞,王松风,江玲,等.利用重组自交系群体检测水稻芽期间耐冷性QTL[J].南京农业大学学报,2007,30(4):1.[doi:10.7685/j.issn.1000-2030.2007.04.001]
 ZHANG Lu-xia,WANG Song-feng,JIANG Ling,et al.QTL analysis of cold tolerance at the bud bursting period in rice(Oryza sativa L.) by using recombinant inbred lines[J].Journal of Nanjing Agricultural University,2007,30(1):1.[doi:10.7685/j.issn.1000-2030.2007.04.001]
[12]许晓风,程遐年,罗利军,等.水稻Lemont/Teqing重组自交系对褐飞虱的抗性表现[J].南京农业大学学报,1999,22(3):25.[doi:10.7685/j.issn.1000-2030.1999.03.007]
 Xu Xiaofeng,Cheng Xianian,Luo Lijun,et al.Studies on resistance of recombinant inbred lines of Lemont/Teqing to brown planthopper[J].Journal of Nanjing Agricultural University,1999,22(1):25.[doi:10.7685/j.issn.1000-2030.1999.03.007]

备注/Memo

备注/Memo:
国家863计划项目(2009AA101101,2007AA10Z116);国家科技支撑计划项目(2006BAD01A01);江苏省高技术招标项目(BE2009301-3,BE2008354,BE2008352);南京农业大学SRT计划项目(0801A27)
更新日期/Last Update: 2011-01-30