[1]史建磊,娄群峰,钱春桃,等.黄瓜染色体片段导入系的构建与遗传评价[J].南京农业大学学报,2011,34(1):20-24.[doi:10.7685/j.issn.1000-2030.2011.01.004]
 SHI Jian-lei,LOU Qun-feng,QIAN Chun-tao,et al.Construction and genetic evaluation of chromosome segment introgression lines in cucumber[J].Journal of Nanjing Agricultural University,2011,34(1):20-24.[doi:10.7685/j.issn.1000-2030.2011.01.004]
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黄瓜染色体片段导入系的构建与遗传评价()
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《南京农业大学学报》[ISSN:1000-2030/CN:32-1148/S]

卷:
34卷
期数:
2011年1期
页码:
20-24
栏目:
出版日期:
2011-02-28

文章信息/Info

Title:
Construction and genetic evaluation of chromosome segment introgression lines in cucumber
作者:
史建磊娄群峰钱春桃万红建周晓慧陈劲枫
南京农业大学园艺学院/作物遗传与种质创新国家重点实验室,江苏,南京,210095
Author(s):
SHI Jian-lei LOU Qun-feng QIAN Chun-tao WAN Hong-jian ZHOU Xiao-hui CHEN Jin-feng
College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
关键词:
黄瓜 SSR标记 染色体片段导入系 评价
Keywords:
cucumber SSR markers chromosome segment introgression lines(CSILs) evaluation
分类号:
S642.2
DOI:
10.7685/j.issn.1000-2030.2011.01.004
文献标志码:
A
摘要:
以综合性状优良的栽培黄瓜(Cucumis sativus L.,2n=14)北京截头为受体亲本,以携带有多种抗性性状的黄瓜珍稀野生种酸黄瓜(Cucumis hystrix Chakr.,2n=24)为供体亲本,在本实验室前期获得种间杂交异源四倍体的基础上,通过多代回交和自交,结合SSR标记辅助选择构建了以北京截头为遗传背景的黄瓜野生种染色体片段导入系,并对其导入片段的数目、分布、大小和覆盖率等进行了评价。结果表明:该导入系群体携带的38个供体片段不均匀地分布于黄瓜7条染色体上,其长度介于1.1~14.9 cM,平均长度为5.3 cM,总长度为201.9 cM,在黄瓜基因组上的覆盖率为23.5%。
Abstract:
The cultivated cucumber(Cucumis sativus L. , 2n=14)Beijingjietou with comprehensive good traits and precious rare wild sour cucumber(Cucumis hystrix Chakr. , 2n=24)carrying a variety of resistance traits were used as receptor parent and donor parent respectively in the present study. Chromosome segment introgression lines(CSILs)were developed on the basis of interspecific allotetraploid by multiple generational backcrossing and selfing, combined with SSR marker-assisted selection(MAS), with the genetic background of Beijingjietou. By evaluating of the number, distribution, segment size and coverage rate, it showed that the 38 donor segments of CSILs existed unevenly in the seven chromosomes. Their lengths were between 1.1-14.9 cM, the average length was 5.3 cM, the total length was 201.9 cM and the coverage rate in the cucumber genome was 23.5%.

参考文献/References:

[1]王立秋,薛亚尔. 玉米衔接式单片段导入系群体的构建和评价[J]. 作物学报, 2007
[2]Ghesquièrc,A,Second,G,Second,G. First steps lowards a rational use of African rice,Oryza glaberrima,in rice breeding through a ’contig line’ concept[J]. Euphytica, 1997
[3]Bernacehi,D,Emmatty,D,Emmatty,D. Advanced baekcross QTL analysis of tomato. Ⅱ. Evaluation of near-isogenic lines carrying singledonor introgressions for desirable wild QTL-alleles derived from Lycopersicon hirsutum and L. pimpinellifolium[J]. Theoretical and Applied Genetics, 1998
[4]张丽霞,刘学义. 染色休单片段代换系的构建及应用于QTL精细定位[J]. 分子植物育种, 2004
[5]吴新儒,刘爱峰. 小麦重要农艺性状QTL近等基因导入系的选育[J]. 麦类作物学报, 2007
[6]Kennard,W,C,Dijkhuizen,A,Dijkhuizen,A. Linkages among RFLP,RAPD,isozyme,disease-resistance,anti morphological markers in narrow and wide crosses of cucumber[J]. Theoretical and Applied Genetics, 1994
[7]Fazio,G,Stevens,M,R,Stevens,M,R. Genetic mapping and QTL analysis of horticultural traits in cucumber(Cucumis sativus L. ]using recombinant inbred lines[J]. Theoretical and Applied Genetics, 2003
[8]王刚,李效尊. 黄瓜SRAP遗传连锁图的构建及侧枝基因定位[J]. 中国科学C辑, 2004
[9]潘俊松,李效尊. 黄瓜SRAP遗传连锁图的构建及始花节位的基凶定位[J]. 自然科学进展, 2005
[10]Chen,J,F. A new synthetic species of Cueumis(Cucurbitaceae]from interpecific hybridization and chromosome doubling[J]. Brittonia, 2000
[11]Chen,J,F,Tashiro,Y,Tashiro,Y. Successful interspecific hybridization between Cucumis sativus L. and C. hystrix Chakr[J]. Euphytica, 1997
[12]Murray,M,G. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research, 1980
[13]Katzir,N,Tzuri,G,Tzuri,G. Length polymorphisrn and homologies of microsatellites in several Cucurbitaceae species[J]. Theoretical and Applied Genetics, 1996
[14]Charters,Y,M,Wilkinson,M,J,Wilkinson,M,J. PCR analysis of oilseed rape cultivars(Brassica napus L. ssp. oleifera]using 5’ -anchored simple sequcnce repeat (SSR]primers[J]. Theoretical and Applied Genetics, 1996
[15]Ren,Y,Liu,J,H,Liu,J,H. An integrated genetic and cytogenetic map of the cucumber genome[J]. PLoS One, 2009
[16]Young,N,D. Restriction fragment length polymorphism maps and Ihe concept of graphical genotypes[J]. Theoretical and Applied Genetics, 1989
[17]何风华,曾瑞珍. 利用高代回交和分子标记辅助选择建立水稻单片段代换系[J]. 遗传学报, 2005
[18]Hospital,F,Mulsant,P,Mulsant,P. Using markers in gene introgression breeding programs[J]. Genetics, 1992
[19]夏军红. 玉米R13近等基因系的分子标记辅助回交选育与效益分析[J]. 作物学报, 2002
[20]李文涛,张泽民. 水稻F《,1》花粉不育性近等基因系导人片段的分析[J]. 中国水稻科学, 2003
[21]赵明辉,徐正进. 水稻单片段代换系群体的构建及研究进展[J]. 云南农业大学学报, 2007
[22]廖长见,潘光堂. 作物染色体导入系的构建及其应用[J]. 分子植物育种, 2007
[23]陈龙正,娄群峰. 野黄瓜与栽培黄瓜正反交异源四倍体序列消除的初步研究[J]. 植物分类学报, 2006

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备注/Memo

备注/Memo:
国家自然科学基金项目(30671419,30700541),国家自然科学基金重点项目(30830079);国家高技术研究发展计划专项经费(2008AA10Z150,2006AA10Z1A8,2006AA100108);作物遗传与种质创新国家重点实验室开放基金项目(ZW2007010)
更新日期/Last Update: 2011-01-30